Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling
Chemerin, a pleiotropic adipokine coded by the <i>RARRES2</i> gene, has been reported to affect the pathophysiology of various cancer entities. To further approach the role of this adipokine in ovarian cancer (OC), intratumoral protein levels of chemerin and its receptor chemokine-like r...
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MDPI AG
2023-03-01
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Online Access: | https://www.mdpi.com/2075-4418/13/5/944 |
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author | Florian Weber Susanne Schueler-Toprak Christa Buechler Olaf Ortmann Oliver Treeck |
author_facet | Florian Weber Susanne Schueler-Toprak Christa Buechler Olaf Ortmann Oliver Treeck |
author_sort | Florian Weber |
collection | DOAJ |
description | Chemerin, a pleiotropic adipokine coded by the <i>RARRES2</i> gene, has been reported to affect the pathophysiology of various cancer entities. To further approach the role of this adipokine in ovarian cancer (OC), intratumoral protein levels of chemerin and its receptor chemokine-like receptor 1 (CMKLR1) were examined by immunohistochemistry analyzing tissue microarrays with tumor samples from 208 OC patients. Since chemerin has been reported to affect the female reproductive system, associations with proteins involved in steroid hormone signaling were analyzed. Additionally, correlations with ovarian cancer markers, cancer-related proteins, and survival of OC patients were examined. A positive correlation of chemerin and CMKLR1 protein levels in OC (Spearman’s rho = 0.6, <i>p</i> < 0.0001) was observed. Chemerin staining intensity was strongly associated with the expression of progesterone receptor (PR) (Spearman´s rho = 0.79, <i>p</i> < 0.0001). Both chemerin and CMKLR1 proteins positively correlated with estrogen receptor β (ERβ) and estrogen-related receptors. Neither chemerin nor the CMKLR1 protein level was associated with the survival of OC patients. At the mRNA level, in silico analysis revealed low <i>RARRES2</i> and high <i>CMKLR1</i> expression associated with longer overall survival. The results of our correlation analyses suggested the previously reported interaction of chemerin and estrogen signaling to be present in OC tissue. Further studies are needed to elucidate to which extent this interaction might affect OC development and progression. |
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id | doaj.art-7877aed129774159820fbc4adad956e5 |
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issn | 2075-4418 |
language | English |
last_indexed | 2024-03-11T07:27:42Z |
publishDate | 2023-03-01 |
publisher | MDPI AG |
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series | Diagnostics |
spelling | doaj.art-7877aed129774159820fbc4adad956e52023-11-17T07:30:18ZengMDPI AGDiagnostics2075-44182023-03-0113594410.3390/diagnostics13050944Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen SignalingFlorian Weber0Susanne Schueler-Toprak1Christa Buechler2Olaf Ortmann3Oliver Treeck4Institute for Pathology, University of Regensburg, 93053 Regensburg, GermanyDepartment of Gynecology and Obstetrics, University Medical Center Regensburg, 93053 Regensburg, GermanyDepartment of Internal Medicine I, University Medical Center Regensburg, 93053 Regensburg, GermanyDepartment of Gynecology and Obstetrics, University Medical Center Regensburg, 93053 Regensburg, GermanyDepartment of Gynecology and Obstetrics, University Medical Center Regensburg, 93053 Regensburg, GermanyChemerin, a pleiotropic adipokine coded by the <i>RARRES2</i> gene, has been reported to affect the pathophysiology of various cancer entities. To further approach the role of this adipokine in ovarian cancer (OC), intratumoral protein levels of chemerin and its receptor chemokine-like receptor 1 (CMKLR1) were examined by immunohistochemistry analyzing tissue microarrays with tumor samples from 208 OC patients. Since chemerin has been reported to affect the female reproductive system, associations with proteins involved in steroid hormone signaling were analyzed. Additionally, correlations with ovarian cancer markers, cancer-related proteins, and survival of OC patients were examined. A positive correlation of chemerin and CMKLR1 protein levels in OC (Spearman’s rho = 0.6, <i>p</i> < 0.0001) was observed. Chemerin staining intensity was strongly associated with the expression of progesterone receptor (PR) (Spearman´s rho = 0.79, <i>p</i> < 0.0001). Both chemerin and CMKLR1 proteins positively correlated with estrogen receptor β (ERβ) and estrogen-related receptors. Neither chemerin nor the CMKLR1 protein level was associated with the survival of OC patients. At the mRNA level, in silico analysis revealed low <i>RARRES2</i> and high <i>CMKLR1</i> expression associated with longer overall survival. The results of our correlation analyses suggested the previously reported interaction of chemerin and estrogen signaling to be present in OC tissue. Further studies are needed to elucidate to which extent this interaction might affect OC development and progression.https://www.mdpi.com/2075-4418/13/5/944chemerinchemokine-like receptor 1estrogen-related receptorsovarian canceroverall survivalprogression-free survival |
spellingShingle | Florian Weber Susanne Schueler-Toprak Christa Buechler Olaf Ortmann Oliver Treeck Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling Diagnostics chemerin chemokine-like receptor 1 estrogen-related receptors ovarian cancer overall survival progression-free survival |
title | Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling |
title_full | Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling |
title_fullStr | Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling |
title_full_unstemmed | Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling |
title_short | Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling |
title_sort | chemerin and chemokine like receptor 1 expression in ovarian cancer associates with proteins involved in estrogen signaling |
topic | chemerin chemokine-like receptor 1 estrogen-related receptors ovarian cancer overall survival progression-free survival |
url | https://www.mdpi.com/2075-4418/13/5/944 |
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