Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive,...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/9/8/218 |
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| author | Francesco Arcadio Luigi Zeni Chiara Perri Girolamo D’Agostino Giudo Chiaretti Giovanni Porto Aldo Minardo Nunzio Cennamo |
| author_facet | Francesco Arcadio Luigi Zeni Chiara Perri Girolamo D’Agostino Giudo Chiaretti Giovanni Porto Aldo Minardo Nunzio Cennamo |
| author_sort | Francesco Arcadio |
| collection | DOAJ |
| description | Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10<sup>−9</sup> M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips. |
| first_indexed | 2024-03-10T08:54:37Z |
| format | Article |
| id | doaj.art-787c8a85761443698971bb1005ed7407 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T08:54:37Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-787c8a85761443698971bb1005ed74072023-11-22T07:13:10ZengMDPI AGChemosensors2227-90402021-08-019821810.3390/chemosensors9080218Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP ReceptorFrancesco Arcadio0Luigi Zeni1Chiara Perri2Girolamo D’Agostino3Giudo Chiaretti4Giovanni Porto5Aldo Minardo6Nunzio Cennamo7Department of Engineering, University of Campania Luigi Vanvitelli, Via Roma n.29, 81031 Aversa, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma n.29, 81031 Aversa, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma n.29, 81031 Aversa, ItalyMoresense Srl, Filarete Foundation, Viale Ortles 22/4, 20139 Milan, ItalyMoresense Srl, Filarete Foundation, Viale Ortles 22/4, 20139 Milan, ItalyMoresense Srl, Filarete Foundation, Viale Ortles 22/4, 20139 Milan, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma n.29, 81031 Aversa, ItalyDepartment of Engineering, University of Campania Luigi Vanvitelli, Via Roma n.29, 81031 Aversa, ItalyNowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10<sup>−9</sup> M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.https://www.mdpi.com/2227-9040/9/8/218surface plasmon resonance (SPR)molecularly imprinted polymers (MIPs)optical sensorsbovine serum albumin (BSA) protein |
| spellingShingle | Francesco Arcadio Luigi Zeni Chiara Perri Girolamo D’Agostino Giudo Chiaretti Giovanni Porto Aldo Minardo Nunzio Cennamo Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor Chemosensors surface plasmon resonance (SPR) molecularly imprinted polymers (MIPs) optical sensors bovine serum albumin (BSA) protein |
| title | Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor |
| title_full | Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor |
| title_fullStr | Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor |
| title_full_unstemmed | Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor |
| title_short | Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor |
| title_sort | bovine serum albumin protein detection by a removable spr chip combined with a specific mip receptor |
| topic | surface plasmon resonance (SPR) molecularly imprinted polymers (MIPs) optical sensors bovine serum albumin (BSA) protein |
| url | https://www.mdpi.com/2227-9040/9/8/218 |
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