Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats
Background/Aims: Previous studies imply that telocytes may have a protective effect on fibrosis in various organs, including the liver, colon, and heart. The effect of telocytes on renal fibrosis remains unknown. Herein, this study was designed to investigate the effect of telocytes on renal fibrosi...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2018-04-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/489445 |
| _version_ | 1828821996369084416 |
|---|---|
| author | Long Zheng Long Li Guisheng Qi Mushuang Hu Chao Hu Shuo Wang Jiawei Li Mingnan Zhang Weitao Zhang Yigang Zeng Yi Zhang Liping Li Xuanchuan Wang Miao Lin Tongyu Zhu Ruiming Rong |
| author_facet | Long Zheng Long Li Guisheng Qi Mushuang Hu Chao Hu Shuo Wang Jiawei Li Mingnan Zhang Weitao Zhang Yigang Zeng Yi Zhang Liping Li Xuanchuan Wang Miao Lin Tongyu Zhu Ruiming Rong |
| author_sort | Long Zheng |
| collection | DOAJ |
| description | Background/Aims: Previous studies imply that telocytes may have a protective effect on fibrosis in various organs, including the liver, colon, and heart. The effect of telocytes on renal fibrosis remains unknown. Herein, this study was designed to investigate the effect of telocytes on renal fibrosis and the potential mechanisms involved. Methods: In a unilateral ureteral obstruction (UUO)-induced renal fibrosis model, telocytes were injected via the tail vein every other day for 10 days. The degree of renal damage and fibrosis was determined using histological assessment. The expression of collagen I, fibronectin, epithelial-mesenchymal transition markers, and Smad2/3 phosphorylation was examined by western blot analyses. Real-time PCR and enzyme-linked immunosorbent assay were performed in vivo to detect the levels of transforming growth factor (TGF)-β1 and various growth factors. Results: Telocytes attenuated renal fibrosis, as evidenced by reduced interstitial collagen accumulation, decreased expression of fibronectin and collagen I, upregulation of E-cadherin, and downregulation of α-smooth muscle actin. Furthermore, telocytes decreased serum TGF-β1 levels, suppressed Smad2/3 phosphorylation, and increased the expression of hepatocyte growth factor (HGF) in rat kidney tissue following UUO. Blockage of HGF counteracted the protective effect of telocytes on UUO-treated kidneys. Through the detection of HGF mRNA levels in vitro, we found that telocytes had no effect on HGF expression compared with renal fibroblasts. Conclusion: Telocytes attenuated UUO-induced renal fibrosis in rats, likely through enhancing the expression of HGF in an indirect manner. |
| first_indexed | 2024-12-12T13:05:36Z |
| format | Article |
| id | doaj.art-787d5dd3618d492d9f0d432152b731dd |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-12T13:05:36Z |
| publishDate | 2018-04-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-787d5dd3618d492d9f0d432152b731dd2022-12-22T00:23:40ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-04-014652056207110.1159/000489445489445Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in RatsLong ZhengLong LiGuisheng QiMushuang HuChao HuShuo WangJiawei LiMingnan ZhangWeitao ZhangYigang ZengYi ZhangLiping LiXuanchuan WangMiao LinTongyu ZhuRuiming RongBackground/Aims: Previous studies imply that telocytes may have a protective effect on fibrosis in various organs, including the liver, colon, and heart. The effect of telocytes on renal fibrosis remains unknown. Herein, this study was designed to investigate the effect of telocytes on renal fibrosis and the potential mechanisms involved. Methods: In a unilateral ureteral obstruction (UUO)-induced renal fibrosis model, telocytes were injected via the tail vein every other day for 10 days. The degree of renal damage and fibrosis was determined using histological assessment. The expression of collagen I, fibronectin, epithelial-mesenchymal transition markers, and Smad2/3 phosphorylation was examined by western blot analyses. Real-time PCR and enzyme-linked immunosorbent assay were performed in vivo to detect the levels of transforming growth factor (TGF)-β1 and various growth factors. Results: Telocytes attenuated renal fibrosis, as evidenced by reduced interstitial collagen accumulation, decreased expression of fibronectin and collagen I, upregulation of E-cadherin, and downregulation of α-smooth muscle actin. Furthermore, telocytes decreased serum TGF-β1 levels, suppressed Smad2/3 phosphorylation, and increased the expression of hepatocyte growth factor (HGF) in rat kidney tissue following UUO. Blockage of HGF counteracted the protective effect of telocytes on UUO-treated kidneys. Through the detection of HGF mRNA levels in vitro, we found that telocytes had no effect on HGF expression compared with renal fibroblasts. Conclusion: Telocytes attenuated UUO-induced renal fibrosis in rats, likely through enhancing the expression of HGF in an indirect manner.https://www.karger.com/Article/FullText/489445TelocyteRenal fibrosisUUOHGF |
| spellingShingle | Long Zheng Long Li Guisheng Qi Mushuang Hu Chao Hu Shuo Wang Jiawei Li Mingnan Zhang Weitao Zhang Yigang Zeng Yi Zhang Liping Li Xuanchuan Wang Miao Lin Tongyu Zhu Ruiming Rong Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats Cellular Physiology and Biochemistry Telocyte Renal fibrosis UUO HGF |
| title | Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats |
| title_full | Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats |
| title_fullStr | Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats |
| title_full_unstemmed | Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats |
| title_short | Transplantation of Telocytes Attenuates Unilateral Ureter Obstruction-Induced Renal Fibrosis in Rats |
| title_sort | transplantation of telocytes attenuates unilateral ureter obstruction induced renal fibrosis in rats |
| topic | Telocyte Renal fibrosis UUO HGF |
| url | https://www.karger.com/Article/FullText/489445 |
| work_keys_str_mv | AT longzheng transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT longli transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT guishengqi transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT mushuanghu transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT chaohu transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT shuowang transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT jiaweili transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT mingnanzhang transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT weitaozhang transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT yigangzeng transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT yizhang transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT lipingli transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT xuanchuanwang transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT miaolin transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT tongyuzhu transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats AT ruimingrong transplantationoftelocytesattenuatesunilateralureterobstructioninducedrenalfibrosisinrats |