Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling
AbstractBackground:Diet-induced obesity is frequently used to demonstrate cardiac dysfunction. However, some rats, like humans, are susceptible to developing an obesity phenotype, whereas others are resistant to that.Objective:To evaluate the association between obesity resistance and cardiac functi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Cardiologia (SBC)
2015-01-01
|
| Series: | Arquivos Brasileiros de Cardiologia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2015005050134&lng=en&tlng=en |
| _version_ | 1819116174192934912 |
|---|---|
| author | Felipe Gonçalves dos Santos de Sá Ana Paula Lima-Leopoldo Bruno Barcellos Jacobsen Artur Junio Togneri Ferron Wagner Muller Estevam Dijon Henrique Salomé Campos Edson Castardeli Márcia Regina Holanda da Cunha Antonio Carlos Cicogna André Soares Leopoldo |
| author_facet | Felipe Gonçalves dos Santos de Sá Ana Paula Lima-Leopoldo Bruno Barcellos Jacobsen Artur Junio Togneri Ferron Wagner Muller Estevam Dijon Henrique Salomé Campos Edson Castardeli Márcia Regina Holanda da Cunha Antonio Carlos Cicogna André Soares Leopoldo |
| author_sort | Felipe Gonçalves dos Santos de Sá |
| collection | DOAJ |
| description | AbstractBackground:Diet-induced obesity is frequently used to demonstrate cardiac dysfunction. However, some rats, like humans, are susceptible to developing an obesity phenotype, whereas others are resistant to that.Objective:To evaluate the association between obesity resistance and cardiac function, and the impact of obesity resistance on calcium handling.Methods:Thirty-day-old male Wistar rats were distributed into two groups, each with 54 animals: control (C; standard diet) and obese (four palatable high-fat diets) for 15 weeks. After the experimental protocol, rats consuming the high-fat diets were classified according to the adiposity index and subdivided into obesity-prone (OP) and obesity-resistant (OR). Nutritional profile, comorbidities, and cardiac remodeling were evaluated. Cardiac function was assessed by papillary muscle evaluation at baseline and after inotropic maneuvers.Results:The high-fat diets promoted increase in body fat and adiposity index in OP rats compared with C and OR rats. Glucose, lipid, and blood pressure profiles remained unchanged in OR rats. In addition, the total heart weight and the weight of the left and right ventricles in OR rats were lower than those in OP rats, but similar to those in C rats. Baseline cardiac muscle data were similar in all rats, but myocardial responsiveness to a post-rest contraction stimulus was compromised in OP and OR rats compared with C rats.Conclusion:Obesity resistance promoted specific changes in the contraction phase without changes in the relaxation phase. This mild abnormality may be related to intracellular Ca2+ handling. |
| first_indexed | 2024-12-22T05:12:53Z |
| format | Article |
| id | doaj.art-7880be1f6c614a59b836d051bf6f89c8 |
| institution | Directory Open Access Journal |
| issn | 1678-4170 |
| language | English |
| last_indexed | 2024-12-22T05:12:53Z |
| publishDate | 2015-01-01 |
| publisher | Sociedade Brasileira de Cardiologia (SBC) |
| record_format | Article |
| series | Arquivos Brasileiros de Cardiologia |
| spelling | doaj.art-7880be1f6c614a59b836d051bf6f89c82022-12-21T18:37:56ZengSociedade Brasileira de Cardiologia (SBC)Arquivos Brasileiros de Cardiologia1678-41702015-01-0100010.5935/abc.20150134S0066-782X2015005050134Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ HandlingFelipe Gonçalves dos Santos de SáAna Paula Lima-LeopoldoBruno Barcellos JacobsenArtur Junio Togneri FerronWagner Muller EstevamDijon Henrique Salomé CamposEdson CastardeliMárcia Regina Holanda da CunhaAntonio Carlos CicognaAndré Soares LeopoldoAbstractBackground:Diet-induced obesity is frequently used to demonstrate cardiac dysfunction. However, some rats, like humans, are susceptible to developing an obesity phenotype, whereas others are resistant to that.Objective:To evaluate the association between obesity resistance and cardiac function, and the impact of obesity resistance on calcium handling.Methods:Thirty-day-old male Wistar rats were distributed into two groups, each with 54 animals: control (C; standard diet) and obese (four palatable high-fat diets) for 15 weeks. After the experimental protocol, rats consuming the high-fat diets were classified according to the adiposity index and subdivided into obesity-prone (OP) and obesity-resistant (OR). Nutritional profile, comorbidities, and cardiac remodeling were evaluated. Cardiac function was assessed by papillary muscle evaluation at baseline and after inotropic maneuvers.Results:The high-fat diets promoted increase in body fat and adiposity index in OP rats compared with C and OR rats. Glucose, lipid, and blood pressure profiles remained unchanged in OR rats. In addition, the total heart weight and the weight of the left and right ventricles in OR rats were lower than those in OP rats, but similar to those in C rats. Baseline cardiac muscle data were similar in all rats, but myocardial responsiveness to a post-rest contraction stimulus was compromised in OP and OR rats compared with C rats.Conclusion:Obesity resistance promoted specific changes in the contraction phase without changes in the relaxation phase. This mild abnormality may be related to intracellular Ca2+ handling.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2015005050134&lng=en&tlng=enResistência à ObesidadeDieta HiperlipídicaFunção CardíacaTrânsito de CálcioRatos |
| spellingShingle | Felipe Gonçalves dos Santos de Sá Ana Paula Lima-Leopoldo Bruno Barcellos Jacobsen Artur Junio Togneri Ferron Wagner Muller Estevam Dijon Henrique Salomé Campos Edson Castardeli Márcia Regina Holanda da Cunha Antonio Carlos Cicogna André Soares Leopoldo Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling Arquivos Brasileiros de Cardiologia Resistência à Obesidade Dieta Hiperlipídica Função Cardíaca Trânsito de Cálcio Ratos |
| title | Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling |
| title_full | Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling |
| title_fullStr | Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling |
| title_full_unstemmed | Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling |
| title_short | Obesity Resistance Promotes Mild Contractile Dysfunction Associated with Intracellular Ca2+ Handling |
| title_sort | obesity resistance promotes mild contractile dysfunction associated with intracellular ca2 handling |
| topic | Resistência à Obesidade Dieta Hiperlipídica Função Cardíaca Trânsito de Cálcio Ratos |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2015005050134&lng=en&tlng=en |
| work_keys_str_mv | AT felipegoncalvesdossantosdesa obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT anapaulalimaleopoldo obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT brunobarcellosjacobsen obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT arturjuniotogneriferron obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT wagnermullerestevam obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT dijonhenriquesalomecampos obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT edsoncastardeli obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT marciareginaholandadacunha obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT antoniocarloscicogna obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling AT andresoaresleopoldo obesityresistancepromotesmildcontractiledysfunctionassociatedwithintracellularca2handling |