Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women.
<h4>Background</h4>Bacterial vaginosis (BV) is one of the most common urogenital infections among women of reproductive age that represents shifts in microbiota from Lactobacillus spp. to diverse anaerobes. The aim of our study was to evalute the diagnostic values of Gardnerella, Atopobi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24124575/pdf/?tool=EBI |
| _version_ | 1818575470063517696 |
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| author | Zongxin Ling Xia Liu Yueqiu Luo Xiaoxing Wu Li Yuan Xiaojuan Tong Lanjuan Li Charlie Xiang |
| author_facet | Zongxin Ling Xia Liu Yueqiu Luo Xiaoxing Wu Li Yuan Xiaojuan Tong Lanjuan Li Charlie Xiang |
| author_sort | Zongxin Ling |
| collection | DOAJ |
| description | <h4>Background</h4>Bacterial vaginosis (BV) is one of the most common urogenital infections among women of reproductive age that represents shifts in microbiota from Lactobacillus spp. to diverse anaerobes. The aim of our study was to evalute the diagnostic values of Gardnerella, Atopobium, Eggerthella, Megasphaera typeI, Leptotrichia/Sneathia and Prevotella, defined as a vaginal pathogenic community for BV and their associations with vaginal pH and Nugent scores.<h4>Methods and findings</h4>We investigated the vaginal pathogenic bacteria and Lactobacillus spp. with species-specific real-time quantitative PCR (qPCR) in 50 BV-positive and 50 BV-negative Chinese women of reproductive age. Relative to BV-negative subjects, a siginificant decline in Lactobacillus and an obvious increase in bacteria in the vaginal pathogenic community were observed in BV-postive subjects (P<0.05). With the exception of Megasphaera typeI, other vaginal pathogenic bacteria were highly predictable for BV with a better sensitivity and specificity. The vaginal pathogenic community was positively associated with vaginal pH and Nugent scores, while Lactobacillus spp., such as L. iners and L. crispatus was negatively associated with them (P<0.05).<h4>Conclusions</h4>Our data implied that the prevalance of vaginal pathogenic bacteria as well as the depletion of Lactobacillus was highly accurate for BV diagnosis. Vaginal microbiota shifts, especially the overgrowth of the vaginal pathogenic community, showed well diagnostic values in predicting BV. Postive correlations between those vaginal pathogenic bacteria and vaginal pH, Nugent score indicated the vaginal pathogenic community rather than a single vaginal microorganism, was participated in the onset of BV directly. |
| first_indexed | 2024-12-15T00:40:43Z |
| format | Article |
| id | doaj.art-788380c95da3469c9659a82d89b09657 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-15T00:40:43Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-788380c95da3469c9659a82d89b096572022-12-21T22:41:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7658910.1371/journal.pone.0076589Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women.Zongxin LingXia LiuYueqiu LuoXiaoxing WuLi YuanXiaojuan TongLanjuan LiCharlie Xiang<h4>Background</h4>Bacterial vaginosis (BV) is one of the most common urogenital infections among women of reproductive age that represents shifts in microbiota from Lactobacillus spp. to diverse anaerobes. The aim of our study was to evalute the diagnostic values of Gardnerella, Atopobium, Eggerthella, Megasphaera typeI, Leptotrichia/Sneathia and Prevotella, defined as a vaginal pathogenic community for BV and their associations with vaginal pH and Nugent scores.<h4>Methods and findings</h4>We investigated the vaginal pathogenic bacteria and Lactobacillus spp. with species-specific real-time quantitative PCR (qPCR) in 50 BV-positive and 50 BV-negative Chinese women of reproductive age. Relative to BV-negative subjects, a siginificant decline in Lactobacillus and an obvious increase in bacteria in the vaginal pathogenic community were observed in BV-postive subjects (P<0.05). With the exception of Megasphaera typeI, other vaginal pathogenic bacteria were highly predictable for BV with a better sensitivity and specificity. The vaginal pathogenic community was positively associated with vaginal pH and Nugent scores, while Lactobacillus spp., such as L. iners and L. crispatus was negatively associated with them (P<0.05).<h4>Conclusions</h4>Our data implied that the prevalance of vaginal pathogenic bacteria as well as the depletion of Lactobacillus was highly accurate for BV diagnosis. Vaginal microbiota shifts, especially the overgrowth of the vaginal pathogenic community, showed well diagnostic values in predicting BV. Postive correlations between those vaginal pathogenic bacteria and vaginal pH, Nugent score indicated the vaginal pathogenic community rather than a single vaginal microorganism, was participated in the onset of BV directly.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24124575/pdf/?tool=EBI |
| spellingShingle | Zongxin Ling Xia Liu Yueqiu Luo Xiaoxing Wu Li Yuan Xiaojuan Tong Lanjuan Li Charlie Xiang Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. PLoS ONE |
| title | Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. |
| title_full | Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. |
| title_fullStr | Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. |
| title_full_unstemmed | Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. |
| title_short | Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. |
| title_sort | associations between vaginal pathogenic community and bacterial vaginosis in chinese reproductive age women |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24124575/pdf/?tool=EBI |
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