Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells
Glioblastoma multiforme (GBM) is a rare, yet devastating, primary brain tumor in adults. Current treatments remain generally ineffective and GBM almost invariably recurs, resulting in median survival of 15 months. This high malignancy sources notably from the resilience and invasive capabilities of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.981583/full |
| _version_ | 1828105195731550208 |
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| author | Mathilde Kerhervé Mathilde Kerhervé Sara Rosińska Sara Rosińska Kilian Trillet Kilian Trillet Alya Zeinaty Alya Zeinaty Magalie Feyeux Steven Nedellec Julie Gavard Julie Gavard Julie Gavard |
| author_facet | Mathilde Kerhervé Mathilde Kerhervé Sara Rosińska Sara Rosińska Kilian Trillet Kilian Trillet Alya Zeinaty Alya Zeinaty Magalie Feyeux Steven Nedellec Julie Gavard Julie Gavard Julie Gavard |
| author_sort | Mathilde Kerhervé |
| collection | DOAJ |
| description | Glioblastoma multiforme (GBM) is a rare, yet devastating, primary brain tumor in adults. Current treatments remain generally ineffective and GBM almost invariably recurs, resulting in median survival of 15 months. This high malignancy sources notably from the resilience and invasive capabilities of tumor cells. Within GBM, exists a population of self-sustaining transformed cells with stem-like properties (GSCs), which are thought to be responsible for tumor initiation, growth, and invasion, as well as recurrence. In the tumor microenvironment, GSCs might be found in the vicinity of brain endothelial cells, which provide a protective habitat. Likewise, these resistant, quiescent GSCs may accumulate in hypoxic zones, away from the perivascular niche, or travel towards the healthy brain parenchyma, by eminently co-opting neuro-vascular tracks. Herein, we established an ex vivo model to explore GSC invasive behavior. We found that patient-derived cells massively invade the collagen matrix. In addition, we described that the glycoprotein Neuropilin-1 (NRP1) contributes to GSC spreading and invasion. Indeed, both RNA interference-mediated silencing and CRISPR-mediated gene editing deletion of NRP1 strongly impaired the 3D invasive properties of patient-derived GSCs and their close localization to the brain blood vessels. Of note, other typical features of GSCs, such as expansion and self-renewal were maintained. From a mechanistic standpoint, this biological effect might rely on the expression of the β3 subunit integrin cell-extracellular matrix adhesive receptor. Our data, therefore, propose a reliable approach to explore invasive properties of patient glioma cells ex vivo and identify NRP1 as a mediator in this malignant process. |
| first_indexed | 2024-04-11T09:59:36Z |
| format | Article |
| id | doaj.art-78921b0375514f75b487556af6e1b7cc |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-11T09:59:36Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-78921b0375514f75b487556af6e1b7cc2022-12-22T04:30:27ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-09-011010.3389/fcell.2022.981583981583Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cellsMathilde Kerhervé0Mathilde Kerhervé1Sara Rosińska2Sara Rosińska3Kilian Trillet4Kilian Trillet5Alya Zeinaty6Alya Zeinaty7Magalie Feyeux8Steven Nedellec9Julie Gavard10Julie Gavard11Julie Gavard12Team SOAP, CRCI2NA, Nantes Université, Inserm, CNRS, Université D’Angers, Nantes, FranceEquipe Labellisée Ligue Contre le Cancer, Nantes, FranceTeam SOAP, CRCI2NA, Nantes Université, Inserm, CNRS, Université D’Angers, Nantes, FranceEquipe Labellisée Ligue Contre le Cancer, Nantes, FranceTeam SOAP, CRCI2NA, Nantes Université, Inserm, CNRS, Université D’Angers, Nantes, FranceEquipe Labellisée Ligue Contre le Cancer, Nantes, FranceTeam SOAP, CRCI2NA, Nantes Université, Inserm, CNRS, Université D’Angers, Nantes, FranceEquipe Labellisée Ligue Contre le Cancer, Nantes, FranceNantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, SFR Bonamy, Nantes, FranceNantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, SFR Bonamy, Nantes, FranceTeam SOAP, CRCI2NA, Nantes Université, Inserm, CNRS, Université D’Angers, Nantes, FranceEquipe Labellisée Ligue Contre le Cancer, Nantes, FranceInstitut de Cancérologie de L’Ouest (ICO), Angers, FranceGlioblastoma multiforme (GBM) is a rare, yet devastating, primary brain tumor in adults. Current treatments remain generally ineffective and GBM almost invariably recurs, resulting in median survival of 15 months. This high malignancy sources notably from the resilience and invasive capabilities of tumor cells. Within GBM, exists a population of self-sustaining transformed cells with stem-like properties (GSCs), which are thought to be responsible for tumor initiation, growth, and invasion, as well as recurrence. In the tumor microenvironment, GSCs might be found in the vicinity of brain endothelial cells, which provide a protective habitat. Likewise, these resistant, quiescent GSCs may accumulate in hypoxic zones, away from the perivascular niche, or travel towards the healthy brain parenchyma, by eminently co-opting neuro-vascular tracks. Herein, we established an ex vivo model to explore GSC invasive behavior. We found that patient-derived cells massively invade the collagen matrix. In addition, we described that the glycoprotein Neuropilin-1 (NRP1) contributes to GSC spreading and invasion. Indeed, both RNA interference-mediated silencing and CRISPR-mediated gene editing deletion of NRP1 strongly impaired the 3D invasive properties of patient-derived GSCs and their close localization to the brain blood vessels. Of note, other typical features of GSCs, such as expansion and self-renewal were maintained. From a mechanistic standpoint, this biological effect might rely on the expression of the β3 subunit integrin cell-extracellular matrix adhesive receptor. Our data, therefore, propose a reliable approach to explore invasive properties of patient glioma cells ex vivo and identify NRP1 as a mediator in this malignant process.https://www.frontiersin.org/articles/10.3389/fcell.2022.981583/fulladhesioncollagengliomaintegrininvasionmigration |
| spellingShingle | Mathilde Kerhervé Mathilde Kerhervé Sara Rosińska Sara Rosińska Kilian Trillet Kilian Trillet Alya Zeinaty Alya Zeinaty Magalie Feyeux Steven Nedellec Julie Gavard Julie Gavard Julie Gavard Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells Frontiers in Cell and Developmental Biology adhesion collagen glioma integrin invasion migration |
| title | Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells |
| title_full | Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells |
| title_fullStr | Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells |
| title_full_unstemmed | Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells |
| title_short | Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells |
| title_sort | neuropilin 1 modulates the 3d invasive properties of glioblastoma stem like cells |
| topic | adhesion collagen glioma integrin invasion migration |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.981583/full |
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