Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics
<i>Candida albicans</i> is a typical opportunistic pathogen in humans that causes serious health risks in clinical fungal infections. The construction of mutant libraries has made remarkable developments in the study of <i>C. albicans</i> molecular and cellular biology with t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12307 |
| _version_ | 1797440886001369088 |
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| author | Ruina Wang Jiacun Liu Yu Liu Quanzhen Lv Lan Yan |
| author_facet | Ruina Wang Jiacun Liu Yu Liu Quanzhen Lv Lan Yan |
| author_sort | Ruina Wang |
| collection | DOAJ |
| description | <i>Candida albicans</i> is a typical opportunistic pathogen in humans that causes serious health risks in clinical fungal infections. The construction of mutant libraries has made remarkable developments in the study of <i>C. albicans</i> molecular and cellular biology with the ongoing advancements of gene editing, which include the application of CRISPR-Cas9 and novel high-efficient transposon. Large-scale genetic screens and genome-wide functional analysis accelerated the investigation of new genetic regulatory mechanisms associated with the pathogenicity and resistance to environmental stress in <i>C. albicans</i>. More importantly, sensitivity screening based on <i>C. albicans</i> mutant libraries is critical for the target identification of novel antifungal compounds, which leads to the discovery of Sec7p, Tfp1p, Gwt1p, Gln4p, and Erg11p. This review summarizes the main types of <i>C. albicans</i> mutant libraries and interprets their applications in morphogenesis, biofilm formation, fungus–host interactions, antifungal drug resistance, and target identification. |
| first_indexed | 2024-03-09T12:15:53Z |
| format | Article |
| id | doaj.art-789293627bfb46318bfaa7bb619cfc24 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:15:53Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-789293627bfb46318bfaa7bb619cfc242023-11-30T22:47:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201230710.3390/ijms232012307Application of the Mutant Libraries for <i>Candida albicans</i> Functional GenomicsRuina Wang0Jiacun Liu1Yu Liu2Quanzhen Lv3Lan Yan4School of Pharmacy, Naval Medical University, 325 Guohe Road, Shanghai 200433, ChinaSchool of Pharmacy, Naval Medical University, 325 Guohe Road, Shanghai 200433, ChinaSchool of Pharmacy, Naval Medical University, 325 Guohe Road, Shanghai 200433, ChinaSchool of Pharmacy, Naval Medical University, 325 Guohe Road, Shanghai 200433, ChinaSchool of Pharmacy, Naval Medical University, 325 Guohe Road, Shanghai 200433, China<i>Candida albicans</i> is a typical opportunistic pathogen in humans that causes serious health risks in clinical fungal infections. The construction of mutant libraries has made remarkable developments in the study of <i>C. albicans</i> molecular and cellular biology with the ongoing advancements of gene editing, which include the application of CRISPR-Cas9 and novel high-efficient transposon. Large-scale genetic screens and genome-wide functional analysis accelerated the investigation of new genetic regulatory mechanisms associated with the pathogenicity and resistance to environmental stress in <i>C. albicans</i>. More importantly, sensitivity screening based on <i>C. albicans</i> mutant libraries is critical for the target identification of novel antifungal compounds, which leads to the discovery of Sec7p, Tfp1p, Gwt1p, Gln4p, and Erg11p. This review summarizes the main types of <i>C. albicans</i> mutant libraries and interprets their applications in morphogenesis, biofilm formation, fungus–host interactions, antifungal drug resistance, and target identification.https://www.mdpi.com/1422-0067/23/20/12307<i>Candida albicans</i>mutant libraryfunctional genomicsphenotypevirulencegenetic screening |
| spellingShingle | Ruina Wang Jiacun Liu Yu Liu Quanzhen Lv Lan Yan Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics International Journal of Molecular Sciences <i>Candida albicans</i> mutant library functional genomics phenotype virulence genetic screening |
| title | Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics |
| title_full | Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics |
| title_fullStr | Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics |
| title_full_unstemmed | Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics |
| title_short | Application of the Mutant Libraries for <i>Candida albicans</i> Functional Genomics |
| title_sort | application of the mutant libraries for i candida albicans i functional genomics |
| topic | <i>Candida albicans</i> mutant library functional genomics phenotype virulence genetic screening |
| url | https://www.mdpi.com/1422-0067/23/20/12307 |
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