Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles
Gold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NC...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/21/3875 |
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| author | Regina M. Chiechio Solène Ducarre Célia Marets Aurélien Dupont Pascale Even-Hernandez Xavier Pinson Stéphanie Dutertre Franck Artzner Paolo Musumeci Célia Ravel Maria Jose Lo Faro Valérie Marchi |
| author_facet | Regina M. Chiechio Solène Ducarre Célia Marets Aurélien Dupont Pascale Even-Hernandez Xavier Pinson Stéphanie Dutertre Franck Artzner Paolo Musumeci Célia Ravel Maria Jose Lo Faro Valérie Marchi |
| author_sort | Regina M. Chiechio |
| collection | DOAJ |
| description | Gold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NCs into liposomes of various sizes and chemical compositions. Different methods were investigated to prepare vesicles containing Au NCs in their lumen. The efficiency of the process was correlated to the structural and morphological aspect of the Au NCs’ encapsulating vesicles thanks to complementary analyses by SAXS, cryo-TEM, and confocal microscopy techniques. Cell-like-sized vesicles (GUVs) encapsulating red or blue Au NCs were successfully obtained by an innovative method using emulsion phase transfer. Furthermore, exosome-like-sized vesicles (LUVs) containing Au NCs were obtained with an encapsulation yield of 40%, as estimated from ICP-MS. |
| first_indexed | 2024-03-09T18:47:02Z |
| format | Article |
| id | doaj.art-789841c00d284ecf906fd7d1f62d35e0 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T18:47:02Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-789841c00d284ecf906fd7d1f62d35e02023-11-24T06:10:37ZengMDPI AGNanomaterials2079-49912022-11-011221387510.3390/nano12213875Encapsulation of Luminescent Gold Nanoclusters into Synthetic VesiclesRegina M. Chiechio0Solène Ducarre1Célia Marets2Aurélien Dupont3Pascale Even-Hernandez4Xavier Pinson5Stéphanie Dutertre6Franck Artzner7Paolo Musumeci8Célia Ravel9Maria Jose Lo Faro10Valérie Marchi11Institut des Sciences Chimiques de Rennes, CNRS UMR 6226, Université Rennes 1, F-35000 Rennes, FranceIMM-CNR, Via S. Sofia 64, 95123 Catania, ItalyInstitut des Sciences Chimiques de Rennes, CNRS UMR 6226, Université Rennes 1, F-35000 Rennes, FranceBIOSIT, Inserm, CNRS UMS 3480, Université Rennes1, US_S 018, F-35000 Rennes, FranceInstitut des Sciences Chimiques de Rennes, CNRS UMR 6226, Université Rennes 1, F-35000 Rennes, FranceMicroscopy Rennes Imaging Centre, SFR Biosit, CNRS UMS 3480—US INSERM 018, Université Rennes 1, F-35000 Rennes, FranceMicroscopy Rennes Imaging Centre, SFR Biosit, CNRS UMS 3480—US INSERM 018, Université Rennes 1, F-35000 Rennes, FranceInstitut de Physique, CNRS UMR 6251, Université Rennes 1, F-35000 Rennes, FranceDipartimento di Fisica e Astronomia “Ettore Majorana”, Università Di Catania, Via Santa Sofia 64, 95123 Catania, ItalyService de Biologie de la Reproduction-CECOS, CHU Rennes, F-35000 Rennes, FranceDipartimento di Fisica e Astronomia “Ettore Majorana”, Università Di Catania, Via Santa Sofia 64, 95123 Catania, ItalyInstitut des Sciences Chimiques de Rennes, CNRS UMR 6226, Université Rennes 1, F-35000 Rennes, FranceGold nanoclusters (Au NCs) are attractive luminescent nanoprobes for biomedical applications. In vivo biosensing and bioimaging requires the delivery of the Au NCs into subcellular compartments. In this view, we explore here the possible encapsulation of ultra-small-sized red and blue emitting Au NCs into liposomes of various sizes and chemical compositions. Different methods were investigated to prepare vesicles containing Au NCs in their lumen. The efficiency of the process was correlated to the structural and morphological aspect of the Au NCs’ encapsulating vesicles thanks to complementary analyses by SAXS, cryo-TEM, and confocal microscopy techniques. Cell-like-sized vesicles (GUVs) encapsulating red or blue Au NCs were successfully obtained by an innovative method using emulsion phase transfer. Furthermore, exosome-like-sized vesicles (LUVs) containing Au NCs were obtained with an encapsulation yield of 40%, as estimated from ICP-MS.https://www.mdpi.com/2079-4991/12/21/3875gold nanoclustersvesiclesencapsulation |
| spellingShingle | Regina M. Chiechio Solène Ducarre Célia Marets Aurélien Dupont Pascale Even-Hernandez Xavier Pinson Stéphanie Dutertre Franck Artzner Paolo Musumeci Célia Ravel Maria Jose Lo Faro Valérie Marchi Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles Nanomaterials gold nanoclusters vesicles encapsulation |
| title | Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles |
| title_full | Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles |
| title_fullStr | Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles |
| title_full_unstemmed | Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles |
| title_short | Encapsulation of Luminescent Gold Nanoclusters into Synthetic Vesicles |
| title_sort | encapsulation of luminescent gold nanoclusters into synthetic vesicles |
| topic | gold nanoclusters vesicles encapsulation |
| url | https://www.mdpi.com/2079-4991/12/21/3875 |
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