A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we hav...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/15/2360 |
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| author | Haiyan Zheng Yu Pei Yuan-Lin He Yi Liu Minqi Chen Pengzhi Hong Chunxia Zhou Zhong-Ji Qian |
| author_facet | Haiyan Zheng Yu Pei Yuan-Lin He Yi Liu Minqi Chen Pengzhi Hong Chunxia Zhou Zhong-Ji Qian |
| author_sort | Haiyan Zheng |
| collection | DOAJ |
| description | Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we have investigated the influence and mechanism of GNP (sulfated polysaccharide from <i>Gelidium crinale</i>) on tumor metastasis and MMP-9 expression of human fibrosarcoma (HT1080) cells. The results inflected that the concentration of GNP below 100 μg/mL has no toxicity to HT1080 cells, but showed excellent activity in inhibiting cells migration and invasion. In addition, GNP effectively inhibits the mRNA of MMP-9 and reduces its expression and activity by regulating nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK) and mTOR/PI3K/Akt signaling pathways. GNP has great potential as MMP-9 inhibitor and could be developed as a functional food or drug to prevent tumor metastasis. |
| first_indexed | 2024-03-09T12:36:59Z |
| format | Article |
| id | doaj.art-78990a6e145d4e19b6370d389a567d8d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:36:59Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-78990a6e145d4e19b6370d389a567d8d2023-11-30T22:23:16ZengMDPI AGFoods2304-81582022-08-011115236010.3390/foods11152360A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 CellsHaiyan Zheng0Yu Pei1Yuan-Lin He2Yi Liu3Minqi Chen4Pengzhi Hong5Chunxia Zhou6Zhong-Ji Qian7Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, ChinaSulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we have investigated the influence and mechanism of GNP (sulfated polysaccharide from <i>Gelidium crinale</i>) on tumor metastasis and MMP-9 expression of human fibrosarcoma (HT1080) cells. The results inflected that the concentration of GNP below 100 μg/mL has no toxicity to HT1080 cells, but showed excellent activity in inhibiting cells migration and invasion. In addition, GNP effectively inhibits the mRNA of MMP-9 and reduces its expression and activity by regulating nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK) and mTOR/PI3K/Akt signaling pathways. GNP has great potential as MMP-9 inhibitor and could be developed as a functional food or drug to prevent tumor metastasis.https://www.mdpi.com/2304-8158/11/15/2360sulfated polysaccharides<i>Gelidium crinale</i>MMP-9migrationinvasion |
| spellingShingle | Haiyan Zheng Yu Pei Yuan-Lin He Yi Liu Minqi Chen Pengzhi Hong Chunxia Zhou Zhong-Ji Qian A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells Foods sulfated polysaccharides <i>Gelidium crinale</i> MMP-9 migration invasion |
| title | A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells |
| title_full | A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells |
| title_fullStr | A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells |
| title_full_unstemmed | A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells |
| title_short | A Sulfated Polysaccharide from Red Algae (<i>Gelidium crinale</i>) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells |
| title_sort | sulfated polysaccharide from red algae i gelidium crinale i to suppress cells metastasis and mmp 9 expression of ht1080 cells |
| topic | sulfated polysaccharides <i>Gelidium crinale</i> MMP-9 migration invasion |
| url | https://www.mdpi.com/2304-8158/11/15/2360 |
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