Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles
Synthetic bioactive aromatic peptide amphiphiles have been recognized as key elements of emerging biomedical strategies due to their biocompatibility, design flexibility, and functionality. Inspired by natural proteins, we synthesized two supramolecular materials of phenyl-capped Ile-Lys-Val-Ala-Val...
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4115 |
| _version_ | 1797434090060775424 |
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| author | Fang-Yi Wu Hsin-Chieh Lin |
| author_facet | Fang-Yi Wu Hsin-Chieh Lin |
| author_sort | Fang-Yi Wu |
| collection | DOAJ |
| description | Synthetic bioactive aromatic peptide amphiphiles have been recognized as key elements of emerging biomedical strategies due to their biocompatibility, design flexibility, and functionality. Inspired by natural proteins, we synthesized two supramolecular materials of phenyl-capped Ile-Lys-Val-Ala-Val (<b>Ben-IKVAV</b>) and perfluorophenyl-capped Ile-Lys-Val-Ala-Val (<b>PFB-IKVAV</b>). We employed UV-vis absorption, fluorescence, circular dichroism, and Fourier-transform infrared spectroscopy to examine the driving force in the self-assembly of the newly discovered materials. It was found that both compounds exhibited ordered π-π interactions and secondary structures, especially <b>PFB-IKVAV</b>. The cytotoxicity of human mesenchymal stem cells (hMSCs) and cell differentiation studies was also performed. In addition, the immunofluorescent staining for neuronal-specific markers of MAP2 was 4.6 times (neural induction medium in the presence of <b>PFB-IKVAV</b>) that of the neural induction medium (control) on day 7. From analyzing the expression of neuronal-specific markers in hMSCs, it can be concluded that <b>PFB-IKVAV</b> may be a potential supramolecular biomaterial for biomedical applications. |
| first_indexed | 2024-03-09T10:26:29Z |
| format | Article |
| id | doaj.art-789b41f0adda49a69b8b2b36e3b858b4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:26:29Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-789b41f0adda49a69b8b2b36e3b858b42023-12-01T21:36:07ZengMDPI AGMolecules1420-30492022-06-012713411510.3390/molecules27134115Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide AmphiphilesFang-Yi Wu0Hsin-Chieh Lin1Department of Materials Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu 300093, Taiwan, ChinaDepartment of Materials Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu 300093, Taiwan, ChinaSynthetic bioactive aromatic peptide amphiphiles have been recognized as key elements of emerging biomedical strategies due to their biocompatibility, design flexibility, and functionality. Inspired by natural proteins, we synthesized two supramolecular materials of phenyl-capped Ile-Lys-Val-Ala-Val (<b>Ben-IKVAV</b>) and perfluorophenyl-capped Ile-Lys-Val-Ala-Val (<b>PFB-IKVAV</b>). We employed UV-vis absorption, fluorescence, circular dichroism, and Fourier-transform infrared spectroscopy to examine the driving force in the self-assembly of the newly discovered materials. It was found that both compounds exhibited ordered π-π interactions and secondary structures, especially <b>PFB-IKVAV</b>. The cytotoxicity of human mesenchymal stem cells (hMSCs) and cell differentiation studies was also performed. In addition, the immunofluorescent staining for neuronal-specific markers of MAP2 was 4.6 times (neural induction medium in the presence of <b>PFB-IKVAV</b>) that of the neural induction medium (control) on day 7. From analyzing the expression of neuronal-specific markers in hMSCs, it can be concluded that <b>PFB-IKVAV</b> may be a potential supramolecular biomaterial for biomedical applications.https://www.mdpi.com/1420-3049/27/13/4115self-assemblypeptideamphiphilehydrogelbiomaterial |
| spellingShingle | Fang-Yi Wu Hsin-Chieh Lin Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles Molecules self-assembly peptide amphiphile hydrogel biomaterial |
| title | Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles |
| title_full | Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles |
| title_fullStr | Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles |
| title_full_unstemmed | Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles |
| title_short | Synthesis, Self-Assembly, and Cell Responses of Aromatic IKVAV Peptide Amphiphiles |
| title_sort | synthesis self assembly and cell responses of aromatic ikvav peptide amphiphiles |
| topic | self-assembly peptide amphiphile hydrogel biomaterial |
| url | https://www.mdpi.com/1420-3049/27/13/4115 |
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