An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis
Abstract Background In Arabidopsis, the aluminum (Al) exclusion mechanism is mainly facilitated by ALMT1-mediated malate exudation and MATE-mediated citrate releases from the root. Recently, we have demonstrated that coordinated functioning between an ALMT1-mediated Al exclusion mechanism, via exuda...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-03-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02338-y |
| _version_ | 1819111158045474816 |
|---|---|
| author | Yuqi Wang Wancong Yu Yu Cao Yanfei Cai Sangbom M. Lyi Weiwei Wu Yan Kang Cuiyue Liang Jiping Liu |
| author_facet | Yuqi Wang Wancong Yu Yu Cao Yanfei Cai Sangbom M. Lyi Weiwei Wu Yan Kang Cuiyue Liang Jiping Liu |
| author_sort | Yuqi Wang |
| collection | DOAJ |
| description | Abstract Background In Arabidopsis, the aluminum (Al) exclusion mechanism is mainly facilitated by ALMT1-mediated malate exudation and MATE-mediated citrate releases from the root. Recently, we have demonstrated that coordinated functioning between an ALMT1-mediated Al exclusion mechanism, via exudation of malate from the root tip, and a NIP1;2-facilitated internal detoxification mechanism, via removal of Al from the root cell wall and subsequent root-to-shoot Al translocation, plays critical roles in achieving overall Al resistance. However, the genetic relationship between ALMT1 and NIP1;2 in these processes remained unclear. Results Through genetic and physiological analyses, we demonstrate that unlike ALMT1 and MATE, which function independently and additively, ALMT1 and NIP1;2 show an epistatic relationship in Al resistance. These results indicate that ALMT1 and NIP1;2 function in the same biochemical pathway, whereas ALMT1 and MATE in different ones. Conclusion The establishment of the epistatic relationship and the coordinated functioning between the ALMT1 and NIP1;2-mediated exclusion and internal detoxification mechanisms are pivotal for achieving overall Al resistance in the non-accumulating Arabidopsis plant. We discuss and emphasize the indispensable roles of the root cell wall for the implementation of the Al exclusion mechanism and for the establishment of an epistatic relationship between the ALMT1-mediated exclusion mechanism and the NIP1;2-facilitated internal detoxification mechanism. |
| first_indexed | 2024-12-22T03:53:10Z |
| format | Article |
| id | doaj.art-789b69c7381b4e3db2caed06b1170688 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-22T03:53:10Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-789b69c7381b4e3db2caed06b11706882022-12-21T18:39:56ZengBMCBMC Plant Biology1471-22292020-03-0120111210.1186/s12870-020-02338-yAn exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in ArabidopsisYuqi Wang0Wancong Yu1Yu Cao2Yanfei Cai3Sangbom M. Lyi4Weiwei Wu5Yan Kang6Cuiyue Liang7Jiping Liu8Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityRobert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell UniversityAbstract Background In Arabidopsis, the aluminum (Al) exclusion mechanism is mainly facilitated by ALMT1-mediated malate exudation and MATE-mediated citrate releases from the root. Recently, we have demonstrated that coordinated functioning between an ALMT1-mediated Al exclusion mechanism, via exudation of malate from the root tip, and a NIP1;2-facilitated internal detoxification mechanism, via removal of Al from the root cell wall and subsequent root-to-shoot Al translocation, plays critical roles in achieving overall Al resistance. However, the genetic relationship between ALMT1 and NIP1;2 in these processes remained unclear. Results Through genetic and physiological analyses, we demonstrate that unlike ALMT1 and MATE, which function independently and additively, ALMT1 and NIP1;2 show an epistatic relationship in Al resistance. These results indicate that ALMT1 and NIP1;2 function in the same biochemical pathway, whereas ALMT1 and MATE in different ones. Conclusion The establishment of the epistatic relationship and the coordinated functioning between the ALMT1 and NIP1;2-mediated exclusion and internal detoxification mechanisms are pivotal for achieving overall Al resistance in the non-accumulating Arabidopsis plant. We discuss and emphasize the indispensable roles of the root cell wall for the implementation of the Al exclusion mechanism and for the establishment of an epistatic relationship between the ALMT1-mediated exclusion mechanism and the NIP1;2-facilitated internal detoxification mechanism.http://link.springer.com/article/10.1186/s12870-020-02338-yALMT1EpistasisMalateNIP1;2Organic acidResistance mechanism |
| spellingShingle | Yuqi Wang Wancong Yu Yu Cao Yanfei Cai Sangbom M. Lyi Weiwei Wu Yan Kang Cuiyue Liang Jiping Liu An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis BMC Plant Biology ALMT1 Epistasis Malate NIP1;2 Organic acid Resistance mechanism |
| title | An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis |
| title_full | An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis |
| title_fullStr | An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis |
| title_full_unstemmed | An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis |
| title_short | An exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in Arabidopsis |
| title_sort | exclusion mechanism is epistatic to an internal detoxification mechanism in aluminum resistance in arabidopsis |
| topic | ALMT1 Epistasis Malate NIP1;2 Organic acid Resistance mechanism |
| url | http://link.springer.com/article/10.1186/s12870-020-02338-y |
| work_keys_str_mv | AT yuqiwang anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT wancongyu anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yucao anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yanfeicai anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT sangbommlyi anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT weiweiwu anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yankang anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT cuiyueliang anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT jipingliu anexclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yuqiwang exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT wancongyu exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yucao exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yanfeicai exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT sangbommlyi exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT weiweiwu exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT yankang exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT cuiyueliang exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis AT jipingliu exclusionmechanismisepistatictoaninternaldetoxificationmechanisminaluminumresistanceinarabidopsis |