Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
Introduction One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-hu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2021-06-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/-Equisetum-arvense-L-aqueous-extract-a-novel-chemotherapeutic-supplement-for-treatment,138146,0,2.html |
| _version_ | 1797435467349622784 |
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| author | Lei Wang Luojun Zhang Guangtao Zheng Haiping Luo Attalla F. El-kott Ayman E. El-kenawy |
| author_facet | Lei Wang Luojun Zhang Guangtao Zheng Haiping Luo Attalla F. El-kott Ayman E. El-kenawy |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Introduction
One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-human lung cancer properties of Equisetum arvense were investigated in in vitro conditions.
Material and methods
Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were assessed to evaluate the antioxidant activity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was chosen to investigate anticancer activity of the plant extract.
Results
The plant extract scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical with an IC50 of 12.3 ±0.7 µg/ml better than positive controls. The plant was also rich in phenolic compounds with an amount of 396.2 ±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of Equisetum arvense L against human colorectal cancer.
Conclusions
The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity towards the normal (HUVEC) cell line. The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines were observed in the case of the HT 29 cell line. |
| first_indexed | 2024-03-09T10:48:29Z |
| format | Article |
| id | doaj.art-78a20c4d26fc45aeaed4865a6608aa33 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:48:29Z |
| publishDate | 2021-06-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-78a20c4d26fc45aeaed4865a6608aa332023-12-01T09:49:08ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512021-06-011951472147810.5114/aoms/138146138146Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinomaLei Wang0Luojun Zhang1Guangtao Zheng2Haiping Luo3Attalla F. El-kott4Ayman E. El-kenawy5Department of Gastrointestinal Surgery, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, ChinaClinical Laboratory, the Second People’s Hospital of Nantong, Nantong, Jiangsu Province, ChinaDepartment of Gastrointestinal Surgery, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Gastrointestinal surgery, Huangshi Central Hospital, affiliated hospital of Hubei polytechnic university, Edong Healthcare Group, Huangshi City, Hubei Province, ChinaBiology Department, College of Science, King Khalid University, Abha, Saudi ArabiaPathology Department, College of Medicine, Taif University, Taif, Saudi ArabiaIntroduction One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-human lung cancer properties of Equisetum arvense were investigated in in vitro conditions. Material and methods Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were assessed to evaluate the antioxidant activity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was chosen to investigate anticancer activity of the plant extract. Results The plant extract scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical with an IC50 of 12.3 ±0.7 µg/ml better than positive controls. The plant was also rich in phenolic compounds with an amount of 396.2 ±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of Equisetum arvense L against human colorectal cancer. Conclusions The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity towards the normal (HUVEC) cell line. The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines were observed in the case of the HT 29 cell line.https://www.archivesofmedicalscience.com/-Equisetum-arvense-L-aqueous-extract-a-novel-chemotherapeutic-supplement-for-treatment,138146,0,2.htmlequisetum arvense lcolorectal carcinomacytotoxicityantioxidant |
| spellingShingle | Lei Wang Luojun Zhang Guangtao Zheng Haiping Luo Attalla F. El-kott Ayman E. El-kenawy Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma Archives of Medical Science equisetum arvense l colorectal carcinoma cytotoxicity antioxidant |
| title | Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma |
| title_full | Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma |
| title_fullStr | Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma |
| title_full_unstemmed | Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma |
| title_short | Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma |
| title_sort | equisetum arvense l aqueous extract a novel chemotherapeutic supplement for treatment of human colon carcinoma |
| topic | equisetum arvense l colorectal carcinoma cytotoxicity antioxidant |
| url | https://www.archivesofmedicalscience.com/-Equisetum-arvense-L-aqueous-extract-a-novel-chemotherapeutic-supplement-for-treatment,138146,0,2.html |
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