An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle
Summary: Adherent-invasive Escherichia coli (AIEC) is an opportunistic pathogen associated with major inflammatory bowel disease, Crohn disease, and ulcerative colitis. Unfavorable conditions push commensal AIEC to induce gut inflammation, sometimes progressing to inflammation-induced colon cancer....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222008446 |
| _version_ | 1811240940088590336 |
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| author | Dhrubajyoti Nag Dustin Farr Saumya Raychaudhuri Jeffrey H. Withey |
| author_facet | Dhrubajyoti Nag Dustin Farr Saumya Raychaudhuri Jeffrey H. Withey |
| author_sort | Dhrubajyoti Nag |
| collection | DOAJ |
| description | Summary: Adherent-invasive Escherichia coli (AIEC) is an opportunistic pathogen associated with major inflammatory bowel disease, Crohn disease, and ulcerative colitis. Unfavorable conditions push commensal AIEC to induce gut inflammation, sometimes progressing to inflammation-induced colon cancer. Recently, zebrafish have emerged as a useful model to study human intestinal pathogens. Here, a zebrafish model to study AIEC infection was developed. Bath inoculation with AIEC resulted in colonization and tissue disruption in the zebrafish intestine. Gene expression of pro-inflammatory markers including interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFα), interferon-γ (IFNγ), and S100A-10b (akin to human calprotectin) in the zebrafish intestine was significantly induced by AIEC infection. The probiotic E. coli Nissle 1917 (EcN) was tested as a therapeutic and prophylactic against AIEC infection and reduced AIEC colonization, tissue damage, and pro-inflammatory responses in zebrafish. Furthermore, EcN diminished the propionic-acid-augmented hyperinfection of AIEC in zebrafish. Thus, this study shows the efficacy of EcN against AIEC in an AIEC-zebrafish model. |
| first_indexed | 2024-04-12T13:29:26Z |
| format | Article |
| id | doaj.art-78a439697c6d43deb80b67470613c31a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T13:29:26Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-78a439697c6d43deb80b67470613c31a2022-12-22T03:31:14ZengElsevieriScience2589-00422022-07-01257104572An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli NissleDhrubajyoti Nag0Dustin Farr1Saumya Raychaudhuri2Jeffrey H. Withey3Department of Biochemistry, Microbiology, and Immunology, Wayne State University School of Medicine, Detroit, MI, USADepartment of Biochemistry, Microbiology, and Immunology, Wayne State University School of Medicine, Detroit, MI, USACSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, IndiaDepartment of Biochemistry, Microbiology, and Immunology, Wayne State University School of Medicine, Detroit, MI, USA; Corresponding authorSummary: Adherent-invasive Escherichia coli (AIEC) is an opportunistic pathogen associated with major inflammatory bowel disease, Crohn disease, and ulcerative colitis. Unfavorable conditions push commensal AIEC to induce gut inflammation, sometimes progressing to inflammation-induced colon cancer. Recently, zebrafish have emerged as a useful model to study human intestinal pathogens. Here, a zebrafish model to study AIEC infection was developed. Bath inoculation with AIEC resulted in colonization and tissue disruption in the zebrafish intestine. Gene expression of pro-inflammatory markers including interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFα), interferon-γ (IFNγ), and S100A-10b (akin to human calprotectin) in the zebrafish intestine was significantly induced by AIEC infection. The probiotic E. coli Nissle 1917 (EcN) was tested as a therapeutic and prophylactic against AIEC infection and reduced AIEC colonization, tissue damage, and pro-inflammatory responses in zebrafish. Furthermore, EcN diminished the propionic-acid-augmented hyperinfection of AIEC in zebrafish. Thus, this study shows the efficacy of EcN against AIEC in an AIEC-zebrafish model.http://www.sciencedirect.com/science/article/pii/S2589004222008446PathophysiologyMicrobiology |
| spellingShingle | Dhrubajyoti Nag Dustin Farr Saumya Raychaudhuri Jeffrey H. Withey An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle iScience Pathophysiology Microbiology |
| title | An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle |
| title_full | An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle |
| title_fullStr | An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle |
| title_full_unstemmed | An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle |
| title_short | An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle |
| title_sort | adult zebrafish model for adherent invasive escherichia coli indicates protection from aiec infection by probiotic e coli nissle |
| topic | Pathophysiology Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222008446 |
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