<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
<i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective eff...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/11/2680 |
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| author | Mengqi Li Qi Ge Hanting Du Songyi Lin |
| author_facet | Mengqi Li Qi Ge Hanting Du Songyi Lin |
| author_sort | Mengqi Li |
| collection | DOAJ |
| description | <i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective effects and molecular mechanisms of two <i>T. matsutake</i>-derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. <i>Tricholoma matsutake</i> peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of <i>T. matsutake</i> peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with <i>T. matsutake</i> peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that <i>T. matsutake</i> peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage. |
| first_indexed | 2024-03-10T05:30:33Z |
| format | Article |
| id | doaj.art-78a8c860a1364876be7b9dba0dac7843 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:30:33Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-78a8c860a1364876be7b9dba0dac78432023-11-22T23:20:28ZengMDPI AGFoods2304-81582021-11-011011268010.3390/foods10112680<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 PathwayMengqi Li0Qi Ge1Hanting Du2Songyi Lin3National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China<i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective effects and molecular mechanisms of two <i>T. matsutake</i>-derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. <i>Tricholoma matsutake</i> peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of <i>T. matsutake</i> peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with <i>T. matsutake</i> peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that <i>T. matsutake</i> peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage.https://www.mdpi.com/2304-8158/10/11/2680<i>Tricholoma matsutake</i>RAW264.7 macrophagesinflammationNF-κBmitochondrial dysfunction |
| spellingShingle | Mengqi Li Qi Ge Hanting Du Songyi Lin <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway Foods <i>Tricholoma matsutake</i> RAW264.7 macrophages inflammation NF-κB mitochondrial dysfunction |
| title | <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway |
| title_full | <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway |
| title_fullStr | <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway |
| title_full_unstemmed | <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway |
| title_short | <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway |
| title_sort | i tricholoma matsutake i derived peptides ameliorate inflammation and mitochondrial dysfunction in raw264 7 macrophages by modulating the nf κb cox 2 pathway |
| topic | <i>Tricholoma matsutake</i> RAW264.7 macrophages inflammation NF-κB mitochondrial dysfunction |
| url | https://www.mdpi.com/2304-8158/10/11/2680 |
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