A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment
A thorough understanding of microbial communities in the gut of lower termites is needed to develop target-specific and environmentally benign wood protection systems. In this study, the bacterial community from <i>Reticulitermes virginicus</i> was examined by Illumina sequencing of 16S...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/10/681 |
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| author | Telmah Telmadarrehei Juliet D. Tang Olanrewaju Raji Amir Rezazadeh Lakshmi Narayanan Rubin Shmulsky Dragica Jeremic |
| author_facet | Telmah Telmadarrehei Juliet D. Tang Olanrewaju Raji Amir Rezazadeh Lakshmi Narayanan Rubin Shmulsky Dragica Jeremic |
| author_sort | Telmah Telmadarrehei |
| collection | DOAJ |
| description | A thorough understanding of microbial communities in the gut of lower termites is needed to develop target-specific and environmentally benign wood protection systems. In this study, the bacterial community from <i>Reticulitermes virginicus</i> was examined by Illumina sequencing of 16S ribosomal RNA (rRNA) spanning the V3 and V4 regions. Prior to library preparation, the termites were subjected to five treatments over an 18-day period: three groups were fed on wood treated with 0.5% chitosan, 25% acetic acid, or water, the fourth group was taken directly from the original collection log, and the fifth group was starved. Metagenomic sequences were analyzed using QIIME 2 to understand the treatments’ effects on the dynamics of the gut bacteria. Four dominant phyla were detected: Bacteroidetes (34.4% of reads), Firmicutes (20.6%), Elusimicrobia (15.7%), and Proteobacteria (12.9%). A significant effect of chitosan treatment was observed in two phyla; Firmicutes abundance was significantly lower with chitosan treatment when compared to other groups, while Actinobacteria was lower in unexposed and starved termites. The results suggest that chitosan treatment not only affects the structure of the microbial community in the gut, but other treatments such as starving also cause shifts in termite gut communities. |
| first_indexed | 2024-03-10T15:47:53Z |
| format | Article |
| id | doaj.art-78a904586c8246bf9dfb81a4e41dc40d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T15:47:53Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-78a904586c8246bf9dfb81a4e41dc40d2023-11-20T16:19:40ZengMDPI AGInsects2075-44502020-10-01111068110.3390/insects11100681A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan TreatmentTelmah Telmadarrehei0Juliet D. Tang1Olanrewaju Raji2Amir Rezazadeh3Lakshmi Narayanan4Rubin Shmulsky5Dragica Jeremic6Department of Sustainable Bioproducts, Mississippi State University, 201 Locksley Way, Starkville, MS 39759, USAForest Products Laboratory, USDA Forest Service, 201 Lincoln Green, Starkville, MS 39759, USADepartment of Sustainable Bioproducts, Mississippi State University, 201 Locksley Way, Starkville, MS 39759, USAInstitute of Food and Agriculture Science, University of Florida, 8400 Picos Road, Ste. 101, Fort Pierce, FL 34945, USADepartment of Sustainable Bioproducts, Mississippi State University, 201 Locksley Way, Starkville, MS 39759, USADepartment of Sustainable Bioproducts, Mississippi State University, 201 Locksley Way, Starkville, MS 39759, USADepartment of Sustainable Bioproducts, Mississippi State University, 201 Locksley Way, Starkville, MS 39759, USAA thorough understanding of microbial communities in the gut of lower termites is needed to develop target-specific and environmentally benign wood protection systems. In this study, the bacterial community from <i>Reticulitermes virginicus</i> was examined by Illumina sequencing of 16S ribosomal RNA (rRNA) spanning the V3 and V4 regions. Prior to library preparation, the termites were subjected to five treatments over an 18-day period: three groups were fed on wood treated with 0.5% chitosan, 25% acetic acid, or water, the fourth group was taken directly from the original collection log, and the fifth group was starved. Metagenomic sequences were analyzed using QIIME 2 to understand the treatments’ effects on the dynamics of the gut bacteria. Four dominant phyla were detected: Bacteroidetes (34.4% of reads), Firmicutes (20.6%), Elusimicrobia (15.7%), and Proteobacteria (12.9%). A significant effect of chitosan treatment was observed in two phyla; Firmicutes abundance was significantly lower with chitosan treatment when compared to other groups, while Actinobacteria was lower in unexposed and starved termites. The results suggest that chitosan treatment not only affects the structure of the microbial community in the gut, but other treatments such as starving also cause shifts in termite gut communities.https://www.mdpi.com/2075-4450/11/10/681chitosan<i>Reticulitermes virginicus</i>gut bacteria16S rRNAIllumina amplicon sequencingmetagenomics |
| spellingShingle | Telmah Telmadarrehei Juliet D. Tang Olanrewaju Raji Amir Rezazadeh Lakshmi Narayanan Rubin Shmulsky Dragica Jeremic A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment Insects chitosan <i>Reticulitermes virginicus</i> gut bacteria 16S rRNA Illumina amplicon sequencing metagenomics |
| title | A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment |
| title_full | A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment |
| title_fullStr | A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment |
| title_full_unstemmed | A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment |
| title_short | A Study of the Gut Bacterial Community of <i>Reticulitermes virginicus</i> Exposed to Chitosan Treatment |
| title_sort | study of the gut bacterial community of i reticulitermes virginicus i exposed to chitosan treatment |
| topic | chitosan <i>Reticulitermes virginicus</i> gut bacteria 16S rRNA Illumina amplicon sequencing metagenomics |
| url | https://www.mdpi.com/2075-4450/11/10/681 |
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