Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium
The basidiomycetous yeast, <i>Glaciozyma antarctica</i>, was isolated from various terrestrial materials collected from the Sôya coast, East Antarctica, and formed frost-columnar colonies on agar plates frozen at −1 °C. Thawed colonies were highly viscous, indicating that the yeast produ...
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MDPI AG
2021-08-01
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| author | Seiichi Fujiu Masanobu Ito Eriko Kobayashi Yuichi Hanada Midori Yoshida Sakae Kudoh Tamotsu Hoshino |
| author_facet | Seiichi Fujiu Masanobu Ito Eriko Kobayashi Yuichi Hanada Midori Yoshida Sakae Kudoh Tamotsu Hoshino |
| author_sort | Seiichi Fujiu |
| collection | DOAJ |
| description | The basidiomycetous yeast, <i>Glaciozyma antarctica</i>, was isolated from various terrestrial materials collected from the Sôya coast, East Antarctica, and formed frost-columnar colonies on agar plates frozen at −1 °C. Thawed colonies were highly viscous, indicating that the yeast produced a large number of extracellular polysaccharides (EPS). <i>G. antarctica</i> was then cultured on frozen media containing red food coloring to observe the dynamics of solutes in unfrozen water; pigments accumulated in frozen yeast colonies, indicating that solutes were concentrated in unfrozen water of yeast colonies. Moreover, the yeast produced a small quantity of ice-binding proteins (IBPs) which inhibited ice crystal growth. Solutes in unfrozen water were considered to accumulate in the pore of frozen colonies. The extracellular IBPs may have held an unfrozen state of medium water after accumulation in the frost-columnar colony. |
| first_indexed | 2024-03-10T08:34:15Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:15Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-78a984cce6da482780a4af09595a15012023-11-22T08:47:17ZengMDPI AGMicroorganisms2076-26072021-08-0198167910.3390/microorganisms9081679Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen MediumSeiichi Fujiu0Masanobu Ito1Eriko Kobayashi2Yuichi Hanada3Midori Yoshida4Sakae Kudoh5Tamotsu Hoshino6Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-higashi, Toyohira-ku, Sapporo 062-8517, Hokkaido, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-higashi, Toyohira-ku, Sapporo 062-8517, Hokkaido, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-higashi, Toyohira-ku, Sapporo 062-8517, Hokkaido, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-higashi, Toyohira-ku, Sapporo 062-8517, Hokkaido, JapanHokkaido Agricultural Research Center, NARO, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Hokkaido, JapanBiology Group, National Institute of Polar Research, 10-3, Midori-cho, Tachikawa, Tokyo 190-8518, JapanBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-higashi, Toyohira-ku, Sapporo 062-8517, Hokkaido, JapanThe basidiomycetous yeast, <i>Glaciozyma antarctica</i>, was isolated from various terrestrial materials collected from the Sôya coast, East Antarctica, and formed frost-columnar colonies on agar plates frozen at −1 °C. Thawed colonies were highly viscous, indicating that the yeast produced a large number of extracellular polysaccharides (EPS). <i>G. antarctica</i> was then cultured on frozen media containing red food coloring to observe the dynamics of solutes in unfrozen water; pigments accumulated in frozen yeast colonies, indicating that solutes were concentrated in unfrozen water of yeast colonies. Moreover, the yeast produced a small quantity of ice-binding proteins (IBPs) which inhibited ice crystal growth. Solutes in unfrozen water were considered to accumulate in the pore of frozen colonies. The extracellular IBPs may have held an unfrozen state of medium water after accumulation in the frost-columnar colony.https://www.mdpi.com/2076-2607/9/8/1679cold adaptationecological strategyfrost resistanceice-binding proteins (IBPs)polysaccharideunfrozen water |
| spellingShingle | Seiichi Fujiu Masanobu Ito Eriko Kobayashi Yuichi Hanada Midori Yoshida Sakae Kudoh Tamotsu Hoshino Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium Microorganisms cold adaptation ecological strategy frost resistance ice-binding proteins (IBPs) polysaccharide unfrozen water |
| title | Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium |
| title_full | Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium |
| title_fullStr | Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium |
| title_full_unstemmed | Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium |
| title_short | Basidiomycetous Yeast, <i>Glaciozyma antarctica,</i> Forming Frost-Columnar Colonies on Frozen Medium |
| title_sort | basidiomycetous yeast i glaciozyma antarctica i forming frost columnar colonies on frozen medium |
| topic | cold adaptation ecological strategy frost resistance ice-binding proteins (IBPs) polysaccharide unfrozen water |
| url | https://www.mdpi.com/2076-2607/9/8/1679 |
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