Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate
The present systematic research on cultural, morphological, and pathogenic variability was carried out on eighty isolates of <i>Sclerotinia sclerotiorum</i> collected from major common bean production belts of North Kashmir. The isolates were found to vary in both cultural and morphologi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/7/755 |
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| author | Roaf Ahmad Rather Farooq Ahmad Ahanger Shafat Ahmad Ahanger Umer Basu M. Altaf Wani Zahida Rashid Parvaze Ahmad Sofi Vishal Singh Kounser Javeed Alaa Baazeem Saqer S. Alotaibi Owais Ali Wani Jasima Ali Khanday Showket Ahmad Dar Muntazir Mushtaq |
| author_facet | Roaf Ahmad Rather Farooq Ahmad Ahanger Shafat Ahmad Ahanger Umer Basu M. Altaf Wani Zahida Rashid Parvaze Ahmad Sofi Vishal Singh Kounser Javeed Alaa Baazeem Saqer S. Alotaibi Owais Ali Wani Jasima Ali Khanday Showket Ahmad Dar Muntazir Mushtaq |
| author_sort | Roaf Ahmad Rather |
| collection | DOAJ |
| description | The present systematic research on cultural, morphological, and pathogenic variability was carried out on eighty isolates of <i>Sclerotinia sclerotiorum</i> collected from major common bean production belts of North Kashmir. The isolates were found to vary in both cultural and morphological characteristics such as colony color and type, colony diameter, number of days for sclerotia initiation, sclerotia number per plate, sclerotial weight, and size. The colony color ranged between white and off-white with the majority. The colony was of three types, in majority smooth, some fluffy, and a few fluffy-at-center-only. Colony diameter ranged between 15.33 mm and 29 mm after 24 h of incubation. The isolates took 4 to 7 days for initiation of sclerotia and varied in size, weight, and number per plate ranging between 14 and 51.3. The sclerotial arrangement pattern on plates was peripheral, sub peripheral, peripheral, and subperipheral, arranged at the rim and scattered. A total of 22 Mycelial compatibility groups (MCGs) were formed with seven groups constituted by a single isolate. The isolates within MCGs were mostly at par with each other. The six isolates representing six MCGs showed variability in pathogenicity with isolate G04 as the most and B01 as the least virulent. The colony diameter and disease scores were positively correlated. Sclerotia were observed to germinate both myceliogenically and carpogenically under natural temperate conditions of Kashmir. Germplasm screening revealed a single resistant line and eleven partially resistant lines against most virulent isolates. |
| first_indexed | 2024-03-09T03:18:00Z |
| format | Article |
| id | doaj.art-78a9ab86708d450ba2330ebf15da4cd8 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T03:18:00Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-78a9ab86708d450ba2330ebf15da4cd82023-12-03T15:16:04ZengMDPI AGJournal of Fungi2309-608X2022-07-018775510.3390/jof8070755Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate ClimateRoaf Ahmad Rather0Farooq Ahmad Ahanger1Shafat Ahmad Ahanger2Umer Basu3M. Altaf Wani4Zahida Rashid5Parvaze Ahmad Sofi6Vishal Singh7Kounser Javeed8Alaa Baazeem9Saqer S. Alotaibi10Owais Ali Wani11Jasima Ali Khanday12Showket Ahmad Dar13Muntazir Mushtaq14Division of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaKrishi Vigyan Kendra, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama, Ganderbal 190006, IndiaDivision of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaDivision of Entomology, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics and Plant Breeding, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaDry Land Agriculture Research Station, Rangreth, SKUAST-K, Shalimar 190025, IndiaDivision of Genetics and Plant Breeding, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaGenetics and Plant Breeding, Dr. Rammanohar Lohia Avadh University, Ayodhya 224123, IndiaDivision of Fruit Science, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDivision of Soil Science and Agricultural Chemistry, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaDivision of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, Sopore 193201, IndiaFaculty of Forestry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Benhama, Ganderbal 191202, IndiaSchool of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu 180009, IndiaThe present systematic research on cultural, morphological, and pathogenic variability was carried out on eighty isolates of <i>Sclerotinia sclerotiorum</i> collected from major common bean production belts of North Kashmir. The isolates were found to vary in both cultural and morphological characteristics such as colony color and type, colony diameter, number of days for sclerotia initiation, sclerotia number per plate, sclerotial weight, and size. The colony color ranged between white and off-white with the majority. The colony was of three types, in majority smooth, some fluffy, and a few fluffy-at-center-only. Colony diameter ranged between 15.33 mm and 29 mm after 24 h of incubation. The isolates took 4 to 7 days for initiation of sclerotia and varied in size, weight, and number per plate ranging between 14 and 51.3. The sclerotial arrangement pattern on plates was peripheral, sub peripheral, peripheral, and subperipheral, arranged at the rim and scattered. A total of 22 Mycelial compatibility groups (MCGs) were formed with seven groups constituted by a single isolate. The isolates within MCGs were mostly at par with each other. The six isolates representing six MCGs showed variability in pathogenicity with isolate G04 as the most and B01 as the least virulent. The colony diameter and disease scores were positively correlated. Sclerotia were observed to germinate both myceliogenically and carpogenically under natural temperate conditions of Kashmir. Germplasm screening revealed a single resistant line and eleven partially resistant lines against most virulent isolates.https://www.mdpi.com/2309-608X/8/7/755common beangermplasmmycelial compatibility groupspathogenic variability<i>Sclerotinia sclerotiorum</i>white mold |
| spellingShingle | Roaf Ahmad Rather Farooq Ahmad Ahanger Shafat Ahmad Ahanger Umer Basu M. Altaf Wani Zahida Rashid Parvaze Ahmad Sofi Vishal Singh Kounser Javeed Alaa Baazeem Saqer S. Alotaibi Owais Ali Wani Jasima Ali Khanday Showket Ahmad Dar Muntazir Mushtaq Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate Journal of Fungi common bean germplasm mycelial compatibility groups pathogenic variability <i>Sclerotinia sclerotiorum</i> white mold |
| title | Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate |
| title_full | Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate |
| title_fullStr | Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate |
| title_full_unstemmed | Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate |
| title_short | Morpho-Cultural and Pathogenic Variability of <i>Sclerotinia sclerotiorum</i> Causing White Mold of Common Beans in Temperate Climate |
| title_sort | morpho cultural and pathogenic variability of i sclerotinia sclerotiorum i causing white mold of common beans in temperate climate |
| topic | common bean germplasm mycelial compatibility groups pathogenic variability <i>Sclerotinia sclerotiorum</i> white mold |
| url | https://www.mdpi.com/2309-608X/8/7/755 |
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