MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis
Cell size control is one of the prerequisites for plant growth and development. Recently, a GRAS family transcription factor, SCARECROW-LIKE28 (SCL28), was identified as a critical regulator for both mitotic and postmitotic cell-size control. Here, we show that SCL28 is specifically expressed in pro...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2022.2153209 |
| _version_ | 1797351898619052032 |
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| author | Hirotomo Takatsuka Yuji Nomoto Kesuke Yamada Keito Mineta Christian Breuer Takashi Ishida Ayumi Yamagami Keiko Sugimoto Takeshi Nakano Masaki Ito |
| author_facet | Hirotomo Takatsuka Yuji Nomoto Kesuke Yamada Keito Mineta Christian Breuer Takashi Ishida Ayumi Yamagami Keiko Sugimoto Takeshi Nakano Masaki Ito |
| author_sort | Hirotomo Takatsuka |
| collection | DOAJ |
| description | Cell size control is one of the prerequisites for plant growth and development. Recently, a GRAS family transcription factor, SCARECROW-LIKE28 (SCL28), was identified as a critical regulator for both mitotic and postmitotic cell-size control. Here, we show that SCL28 is specifically expressed in proliferating cells and exerts its function to delay G2 progression during mitotic cell cycle in Arabidopsis thaliana. Overexpression of SCL28 provokes a significant enlargement of cells in various organs and tissues, such as leaves, flowers and seeds, to different extents depending on the type of cells. The increased cell size is most likely due to a delayed G2 progression and accelerated onset of endoreplication, an atypical cell cycle repeating DNA replication without cytokinesis or mitosis. Unlike DWARF AND LOW-TILLERING, a rice ortholog of SCL28, SCL28 may not have a role in brassinosteroid (BR) signaling because sensitivity against brassinazole, a BR biosynthesis inhibitor, was not dramatically altered in scl28 mutant and SCL28-overexpressing plants. Collectively, our findings strengthen a recently proposed model of cell size control by SCL28 and suggest the presence of diversified evolutionary mechanisms for the regulation and action of SCL28. |
| first_indexed | 2024-03-08T13:08:23Z |
| format | Article |
| id | doaj.art-78aeb33eeca940ef83ab9c89e6f5e660 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:23Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-78aeb33eeca940ef83ab9c89e6f5e6602024-01-18T15:58:22ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242023-12-0118110.1080/15592324.2022.21532092153209MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in ArabidopsisHirotomo Takatsuka0Yuji Nomoto1Kesuke Yamada2Keito Mineta3Christian Breuer4Takashi Ishida5Ayumi Yamagami6Keiko Sugimoto7Takeshi Nakano8Masaki Ito9School of Biological Science and Technology, College of Science and Engineering, Kanazawa UniversitySchool of Biological Science and Technology, College of Science and Engineering, Kanazawa UniversitySchool of Biological Science and Technology, College of Science and Engineering, Kanazawa UniversitySchool of Biological Science and Technology, College of Science and Engineering, Kanazawa UniversityRIKEN Center for Sustainable Resource ScienceRIKEN Center for Sustainable Resource ScienceGraduate School of Biostudies, Kyoto UniversityRIKEN Center for Sustainable Resource ScienceGraduate School of Biostudies, Kyoto UniversitySchool of Biological Science and Technology, College of Science and Engineering, Kanazawa UniversityCell size control is one of the prerequisites for plant growth and development. Recently, a GRAS family transcription factor, SCARECROW-LIKE28 (SCL28), was identified as a critical regulator for both mitotic and postmitotic cell-size control. Here, we show that SCL28 is specifically expressed in proliferating cells and exerts its function to delay G2 progression during mitotic cell cycle in Arabidopsis thaliana. Overexpression of SCL28 provokes a significant enlargement of cells in various organs and tissues, such as leaves, flowers and seeds, to different extents depending on the type of cells. The increased cell size is most likely due to a delayed G2 progression and accelerated onset of endoreplication, an atypical cell cycle repeating DNA replication without cytokinesis or mitosis. Unlike DWARF AND LOW-TILLERING, a rice ortholog of SCL28, SCL28 may not have a role in brassinosteroid (BR) signaling because sensitivity against brassinazole, a BR biosynthesis inhibitor, was not dramatically altered in scl28 mutant and SCL28-overexpressing plants. Collectively, our findings strengthen a recently proposed model of cell size control by SCL28 and suggest the presence of diversified evolutionary mechanisms for the regulation and action of SCL28.http://dx.doi.org/10.1080/15592324.2022.2153209cell cycleg2 phasecell sizetranscription factorgras familymyb3rs |
| spellingShingle | Hirotomo Takatsuka Yuji Nomoto Kesuke Yamada Keito Mineta Christian Breuer Takashi Ishida Ayumi Yamagami Keiko Sugimoto Takeshi Nakano Masaki Ito MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis Plant Signaling & Behavior cell cycle g2 phase cell size transcription factor gras family myb3rs |
| title | MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis |
| title_full | MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis |
| title_fullStr | MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis |
| title_full_unstemmed | MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis |
| title_short | MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in Arabidopsis |
| title_sort | myb3r scl28 smr module with a role in cell size control negatively regulates g2 progression in arabidopsis |
| topic | cell cycle g2 phase cell size transcription factor gras family myb3rs |
| url | http://dx.doi.org/10.1080/15592324.2022.2153209 |
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