Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes
High-temperature ethanol fermentation (HTEF) using high-potential thermotolerant ethanologenic microorganisms is a promising platform for ethanol production in tropical or subtropical areas. This study aims to evaluate the ethanol production potential of recombinant <i>Zymomonas mobilis</i&...
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MDPI AG
2023-07-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/16/14/5284 |
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| author | Kankanok Charoenpunthuwong Preekamol Klanrit Nuttaporn Chamnipa Sudarat Thanonkeo Mamoru Yamada Pornthap Thanonkeo |
| author_facet | Kankanok Charoenpunthuwong Preekamol Klanrit Nuttaporn Chamnipa Sudarat Thanonkeo Mamoru Yamada Pornthap Thanonkeo |
| author_sort | Kankanok Charoenpunthuwong |
| collection | DOAJ |
| description | High-temperature ethanol fermentation (HTEF) using high-potential thermotolerant ethanologenic microorganisms is a promising platform for ethanol production in tropical or subtropical areas. This study aims to evaluate the ethanol production potential of recombinant <i>Zymomonas mobilis</i> R301 overexpressing <i>groESL</i> genes under normal and high-temperature conditions and the expression of genes involved in the heat shock response and ethanol production pathway during ethanol fermentation using sweet sorghum juice (SSJ) as feedstock. Growth characterization analysis revealed that the recombinant <i>Z. mobilis</i> R301 exhibited multi-stress tolerance toward heat, acetic acid, and furfural. Based on the statistical experimental design, the optimum conditions for ethanol production from SSJ by the recombinant R301 at 30 °C were a sugar concentration of 171.67 g/L, cell concentration of 9.42% (<i>v</i>/<i>v</i>), and yeast extract concentration of 10.89 g/L, while those at 40 °C were a sugar concentration of 199.48 g/L, yeast extract concentration of 10.88 g/L, MgSO<sub>4</sub> concentration of 1.05 g/L, and initial pH of 6.8. The maximum ethanol concentrations and productivities achieved in this study were 63.26 g/L and 1.17 g/L.h at 30 °C and 58.62 g/L and 1.22 g/L.h at 40 °C. The overexpression of the <i>groES</i> and <i>groEL</i> genes and upregulation of other heat shock-responsive genes at 40 °C enhanced cell growth, viability, and fermentation capacity of recombinant <i>Z. mobilis</i> R301 under heat stress. The current study demonstrated that recombinant <i>Z. mobilis</i> R301 exhibited high potential for ethanol production from SSJ or other sugar-based raw materials under high-temperature conditions. |
| first_indexed | 2024-03-11T01:07:49Z |
| format | Article |
| id | doaj.art-78af0e5e3a424c6b993a66401cc02054 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-11T01:07:49Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-78af0e5e3a424c6b993a66401cc020542023-11-18T19:08:07ZengMDPI AGEnergies1996-10732023-07-011614528410.3390/en16145284Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> GenesKankanok Charoenpunthuwong0Preekamol Klanrit1Nuttaporn Chamnipa2Sudarat Thanonkeo3Mamoru Yamada4Pornthap Thanonkeo5Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandWalai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, JapanDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandHigh-temperature ethanol fermentation (HTEF) using high-potential thermotolerant ethanologenic microorganisms is a promising platform for ethanol production in tropical or subtropical areas. This study aims to evaluate the ethanol production potential of recombinant <i>Zymomonas mobilis</i> R301 overexpressing <i>groESL</i> genes under normal and high-temperature conditions and the expression of genes involved in the heat shock response and ethanol production pathway during ethanol fermentation using sweet sorghum juice (SSJ) as feedstock. Growth characterization analysis revealed that the recombinant <i>Z. mobilis</i> R301 exhibited multi-stress tolerance toward heat, acetic acid, and furfural. Based on the statistical experimental design, the optimum conditions for ethanol production from SSJ by the recombinant R301 at 30 °C were a sugar concentration of 171.67 g/L, cell concentration of 9.42% (<i>v</i>/<i>v</i>), and yeast extract concentration of 10.89 g/L, while those at 40 °C were a sugar concentration of 199.48 g/L, yeast extract concentration of 10.88 g/L, MgSO<sub>4</sub> concentration of 1.05 g/L, and initial pH of 6.8. The maximum ethanol concentrations and productivities achieved in this study were 63.26 g/L and 1.17 g/L.h at 30 °C and 58.62 g/L and 1.22 g/L.h at 40 °C. The overexpression of the <i>groES</i> and <i>groEL</i> genes and upregulation of other heat shock-responsive genes at 40 °C enhanced cell growth, viability, and fermentation capacity of recombinant <i>Z. mobilis</i> R301 under heat stress. The current study demonstrated that recombinant <i>Z. mobilis</i> R301 exhibited high potential for ethanol production from SSJ or other sugar-based raw materials under high-temperature conditions.https://www.mdpi.com/1996-1073/16/14/5284ethanol<i>groESL</i> geneshigh-temperature fermentationsweet sorghum<i>Zymomonas mobilis</i> |
| spellingShingle | Kankanok Charoenpunthuwong Preekamol Klanrit Nuttaporn Chamnipa Sudarat Thanonkeo Mamoru Yamada Pornthap Thanonkeo Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes Energies ethanol <i>groESL</i> genes high-temperature fermentation sweet sorghum <i>Zymomonas mobilis</i> |
| title | Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes |
| title_full | Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes |
| title_fullStr | Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes |
| title_full_unstemmed | Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes |
| title_short | Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant <i>Zymomonas mobilis</i> Overexpressing <i>groESL</i> Genes |
| title_sort | optimization condition for ethanol production from sweet sorghum juice by recombinant i zymomonas mobilis i overexpressing i groesl i genes |
| topic | ethanol <i>groESL</i> genes high-temperature fermentation sweet sorghum <i>Zymomonas mobilis</i> |
| url | https://www.mdpi.com/1996-1073/16/14/5284 |
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