Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine
Objective(s): To assess the protective effect of L-carnitine in reducing cisplatin toxicity via estimating biochemical tests, histomorphometric, and immunohistochemistry (IHC) of β-catenin and cyclin D.Materials and Methods: Fifteen adult male rabbits were used in this study and allocated into 3 gro...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_20562_e3ca7e8eac303d0c63dc23a0fac4414d.pdf |
| _version_ | 1811192531693600768 |
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| author | snur Hassan Azad K Saeed Omed Omer Rahim Shler A F Mahmood |
| author_facet | snur Hassan Azad K Saeed Omed Omer Rahim Shler A F Mahmood |
| author_sort | snur Hassan |
| collection | DOAJ |
| description | Objective(s): To assess the protective effect of L-carnitine in reducing cisplatin toxicity via estimating biochemical tests, histomorphometric, and immunohistochemistry (IHC) of β-catenin and cyclin D.Materials and Methods: Fifteen adult male rabbits were used in this study and allocated into 3 groups; Group 1 (Control negative), rabbits of this group were not given any treatment. In group 2, the animals were injected with cisplatin single-dose/per week. Group 3 rabbits were treated with Cisplatin+L-carnitine orally by gavage tube for 29 days. At the end of the experiments, blood samples from all rabbits were taken from the earlobe, and then the biochemical test was done, the kidney and tissue sections were prepared for both H& E and IHC for both β-catenin and cyclin D genes.Results: Treatment with L-carnitine reduced the injury effect of cisplatin via a decline in serum creatinine, urea, bilirubin, GPT, GOP, and ALP significantly (P<0.05). Also, administration of LC attenuates the histopathologic abnormality in the kidney (15.71% vs 85.18%) and liver (score 3 vs 15 ) induced by cisplatin. L-carnitine elevates the expression of β-catenin and cyclin D in renal and hepatic parenchyma by diffuse, moderate-strong positivity vs cisplatin that showed local-weak staining.Conclusion: These findings imply that L-carnitine, by its pleiotropic actions in activating Wnt signaling, alleviates cisplatin-induced renal and hepatic destruction. It might be a method of preventing cisplatin-related nephrotoxicity and hepatotoxicity. |
| first_indexed | 2024-04-11T23:54:10Z |
| format | Article |
| id | doaj.art-78af1af29af349379eb21ca1616ff9d3 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T23:54:10Z |
| publishDate | 2022-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-78af1af29af349379eb21ca1616ff9d32022-12-22T03:56:24ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-07-0125789790310.22038/ijbms.2022.65427.1439520562Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitinesnur Hassan0Azad K Saeed1Omed Omer Rahim2Shler A F Mahmood3Department of Anatomy and Histopathology, College of Veterinary Medicine, Sulaimani University, Sulaimani, 4601, KRG, IraqDepartment of Anatomy and Histopathology, College of Veterinary Medicine, Sulaimani University, Sulaimani, 4601, KRG, IraqDepartment of Food Science and Quality Control, Bakrajo Technical Institute, Sulaimani Polytechnic University, Sulaimani, 4601, KRG, IraqDepartment of Microbiology, School of Medicine, Sulaimani University, Sulaimani, 4601, KRG, IraqObjective(s): To assess the protective effect of L-carnitine in reducing cisplatin toxicity via estimating biochemical tests, histomorphometric, and immunohistochemistry (IHC) of β-catenin and cyclin D.Materials and Methods: Fifteen adult male rabbits were used in this study and allocated into 3 groups; Group 1 (Control negative), rabbits of this group were not given any treatment. In group 2, the animals were injected with cisplatin single-dose/per week. Group 3 rabbits were treated with Cisplatin+L-carnitine orally by gavage tube for 29 days. At the end of the experiments, blood samples from all rabbits were taken from the earlobe, and then the biochemical test was done, the kidney and tissue sections were prepared for both H& E and IHC for both β-catenin and cyclin D genes.Results: Treatment with L-carnitine reduced the injury effect of cisplatin via a decline in serum creatinine, urea, bilirubin, GPT, GOP, and ALP significantly (P<0.05). Also, administration of LC attenuates the histopathologic abnormality in the kidney (15.71% vs 85.18%) and liver (score 3 vs 15 ) induced by cisplatin. L-carnitine elevates the expression of β-catenin and cyclin D in renal and hepatic parenchyma by diffuse, moderate-strong positivity vs cisplatin that showed local-weak staining.Conclusion: These findings imply that L-carnitine, by its pleiotropic actions in activating Wnt signaling, alleviates cisplatin-induced renal and hepatic destruction. It might be a method of preventing cisplatin-related nephrotoxicity and hepatotoxicity.https://ijbms.mums.ac.ir/article_20562_e3ca7e8eac303d0c63dc23a0fac4414d.pdfβ-catenincisplatincyclin dhepatic regenerationl-carnitinetubular necrosis |
| spellingShingle | snur Hassan Azad K Saeed Omed Omer Rahim Shler A F Mahmood Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine Iranian Journal of Basic Medical Sciences β-catenin cisplatin cyclin d hepatic regeneration l-carnitine tubular necrosis |
| title | Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine |
| title_full | Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine |
| title_fullStr | Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine |
| title_full_unstemmed | Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine |
| title_short | Alleviation of cisplatin-induced hepatotoxicity and nephrotoxicity by L-carnitine |
| title_sort | alleviation of cisplatin induced hepatotoxicity and nephrotoxicity by l carnitine |
| topic | β-catenin cisplatin cyclin d hepatic regeneration l-carnitine tubular necrosis |
| url | https://ijbms.mums.ac.ir/article_20562_e3ca7e8eac303d0c63dc23a0fac4414d.pdf |
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