Prevalence of Escherichia coli O157:H7 in bovine feces in North West of Iran
The objective of this study was to identify and estimate the prevalence of <em>Escherichia</em> <em>coli</em> O157:H7 in cattle fecal samples in northwest of Iran, using multiplex polymerase chain reaction (PCR). In this study, cattle fecal samples were collected from an abat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-05-01
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| Series: | Microbiology Research |
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| Online Access: | http://www.pagepress.org/journals/index.php/mr/article/view/6587 |
| Summary: | The objective of this study was to identify and estimate the prevalence of <em>Escherichia</em> <em>coli</em> O157:H7 in cattle fecal samples in northwest of Iran, using multiplex polymerase chain reaction (PCR). In this study, cattle fecal samples were collected from an abattoir in Tabriz, Iran. After enrichment, isolation was carried out on CT-SMAC culture and afterwards, the identification of <em>E. coli</em> O157 was achieved on colorless sorbitol negative colonies. Then, one of these colonies was analyzed by PCR to identify genes coding for verotoxin 1 and 2 (<em>vt1</em> and <em>vt2</em>), intimin (<em>eceAO157</em>), and H7 flagella antigen (<em>fliCh7</em>). Of 200 samples, 22 were positive by CT-SMAC culture. Of the 22 sorbitol-negative samples investigated by PCR, two isolates were identified as <em>E. coli</em> O157:H7. Three isolates carried <em>eaeAO157</em> specific <em>locus</em> (non-VTEC <em>E.coli</em> O157) and one or both the verotoxin genes (<em>vtx1</em> and <em>vtx2</em>) only (non-O157 VTEC). Low prevalence rates of<em> E. coli</em> O157:H7 in cattle feces as an important animal reservoir of the mentioned bacterium should be added to the variety of factors mentioned for the low prevalence of <em>E. coli</em> O157:H7 in Iran. |
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| ISSN: | 2036-7473 2036-7481 |