Mesenchymal stem cell spheroids: potential cell materials for cell therapy
Mesenchymal stromal/stem cells (MSCs) have been applied in clinical trials with an increasing number in recent years. MSCs showed their great potentials in regenerative medicine for their extensive sources, multilineage differentiation potential, low immunogenicity and self-renewal ability. However,...
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| Format: | Article |
| Language: | English |
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Innovation Publishing House Pte. Ltd.
2020-12-01
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| Series: | STEMedicine |
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| Online Access: | https://stemedicine.org/index.php/stem/article/view/67 |
| _version_ | 1831646208926416896 |
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| author | Zhongjuan Xu Xingzhi Liu Yu Wei Zhe Zhao Junjun Cao Yong Qiao Yanzhen Yu Junjie Zhong Guangli Suo |
| author_facet | Zhongjuan Xu Xingzhi Liu Yu Wei Zhe Zhao Junjun Cao Yong Qiao Yanzhen Yu Junjie Zhong Guangli Suo |
| author_sort | Zhongjuan Xu |
| collection | DOAJ |
| description | Mesenchymal stromal/stem cells (MSCs) have been applied in clinical trials with an increasing number in recent years. MSCs showed their great potentials in regenerative medicine for their extensive sources, multilineage differentiation potential, low immunogenicity and self-renewal ability. However, the clinical application of MSCs still confronts many challenges including the requirement of large quantity of cells, low survival ability in vivo and the loss of main original characteristics due to two-dimensional (2D) culture although it is beneficial to cells fast expansion. Three-dimensional (3D) culture artificially creates an environment that permits cells to grow or interact with their surroundings in all three dimensions. Therefore, 3D culture was widely regarded as a more preferable and closer physiological microenvironment for cells growth. Recently, many different 3D spheroid culture methods have been developed to optimize MSCs biological characteristics to meet the demand of regenerative medicine. In this review, we comprehensively discussed the merits and demerits of different spheroid formation methods, expounded the mechanisms of spheroid formation and its microenvironment, and illustrated their optimized biological functions and the pre-clinical applications in various tissue injury and regeneration. In the end, we prospected the trends of this research field and proposed the key problems needed to be solved in the future. |
| first_indexed | 2024-12-19T13:50:27Z |
| format | Article |
| id | doaj.art-78afc51d3b3d4752a639348f4a416cf9 |
| institution | Directory Open Access Journal |
| issn | 2705-1188 |
| language | English |
| last_indexed | 2024-12-19T13:50:27Z |
| publishDate | 2020-12-01 |
| publisher | Innovation Publishing House Pte. Ltd. |
| record_format | Article |
| series | STEMedicine |
| spelling | doaj.art-78afc51d3b3d4752a639348f4a416cf92022-12-21T20:18:45ZengInnovation Publishing House Pte. Ltd.STEMedicine2705-11882020-12-012510.37175/stemedicine.v2i5.67Mesenchymal stem cell spheroids: potential cell materials for cell therapyZhongjuan Xu0Xingzhi Liu1Yu Wei2Zhe Zhao3Junjun Cao4Yong Qiao5Yanzhen Yu6Junjie Zhong7Guangli Suo8CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, Anhui 230026, China.CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, Anhui 230026, China.Livingchip Lnc., Nanjing, Jiangsu 211112, China.CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China.CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, Anhui 230026, China. CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China.CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, Anhui 230026, China. Department of Neurosurgery, Fudan University Huashan Hospital, National Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Shanghai Medical College, Fudan University, Shanghai 200041, China.CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Jiangsu 215123, China.Mesenchymal stromal/stem cells (MSCs) have been applied in clinical trials with an increasing number in recent years. MSCs showed their great potentials in regenerative medicine for their extensive sources, multilineage differentiation potential, low immunogenicity and self-renewal ability. However, the clinical application of MSCs still confronts many challenges including the requirement of large quantity of cells, low survival ability in vivo and the loss of main original characteristics due to two-dimensional (2D) culture although it is beneficial to cells fast expansion. Three-dimensional (3D) culture artificially creates an environment that permits cells to grow or interact with their surroundings in all three dimensions. Therefore, 3D culture was widely regarded as a more preferable and closer physiological microenvironment for cells growth. Recently, many different 3D spheroid culture methods have been developed to optimize MSCs biological characteristics to meet the demand of regenerative medicine. In this review, we comprehensively discussed the merits and demerits of different spheroid formation methods, expounded the mechanisms of spheroid formation and its microenvironment, and illustrated their optimized biological functions and the pre-clinical applications in various tissue injury and regeneration. In the end, we prospected the trends of this research field and proposed the key problems needed to be solved in the future.https://stemedicine.org/index.php/stem/article/view/67Mesenchymal stromal/stem cellsThree-dimensional cultureSpheroidsStem cell optimizationRegenerative medicine |
| spellingShingle | Zhongjuan Xu Xingzhi Liu Yu Wei Zhe Zhao Junjun Cao Yong Qiao Yanzhen Yu Junjie Zhong Guangli Suo Mesenchymal stem cell spheroids: potential cell materials for cell therapy STEMedicine Mesenchymal stromal/stem cells Three-dimensional culture Spheroids Stem cell optimization Regenerative medicine |
| title | Mesenchymal stem cell spheroids: potential cell materials for cell therapy |
| title_full | Mesenchymal stem cell spheroids: potential cell materials for cell therapy |
| title_fullStr | Mesenchymal stem cell spheroids: potential cell materials for cell therapy |
| title_full_unstemmed | Mesenchymal stem cell spheroids: potential cell materials for cell therapy |
| title_short | Mesenchymal stem cell spheroids: potential cell materials for cell therapy |
| title_sort | mesenchymal stem cell spheroids potential cell materials for cell therapy |
| topic | Mesenchymal stromal/stem cells Three-dimensional culture Spheroids Stem cell optimization Regenerative medicine |
| url | https://stemedicine.org/index.php/stem/article/view/67 |
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