Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258
<p>Abstract</p> <p>Background</p> <p>Prostate cancer is a frequent finding in man. In dogs, malignant disease of the prostate is also of clinical relevance, although it is a less common diagnosis. Even though there are numerous differences in origin and development of t...
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| Format: | Article |
| Language: | English |
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BMC
2008-08-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/8/240 |
| _version_ | 1818444658374606848 |
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| author | Winkler Susanne Willenbrock Saskia Sterenczak Katharina A Soller Jan T Escobar Hugo Fork Melani AM Dorsch Martina Reimann-Berg Nicola Hedrich Hans J Bullerdiek Jörn Nolte Ingo |
| author_facet | Winkler Susanne Willenbrock Saskia Sterenczak Katharina A Soller Jan T Escobar Hugo Fork Melani AM Dorsch Martina Reimann-Berg Nicola Hedrich Hans J Bullerdiek Jörn Nolte Ingo |
| author_sort | Winkler Susanne |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Prostate cancer is a frequent finding in man. In dogs, malignant disease of the prostate is also of clinical relevance, although it is a less common diagnosis. Even though there are numerous differences in origin and development of the disease, man and dog share many similarities in the pathological presentation. For this reason, the dog might be a useful animal model for prostate malignancies in man.</p> <p>Although prostate cancer is of great importance in veterinary medicine as well as in comparative medicine, there are only few cell lines available. Thus, it was the aim of the present study to determine whether the formerly established prostate carcinoma cell line CT1258 is a suitable tool for <it>in vivo </it>testing, and to distinguish the growth pattern of the induced tumours.</p> <p>Methods</p> <p>For characterisation of the <it>in vivo </it>behaviour of the <it>in vitro </it>established canine prostate carcinoma cell line CT1258, cells were inoculated in 19 NOD.CB17-<it>Prkdc</it><sup><it>Scid</it></sup>/J (in the following: NOD-Scid) mice, either subcutaneously or intraperitoneally. After sacrifice, the obtained specimens were examined histologically and compared to the pattern of the original tumour in the donor.</p> <p>Cytogenetic investigation was performed.</p> <p>Results</p> <p>The cell line CT 1258 not only showed to be highly tumourigenic after subcutaneous as well as intraperitoneal inoculation, but also mimicked the behaviour of the original tumour.</p> <p>Conclusion</p> <p>Tumours induced by inoculation of the cell line CT1258 resemble the situation in naturally occurring prostate carcinoma in the dog, and thus could be used as <it>in vivo </it>model for future studies.</p> |
| first_indexed | 2024-12-14T19:19:26Z |
| format | Article |
| id | doaj.art-78b0157bdc544c51b79f29717d871baa |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-14T19:19:26Z |
| publishDate | 2008-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-78b0157bdc544c51b79f29717d871baa2022-12-21T22:50:23ZengBMCBMC Cancer1471-24072008-08-018124010.1186/1471-2407-8-240Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258Winkler SusanneWillenbrock SaskiaSterenczak Katharina ASoller Jan TEscobar HugoFork Melani AMDorsch MartinaReimann-Berg NicolaHedrich Hans JBullerdiek JörnNolte Ingo<p>Abstract</p> <p>Background</p> <p>Prostate cancer is a frequent finding in man. In dogs, malignant disease of the prostate is also of clinical relevance, although it is a less common diagnosis. Even though there are numerous differences in origin and development of the disease, man and dog share many similarities in the pathological presentation. For this reason, the dog might be a useful animal model for prostate malignancies in man.</p> <p>Although prostate cancer is of great importance in veterinary medicine as well as in comparative medicine, there are only few cell lines available. Thus, it was the aim of the present study to determine whether the formerly established prostate carcinoma cell line CT1258 is a suitable tool for <it>in vivo </it>testing, and to distinguish the growth pattern of the induced tumours.</p> <p>Methods</p> <p>For characterisation of the <it>in vivo </it>behaviour of the <it>in vitro </it>established canine prostate carcinoma cell line CT1258, cells were inoculated in 19 NOD.CB17-<it>Prkdc</it><sup><it>Scid</it></sup>/J (in the following: NOD-Scid) mice, either subcutaneously or intraperitoneally. After sacrifice, the obtained specimens were examined histologically and compared to the pattern of the original tumour in the donor.</p> <p>Cytogenetic investigation was performed.</p> <p>Results</p> <p>The cell line CT 1258 not only showed to be highly tumourigenic after subcutaneous as well as intraperitoneal inoculation, but also mimicked the behaviour of the original tumour.</p> <p>Conclusion</p> <p>Tumours induced by inoculation of the cell line CT1258 resemble the situation in naturally occurring prostate carcinoma in the dog, and thus could be used as <it>in vivo </it>model for future studies.</p>http://www.biomedcentral.com/1471-2407/8/240 |
| spellingShingle | Winkler Susanne Willenbrock Saskia Sterenczak Katharina A Soller Jan T Escobar Hugo Fork Melani AM Dorsch Martina Reimann-Berg Nicola Hedrich Hans J Bullerdiek Jörn Nolte Ingo Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258 BMC Cancer |
| title | Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258 |
| title_full | Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258 |
| title_fullStr | Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258 |
| title_full_unstemmed | Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258 |
| title_short | Establishing an <it>in vivo </it>model of canine prostate carcinoma using the new cell line CT1258 |
| title_sort | establishing an it in vivo it model of canine prostate carcinoma using the new cell line ct1258 |
| url | http://www.biomedcentral.com/1471-2407/8/240 |
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