CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression
Abstract Background An increasing number of studies have shown that circular RNAs (circRNAs) play important roles in the regulation of tumor progression. Therefore, we explored the expression characteristics, function, and related mechanism of the newly identified circNALCN in glioma. Methods RNA se...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-06-01
|
| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-021-02001-y |
| _version_ | 1818864934367264768 |
|---|---|
| author | Yi Liu Simin Chen Gang Peng Yiwei Liao Xuegong Fan Zuping Zhang Chenfu Shen |
| author_facet | Yi Liu Simin Chen Gang Peng Yiwei Liao Xuegong Fan Zuping Zhang Chenfu Shen |
| author_sort | Yi Liu |
| collection | DOAJ |
| description | Abstract Background An increasing number of studies have shown that circular RNAs (circRNAs) play important roles in the regulation of tumor progression. Therefore, we explored the expression characteristics, function, and related mechanism of the newly identified circNALCN in glioma. Methods RNA sequencing was used to analyze the expression profiles of circRNAs in brain tissue from five glioma cases and four normal controls. Quantitative real-time polymerase chain reaction was implemented to examine the levels of circNALCN, miR-493-3p, and phosphatase and tensin homolog (PTEN). Cell counting kit 8 assays were performed to analyze cell proliferation, and cell migration was assessed by the wound healing test and Transwell assay. Dual-luciferase reporter, fluorescence in situ hybridization, and RNA pulldown assays were performed to confirm the role of circNALCN as an miR-493-3p sponge, weakening the inhibitory effect of miR-493-3p on target PTEN expression. Results The downregulated expression of circNALCN was observed in both glioma tissues and cell lines. CircNALCN expression was negatively correlated with World Health Organization grade and overall survival in patients with glioma. Functionally, the overexpression of circNALCN significantly inhibited the proliferation and migration of glioma cells, whereas miR-493-3p mimics counteracted these effects. The mechanistic analysis demonstrated that circNALCN acted as a competing endogenous RNA for miR-493-3p to relieve the repressive effects of miR-493-3p on its target, PTEN, suppressing glioma tumorigenesis. Conclusions CircNALCN inhibits the progression of glioma through the miR-493-3p/PTEN axis, providing a developable biomarker and therapeutic target for glioma patients. |
| first_indexed | 2024-12-19T10:39:32Z |
| format | Article |
| id | doaj.art-78b08dac38e5478d928210b3c1dc6bb2 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-19T10:39:32Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-78b08dac38e5478d928210b3c1dc6bb22022-12-21T20:25:30ZengBMCCancer Cell International1475-28672021-06-0121111810.1186/s12935-021-02001-yCircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progressionYi Liu0Simin Chen1Gang Peng2Yiwei Liao3Xuegong Fan4Zuping Zhang5Chenfu Shen6Department of Neurosurgery, Xiangya Hospital, Central South UniversitySchool of Basic Medicine, Central South UniversityDepartment of Neurosurgery, Xiangya Hospital, Central South UniversityDepartment of Neurosurgery, Xiangya Hospital, Central South UniversityHunan Key Laboratory of Viral Hepatitis, Xiangya Hospital, Central South UniversitySchool of Basic Medicine, Central South UniversityDepartment of Neurosurgery, Xiangya Hospital, Central South UniversityAbstract Background An increasing number of studies have shown that circular RNAs (circRNAs) play important roles in the regulation of tumor progression. Therefore, we explored the expression characteristics, function, and related mechanism of the newly identified circNALCN in glioma. Methods RNA sequencing was used to analyze the expression profiles of circRNAs in brain tissue from five glioma cases and four normal controls. Quantitative real-time polymerase chain reaction was implemented to examine the levels of circNALCN, miR-493-3p, and phosphatase and tensin homolog (PTEN). Cell counting kit 8 assays were performed to analyze cell proliferation, and cell migration was assessed by the wound healing test and Transwell assay. Dual-luciferase reporter, fluorescence in situ hybridization, and RNA pulldown assays were performed to confirm the role of circNALCN as an miR-493-3p sponge, weakening the inhibitory effect of miR-493-3p on target PTEN expression. Results The downregulated expression of circNALCN was observed in both glioma tissues and cell lines. CircNALCN expression was negatively correlated with World Health Organization grade and overall survival in patients with glioma. Functionally, the overexpression of circNALCN significantly inhibited the proliferation and migration of glioma cells, whereas miR-493-3p mimics counteracted these effects. The mechanistic analysis demonstrated that circNALCN acted as a competing endogenous RNA for miR-493-3p to relieve the repressive effects of miR-493-3p on its target, PTEN, suppressing glioma tumorigenesis. Conclusions CircNALCN inhibits the progression of glioma through the miR-493-3p/PTEN axis, providing a developable biomarker and therapeutic target for glioma patients.https://doi.org/10.1186/s12935-021-02001-yGliomaPrognosiscircNALCNmiR-493-3pPTENceRNA |
| spellingShingle | Yi Liu Simin Chen Gang Peng Yiwei Liao Xuegong Fan Zuping Zhang Chenfu Shen CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression Cancer Cell International Glioma Prognosis circNALCN miR-493-3p PTEN ceRNA |
| title | CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression |
| title_full | CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression |
| title_fullStr | CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression |
| title_full_unstemmed | CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression |
| title_short | CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression |
| title_sort | circrna nalcn acts as an mir 493 3p sponge to regulate pten expression and inhibit glioma progression |
| topic | Glioma Prognosis circNALCN miR-493-3p PTEN ceRNA |
| url | https://doi.org/10.1186/s12935-021-02001-y |
| work_keys_str_mv | AT yiliu circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression AT siminchen circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression AT gangpeng circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression AT yiweiliao circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression AT xuegongfan circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression AT zupingzhang circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression AT chenfushen circrnanalcnactsasanmir4933pspongetoregulateptenexpressionandinhibitgliomaprogression |