A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer
Lymph node metastasis (LNM) of colorectal cancer (CRC) is an important factor for both prognosis and treatment. Given the deficiencies of conventional tests, we aim to discover novel DNA methylation markers to efficiently identify LNM status of CRC. In this study, genome-wide methylation sequencing...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.1000823/full |
| _version_ | 1811339685700567040 |
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| author | Yingdian Yu Wenyuan Xue Zefeng Liu Shang Chen Jun Wang Quanzhou Peng Linhao Xu Xin Liu Chunhui Cui Jian-Bing Fan Jian-Bing Fan Jian-Bing Fan |
| author_facet | Yingdian Yu Wenyuan Xue Zefeng Liu Shang Chen Jun Wang Quanzhou Peng Linhao Xu Xin Liu Chunhui Cui Jian-Bing Fan Jian-Bing Fan Jian-Bing Fan |
| author_sort | Yingdian Yu |
| collection | DOAJ |
| description | Lymph node metastasis (LNM) of colorectal cancer (CRC) is an important factor for both prognosis and treatment. Given the deficiencies of conventional tests, we aim to discover novel DNA methylation markers to efficiently identify LNM status of CRC. In this study, genome-wide methylation sequencing was performed in a cohort (n=30) using fresh CRC tissue to discover differentially methylated markers. These markers were subsequently validated with fluorescence quantitative PCR in a cohort (n=221), and the optimal marker was compared to conventional diagnostic methods. Meanwhile, immunohistochemistry was used to verify the effectiveness of the antibody corresponding to this marker in a cohort (n=56). LBX2 achieved an AUC of 0.87, specificity of 87.3%, sensitivity of 75.7%, and accuracy of 81.9%, which outperformed conventional methods including imaging (CT, PET-CT) with an AUC of 0.52, CA199 with an AUC of 0.58, CEA with an AUC of 0.56. LBX2 was also superior to clinicopathological indicators including the depth of tumor invasion and lymphatic invasion with an AUC of 0.61and 0.63 respectively. Moreover, the AUC of LBX2 antibody was 0.84, which was also better than these conventional methods. In conclusion, A novel methylation marker LBX2 could be used as a simple, cost-effective, and reliable diagnostic method for LNM of CRC. |
| first_indexed | 2024-04-13T18:30:28Z |
| format | Article |
| id | doaj.art-78b1649cfc4a4ae6b5230a3221d98668 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-13T18:30:28Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-78b1649cfc4a4ae6b5230a3221d986682022-12-22T02:35:06ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-10-011210.3389/fonc.2022.10008231000823A novel DNA methylation marker to identify lymph node metastasis of colorectal cancerYingdian Yu0Wenyuan Xue1Zefeng Liu2Shang Chen3Jun Wang4Quanzhou Peng5Linhao Xu6Xin Liu7Chunhui Cui8Jian-Bing Fan9Jian-Bing Fan10Jian-Bing Fan11Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of General Surgery, Zhujiang Hosipital, Southern Medical University, Guangzhou, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaAnchorDx Medical Co., Ltd., International Bio-Island, Guangzhou, ChinaDepartment of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaAnchorDx Medical Co., Ltd., International Bio-Island, Guangzhou, ChinaAnchorDx Medical Co., Ltd., International Bio-Island, Guangzhou, ChinaDepartment of General Surgery, Zhujiang Hosipital, Southern Medical University, Guangzhou, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaAnchorDx Medical Co., Ltd., International Bio-Island, Guangzhou, ChinaDepartment of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaLymph node metastasis (LNM) of colorectal cancer (CRC) is an important factor for both prognosis and treatment. Given the deficiencies of conventional tests, we aim to discover novel DNA methylation markers to efficiently identify LNM status of CRC. In this study, genome-wide methylation sequencing was performed in a cohort (n=30) using fresh CRC tissue to discover differentially methylated markers. These markers were subsequently validated with fluorescence quantitative PCR in a cohort (n=221), and the optimal marker was compared to conventional diagnostic methods. Meanwhile, immunohistochemistry was used to verify the effectiveness of the antibody corresponding to this marker in a cohort (n=56). LBX2 achieved an AUC of 0.87, specificity of 87.3%, sensitivity of 75.7%, and accuracy of 81.9%, which outperformed conventional methods including imaging (CT, PET-CT) with an AUC of 0.52, CA199 with an AUC of 0.58, CEA with an AUC of 0.56. LBX2 was also superior to clinicopathological indicators including the depth of tumor invasion and lymphatic invasion with an AUC of 0.61and 0.63 respectively. Moreover, the AUC of LBX2 antibody was 0.84, which was also better than these conventional methods. In conclusion, A novel methylation marker LBX2 could be used as a simple, cost-effective, and reliable diagnostic method for LNM of CRC.https://www.frontiersin.org/articles/10.3389/fonc.2022.1000823/fullcolorectal cancerlymph node metastasisDNA methylation markerimmunohistochemistrydiagnostic method |
| spellingShingle | Yingdian Yu Wenyuan Xue Zefeng Liu Shang Chen Jun Wang Quanzhou Peng Linhao Xu Xin Liu Chunhui Cui Jian-Bing Fan Jian-Bing Fan Jian-Bing Fan A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer Frontiers in Oncology colorectal cancer lymph node metastasis DNA methylation marker immunohistochemistry diagnostic method |
| title | A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer |
| title_full | A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer |
| title_fullStr | A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer |
| title_full_unstemmed | A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer |
| title_short | A novel DNA methylation marker to identify lymph node metastasis of colorectal cancer |
| title_sort | novel dna methylation marker to identify lymph node metastasis of colorectal cancer |
| topic | colorectal cancer lymph node metastasis DNA methylation marker immunohistochemistry diagnostic method |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.1000823/full |
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