Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum
One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003–2005) and 46 additional isolates were genotyped by internal transcribed spacer–restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2008-02-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/14/2/07-0920_article |
| _version_ | 1828463499727077376 |
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| author | Laurence Delhaes Azian Harun Sharon C.A. Chen Quoc Nguyen Monica Slavin Christopher H. Heath Krystyna Maszewska Catriona Halliday Vincent Robert Tania C. Sorrell Wieland Meyer |
| author_facet | Laurence Delhaes Azian Harun Sharon C.A. Chen Quoc Nguyen Monica Slavin Christopher H. Heath Krystyna Maszewska Catriona Halliday Vincent Robert Tania C. Sorrell Wieland Meyer |
| author_sort | Laurence Delhaes |
| collection | DOAJ |
| description | One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003–2005) and 46 additional isolates were genotyped by internal transcribed spacer–restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed. |
| first_indexed | 2024-12-11T02:55:56Z |
| format | Article |
| id | doaj.art-78b69f977ba14e3eb3f7ea6fbf249c0f |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-11T02:55:56Z |
| publishDate | 2008-02-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-78b69f977ba14e3eb3f7ea6fbf249c0f2022-12-22T01:23:10ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592008-02-0114228229010.3201/eid1402.070920Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacumLaurence DelhaesAzian HarunSharon C.A. ChenQuoc NguyenMonica SlavinChristopher H. HeathKrystyna MaszewskaCatriona HallidayVincent RobertTania C. SorrellWieland MeyerOne hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003–2005) and 46 additional isolates were genotyped by internal transcribed spacer–restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed.https://wwwnc.cdc.gov/eid/article/14/2/07-0920_articleScedosporium prolificansScedosporium aurantiacumScedosporium apiospermummolecular epidemiologyITS-sequencingITS-RFLP |
| spellingShingle | Laurence Delhaes Azian Harun Sharon C.A. Chen Quoc Nguyen Monica Slavin Christopher H. Heath Krystyna Maszewska Catriona Halliday Vincent Robert Tania C. Sorrell Wieland Meyer Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum Emerging Infectious Diseases Scedosporium prolificans Scedosporium aurantiacum Scedosporium apiospermum molecular epidemiology ITS-sequencing ITS-RFLP |
| title | Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum |
| title_full | Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum |
| title_fullStr | Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum |
| title_full_unstemmed | Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum |
| title_short | Molecular Typing of Australian Scedosporium Isolates Showing Genetic Variability and Numerous S. aurantiacum |
| title_sort | molecular typing of australian scedosporium isolates showing genetic variability and numerous s aurantiacum |
| topic | Scedosporium prolificans Scedosporium aurantiacum Scedosporium apiospermum molecular epidemiology ITS-sequencing ITS-RFLP |
| url | https://wwwnc.cdc.gov/eid/article/14/2/07-0920_article |
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