Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin
Due to its ability to lower cholesterol levels, simvastatin is a leading drug for the prevention of strokes and heart disease: it also lowers the incidence of neurodegenerative diseases. Simvastatin is made from lovastatin, a precursor produced by the industrial fungus, <i>Aspergillus terreus&...
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MDPI AG
2020-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/7/3/101 |
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| author | Mishal Subhan Rani Faryal Ian Macreadie |
| author_facet | Mishal Subhan Rani Faryal Ian Macreadie |
| author_sort | Mishal Subhan |
| collection | DOAJ |
| description | Due to its ability to lower cholesterol levels, simvastatin is a leading drug for the prevention of strokes and heart disease: it also lowers the incidence of neurodegenerative diseases. Simvastatin is made from lovastatin, a precursor produced by the industrial fungus, <i>Aspergillus terreus</i>. In this study, <i>Corymbia maculata</i> leaves were tested as a novel substrate for the growth of a new isolate of <i>A. terreus</i> and a lovastatin-resistant strain of <i>A. terreus</i> to produce lovastatin. <i>Corymbia maculata</i> (spotted gum) is well utilized by forest industries as a source of timber because of its high strength, durability and smooth texture. However, the leaves are a major waste product. Growth of <i>A. terreus</i> on <i>Corymbia maculata</i> leaves, in solid-state fermentation resulted in the production of lovastatin. Fermentation of media using fresh leaves of <i>Corymbia maculata</i> produced more lovastatin (4.9 mg g<sup>−1</sup>), than the sun-dried leaves (3.2 mg g<sup>−1</sup>). Levels of lovastatin were further increased by the lovastatin-resistant strain of <i>A. terreus</i> (Lvs-r), which produced twice the amount of the parental strain. The production of lovastatin was confirmed by HPLC and LC–MS/MS studies. The study suggests that the utilization of a cheap substrate for the production of lovastatin can have a potential economic benefit. |
| first_indexed | 2024-03-10T16:42:57Z |
| format | Article |
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| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T16:42:57Z |
| publishDate | 2020-08-01 |
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| series | Bioengineering |
| spelling | doaj.art-78b7f4ea21c54e0681080d6fe7653ca52023-11-20T11:52:55ZengMDPI AGBioengineering2306-53542020-08-017310110.3390/bioengineering7030101Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce LovastatinMishal Subhan0Rani Faryal1Ian Macreadie2Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanSchool of Science, RMIT University, Bundoora, VIC 3083, AustraliaDue to its ability to lower cholesterol levels, simvastatin is a leading drug for the prevention of strokes and heart disease: it also lowers the incidence of neurodegenerative diseases. Simvastatin is made from lovastatin, a precursor produced by the industrial fungus, <i>Aspergillus terreus</i>. In this study, <i>Corymbia maculata</i> leaves were tested as a novel substrate for the growth of a new isolate of <i>A. terreus</i> and a lovastatin-resistant strain of <i>A. terreus</i> to produce lovastatin. <i>Corymbia maculata</i> (spotted gum) is well utilized by forest industries as a source of timber because of its high strength, durability and smooth texture. However, the leaves are a major waste product. Growth of <i>A. terreus</i> on <i>Corymbia maculata</i> leaves, in solid-state fermentation resulted in the production of lovastatin. Fermentation of media using fresh leaves of <i>Corymbia maculata</i> produced more lovastatin (4.9 mg g<sup>−1</sup>), than the sun-dried leaves (3.2 mg g<sup>−1</sup>). Levels of lovastatin were further increased by the lovastatin-resistant strain of <i>A. terreus</i> (Lvs-r), which produced twice the amount of the parental strain. The production of lovastatin was confirmed by HPLC and LC–MS/MS studies. The study suggests that the utilization of a cheap substrate for the production of lovastatin can have a potential economic benefit.https://www.mdpi.com/2306-5354/7/3/101<i>Aspergillus terreus</i>eucalyptusfermentationlovastatinSmFSSF |
| spellingShingle | Mishal Subhan Rani Faryal Ian Macreadie Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin Bioengineering <i>Aspergillus terreus</i> eucalyptus fermentation lovastatin SmF SSF |
| title | Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin |
| title_full | Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin |
| title_fullStr | Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin |
| title_full_unstemmed | Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin |
| title_short | Utilization of an Industry Byproduct, <i>Corymbia maculata</i> Leaves, by <i>Aspergillus terreus</i> to Produce Lovastatin |
| title_sort | utilization of an industry byproduct i corymbia maculata i leaves by i aspergillus terreus i to produce lovastatin |
| topic | <i>Aspergillus terreus</i> eucalyptus fermentation lovastatin SmF SSF |
| url | https://www.mdpi.com/2306-5354/7/3/101 |
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