Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways
Objective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2015-05-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_4411_ad5a6a1395a2b6c183cad6f1e52d6a60.html |
| _version_ | 1818063588193992704 |
|---|---|
| author | Wen-Chieh Lin Yu-Fen Peng Chien-Wei Hou |
| author_facet | Wen-Chieh Lin Yu-Fen Peng Chien-Wei Hou |
| author_sort | Wen-Chieh Lin |
| collection | DOAJ |
| description | Objective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. Results:FA increased cell viability, prevented membrane damage (LDH release), scavenged free radicals, increased superoxide dismutase (SOD) activity, and attenuated the elevation of intracellular free Ca2+, lipid peroxidation, apoptosis (evaluated by TUNEL staining) and PGE2 production in hypoxia-stressed PC12 cells. MAPKs were activated during hypoxia. FA reduced p-p38 MAPK, caspase-3, and COX-2 activation which correlated well with diminished LDH release in PC12 cells under hypoxia. Furthermore, FA reduced lipid peroxidation in PC12 cells subjected to hypoxia. Conclusion:Taken together, these results indicate that FA antioxidant effects could partly be involved in inhibition of p38 MAPK pathway and apoptosis through scavenging ROS in hypoxia-stressed PC12 cells. |
| first_indexed | 2024-12-10T14:22:29Z |
| format | Article |
| id | doaj.art-78b80b67f3404de0855742aac4b49638 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-10T14:22:29Z |
| publishDate | 2015-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-78b80b67f3404de0855742aac4b496382022-12-22T01:45:10ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-05-011854784844411Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathwaysWen-Chieh Lin0Yu-Fen Peng1Chien-Wei Hou2Department of Optometry, Yuanpei University, Hsinchu, TaiwanDepartment of Physical Education Office, Yuanpei University, Hsinchu, TaiwanDepartment of Biotechnology, Yuanpei University, Hsinchu, TaiwanObjective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. Results:FA increased cell viability, prevented membrane damage (LDH release), scavenged free radicals, increased superoxide dismutase (SOD) activity, and attenuated the elevation of intracellular free Ca2+, lipid peroxidation, apoptosis (evaluated by TUNEL staining) and PGE2 production in hypoxia-stressed PC12 cells. MAPKs were activated during hypoxia. FA reduced p-p38 MAPK, caspase-3, and COX-2 activation which correlated well with diminished LDH release in PC12 cells under hypoxia. Furthermore, FA reduced lipid peroxidation in PC12 cells subjected to hypoxia. Conclusion:Taken together, these results indicate that FA antioxidant effects could partly be involved in inhibition of p38 MAPK pathway and apoptosis through scavenging ROS in hypoxia-stressed PC12 cells.http://ijbms.mums.ac.ir/pdf_4411_ad5a6a1395a2b6c183cad6f1e52d6a60.htmlCaspase-3Ferulic acidHypoxiaMAPKsROS |
| spellingShingle | Wen-Chieh Lin Yu-Fen Peng Chien-Wei Hou Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways Iranian Journal of Basic Medical Sciences Caspase-3 Ferulic acid Hypoxia MAPKs ROS |
| title | Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways |
| title_full | Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways |
| title_fullStr | Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways |
| title_full_unstemmed | Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways |
| title_short | Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways |
| title_sort | ferulic acid protects pc12 neurons against hypoxia by inhibiting the p mapks and cox 2 pathways |
| topic | Caspase-3 Ferulic acid Hypoxia MAPKs ROS |
| url | http://ijbms.mums.ac.ir/pdf_4411_ad5a6a1395a2b6c183cad6f1e52d6a60.html |
| work_keys_str_mv | AT wenchiehlin ferulicacidprotectspc12neuronsagainsthypoxiabyinhibitingthepmapksandcox2pathways AT yufenpeng ferulicacidprotectspc12neuronsagainsthypoxiabyinhibitingthepmapksandcox2pathways AT chienweihou ferulicacidprotectspc12neuronsagainsthypoxiabyinhibitingthepmapksandcox2pathways |