Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum
Soybean [Glycine max (L.)], one of the most important crops in Argentina, is commonly infected by Colletotrichum truncatum, the causal agent of anthracnose. Tagetes filifolia essential oil (EO) is presented as a natural approach to minimize the dose of chemical fungicides applied to the crop. The...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2020-03-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2020.132202 |
| _version_ | 1819290657844363264 |
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| author | Laura Carolina Gadban Boris Xavier Camiletti Ezequiel Darío Bigatton Silvia Graciela Distéfano Enrique Iván Lucini |
| author_facet | Laura Carolina Gadban Boris Xavier Camiletti Ezequiel Darío Bigatton Silvia Graciela Distéfano Enrique Iván Lucini |
| author_sort | Laura Carolina Gadban |
| collection | DOAJ |
| description | Soybean [Glycine max (L.)], one of the most important crops in Argentina, is commonly
infected by Colletotrichum truncatum, the causal agent of anthracnose. Tagetes filifolia essential
oil (EO) is presented as a natural approach to minimize the dose of chemical fungicides
applied to the crop. The fungus Trichoderma harzianum is used as a biocontrol
agent because of its ability to produce secondary metabolites that destroy cell walls of phytopathogenic
fungi. However, its performance can be affected when it is exposed to chemical
fungicides. The objective of this work was to evaluate the antifungal activity of T. filifolia
EO both individually and combined with chemical fungicides against C. truncatum, and its
effect on T. harzianum. Fungi were isolated from soybean crops. The following pesticides
were assessed: carbendazim (F1), difenoconazole (F2) and trifloxystrobin + cyproconazole
(F3). The EO was obtained from native plants and its chemical composition was analyzed
by gas chromatography–mass spectrometry (GC–MS). The minimum fungicide concentration
(MFC) was determined for each compound. Fungicides were combined with the EO
to look for combinations that allowed a reduction of pesticide doses. Among fungicides,
F1 showed the strongest antifungal activity against C. truncatum (MFC = 0.25 μl ⋅ l–1) and
T. harzianum (MFC = 1.5 μl ⋅ l–1). The sensitivity of both fungi to the EO was lower than
to fungicides. The EO presented MFCs of 6,000 and 9,000 μl ⋅ l–1 against C. truncatum and
T. harzianum. The EO and F1 affected the growth of T. harzianum at concentrations that
controlled C. truncatum (31 and 10%). Eight combinations of fungicides and the EO allowed
fungicide concentration reductions of up to 80%, although the growth of the biocontrol
strain was also affected. The results demonstrated that T. filifolia EO can be used to control
anthracnose and reduce doses of chemical fungicides applied to soybean crops. Its effect on
T. harzianum should be considered in the design of integrated pest management strategies. |
| first_indexed | 2024-12-24T03:26:14Z |
| format | Article |
| id | doaj.art-78b8191313ec4520aa815dce98b02e3b |
| institution | Directory Open Access Journal |
| issn | 1899-007X 1899-007X |
| language | English |
| last_indexed | 2024-12-24T03:26:14Z |
| publishDate | 2020-03-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-78b8191313ec4520aa815dce98b02e3b2022-12-21T17:17:20ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2020-03-01601415010.24425/jppr.2020.132202Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianumLaura Carolina Gadban0 Boris Xavier Camiletti 1https://orcid.org/0000-0002-1492-7988Ezequiel Darío Bigatton2Silvia Graciela Distéfano3Enrique Iván Lucini4Estacioń Experimental Agropecuaria Marcos Juárez/Laboratorio de Fitopatologia, Instituto Nacional de Tecnología Agropecuaria (INTA), Córdoba, ArgentinaMicrobiologia Agricola, Facultad de Ciencias Agropecuarias-Universidad nacional de Cordoba (UNC), Córdoba, Argentina; Instituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, ArgentinaMicrobiologia Agricola, Facultad de Ciencias Agropecuarias-Universidad nacional de Cordoba (UNC), Córdoba, ArgentinaEstacioń Experimental Agropecuaria Marcos Juárez/Laboratorio de Fitopatologia, Instituto Nacional de Tecnología Agropecuaria (INTA), Córdoba, ArgentinaMicrobiologia Agricola, Facultad de Ciencias Agropecuarias-Universidad nacional de Cordoba (UNC), Córdoba, ArgentinaSoybean [Glycine max (L.)], one of the most important crops in Argentina, is commonly infected by Colletotrichum truncatum, the causal agent of anthracnose. Tagetes filifolia essential oil (EO) is presented as a natural approach to minimize the dose of chemical fungicides applied to the crop. The fungus Trichoderma harzianum is used as a biocontrol agent because of its ability to produce secondary metabolites that destroy cell walls of phytopathogenic fungi. However, its performance can be affected when it is exposed to chemical fungicides. The objective of this work was to evaluate the antifungal activity of T. filifolia EO both individually and combined with chemical fungicides against C. truncatum, and its effect on T. harzianum. Fungi were isolated from soybean crops. The following pesticides were assessed: carbendazim (F1), difenoconazole (F2) and trifloxystrobin + cyproconazole (F3). The EO was obtained from native plants and its chemical composition was analyzed by gas chromatography–mass spectrometry (GC–MS). The minimum fungicide concentration (MFC) was determined for each compound. Fungicides were combined with the EO to look for combinations that allowed a reduction of pesticide doses. Among fungicides, F1 showed the strongest antifungal activity against C. truncatum (MFC = 0.25 μl ⋅ l–1) and T. harzianum (MFC = 1.5 μl ⋅ l–1). The sensitivity of both fungi to the EO was lower than to fungicides. The EO presented MFCs of 6,000 and 9,000 μl ⋅ l–1 against C. truncatum and T. harzianum. The EO and F1 affected the growth of T. harzianum at concentrations that controlled C. truncatum (31 and 10%). Eight combinations of fungicides and the EO allowed fungicide concentration reductions of up to 80%, although the growth of the biocontrol strain was also affected. The results demonstrated that T. filifolia EO can be used to control anthracnose and reduce doses of chemical fungicides applied to soybean crops. Its effect on T. harzianum should be considered in the design of integrated pest management strategies.https://doi.org/10.24425/jppr.2020.132202anthracnoseintegrated pest managementnatural controlsoybean |
| spellingShingle | Laura Carolina Gadban Boris Xavier Camiletti Ezequiel Darío Bigatton Silvia Graciela Distéfano Enrique Iván Lucini Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum Journal of Plant Protection Research anthracnose integrated pest management natural control soybean |
| title | Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum |
| title_full | Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum |
| title_fullStr | Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum |
| title_full_unstemmed | Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum |
| title_short | Combinations of Tagetes filifolia Lag. essential oil with chemical fungicides to control Colletotrichum truncatum and their effects on the biocontrol agent Trichoderma harzianum |
| title_sort | combinations of tagetes filifolia lag essential oil with chemical fungicides to control colletotrichum truncatum and their effects on the biocontrol agent trichoderma harzianum |
| topic | anthracnose integrated pest management natural control soybean |
| url | https://doi.org/10.24425/jppr.2020.132202 |
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