Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo
Ovothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of...
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| Format: | Article |
| Language: | English |
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The Royal Society
2022-01-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.210262 |
| _version_ | 1827935230396203008 |
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| author | Alfonsina Milito Maria Cocurullo Alfredo Columbro Simona Nonnis Gabriella Tedeschi Immacolata Castellano Maria Ina Arnone Anna Palumbo |
| author_facet | Alfonsina Milito Maria Cocurullo Alfredo Columbro Simona Nonnis Gabriella Tedeschi Immacolata Castellano Maria Ina Arnone Anna Palumbo |
| author_sort | Alfonsina Milito |
| collection | DOAJ |
| description | Ovothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of the sea urchin Paracentrotus lividus by assessing the localization of the key biosynthetic enzyme OvoA, both at transcript and protein level, and perturbing its protein translation by morpholino antisense oligonucleotide-mediated knockdown experiments. In addition, we explored the possible involvement of ovothiol in the inflammatory response by assessing ovoA gene expression and protein localization following exposure to bacterial lipopolysaccharide. The results of the present study suggest that ovothiol may be a key regulator of cell proliferation in early developing embryos. Moreover, the localization of OvoA in key larval cells and tissues, in control and inflammatory conditions, suggests that ovothiol may ensure larval skeleton formation and mediate inflammatory processes triggered by bacterial infection. This work significantly contributes to the understanding of the biological function of ovothiols in marine organisms, and may provide new inspiration for the identification of the biological activities of ovothiols in humans, considering the pharmacological potential of these molecules. |
| first_indexed | 2024-03-13T07:51:07Z |
| format | Article |
| id | doaj.art-78b988de56c546dca4f6d53cb65f6ce6 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-03-13T07:51:07Z |
| publishDate | 2022-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-78b988de56c546dca4f6d53cb65f6ce62023-06-02T16:06:13ZengThe Royal SocietyOpen Biology2046-24412022-01-0112110.1098/rsob.210262Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryoAlfonsina Milito0Maria Cocurullo1Alfredo Columbro2Simona Nonnis3Gabriella Tedeschi4Immacolata Castellano5Maria Ina Arnone6Anna Palumbo7Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, ItalyDepartment of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, Milan, ItalyDepartment of Veterinary Medicine (DIMEVET), Università degli Studi di Milano, Milan, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Naples, ItalyOvothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of the sea urchin Paracentrotus lividus by assessing the localization of the key biosynthetic enzyme OvoA, both at transcript and protein level, and perturbing its protein translation by morpholino antisense oligonucleotide-mediated knockdown experiments. In addition, we explored the possible involvement of ovothiol in the inflammatory response by assessing ovoA gene expression and protein localization following exposure to bacterial lipopolysaccharide. The results of the present study suggest that ovothiol may be a key regulator of cell proliferation in early developing embryos. Moreover, the localization of OvoA in key larval cells and tissues, in control and inflammatory conditions, suggests that ovothiol may ensure larval skeleton formation and mediate inflammatory processes triggered by bacterial infection. This work significantly contributes to the understanding of the biological function of ovothiols in marine organisms, and may provide new inspiration for the identification of the biological activities of ovothiols in humans, considering the pharmacological potential of these molecules.https://royalsocietypublishing.org/doi/10.1098/rsob.210262ovothioloxidative stresscell proliferationsea urchinembryonic developmentmarine organisms |
| spellingShingle | Alfonsina Milito Maria Cocurullo Alfredo Columbro Simona Nonnis Gabriella Tedeschi Immacolata Castellano Maria Ina Arnone Anna Palumbo Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo Open Biology ovothiol oxidative stress cell proliferation sea urchin embryonic development marine organisms |
| title | Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_full | Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_fullStr | Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_full_unstemmed | Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_short | Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo |
| title_sort | ovothiol ensures the correct developmental programme of the sea urchin paracentrotus lividus embryo |
| topic | ovothiol oxidative stress cell proliferation sea urchin embryonic development marine organisms |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.210262 |
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