Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies
IntroductionThe apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.MethodsTo advance our und...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1271731/full |
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| author | Teresa Cruz-Bustos Marlies Dolezal Anna Sophia Feix Bärbel Ruttkowski Karin Hummel Ebrahim Razzazi-Fazeli Anja Joachim |
| author_facet | Teresa Cruz-Bustos Marlies Dolezal Anna Sophia Feix Bärbel Ruttkowski Karin Hummel Ebrahim Razzazi-Fazeli Anja Joachim |
| author_sort | Teresa Cruz-Bustos |
| collection | DOAJ |
| description | IntroductionThe apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.MethodsTo advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis.ResultsA total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production.DiscussionThese findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages. |
| first_indexed | 2024-03-11T15:46:06Z |
| format | Article |
| id | doaj.art-78bda825affe4e55a29519d7750a469f |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T15:46:06Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-78bda825affe4e55a29519d7750a469f2023-10-26T06:17:00ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-10-011310.3389/fcimb.2023.12717311271731Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studiesTeresa Cruz-Bustos0Marlies Dolezal1Anna Sophia Feix2Bärbel Ruttkowski3Karin Hummel4Ebrahim Razzazi-Fazeli5Anja Joachim6Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, AustriaPlatform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, AustriaDepartment of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, AustriaDepartment of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, AustriaVetCore Facility (Proteomics), University of Veterinary Medicine Vienna, Vienna, AustriaVetCore Facility (Proteomics), University of Veterinary Medicine Vienna, Vienna, AustriaDepartment of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, AustriaIntroductionThe apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.MethodsTo advance our understanding of the parasite’s cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis.ResultsA total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production.DiscussionThese findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1271731/fullIsospora suiscoccidiaApicomplexasexual developmentproteomemetabolism |
| spellingShingle | Teresa Cruz-Bustos Marlies Dolezal Anna Sophia Feix Bärbel Ruttkowski Karin Hummel Ebrahim Razzazi-Fazeli Anja Joachim Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies Frontiers in Cellular and Infection Microbiology Isospora suis coccidia Apicomplexa sexual development proteome metabolism |
| title | Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies |
| title_full | Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies |
| title_fullStr | Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies |
| title_full_unstemmed | Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies |
| title_short | Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies |
| title_sort | unravelling the sexual developmental biology of cystoisospora suis a model for comparative coccidian parasite studies |
| topic | Isospora suis coccidia Apicomplexa sexual development proteome metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1271731/full |
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