Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection
Alveolar macrophages (AM) are long-lived tissue-resident innate immune cells of the airways. AM are key effectors of recognition, initiation, and resolution of the host defense against microbes and play an essential role in mediating host responses to <i>Streptococcus pneumoniae</i> infe...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/4/570 |
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| author | Soo Jung Cho Alexander Pronko Jianjun Yang Kassandra Pagan Heather Stout-Delgado |
| author_facet | Soo Jung Cho Alexander Pronko Jianjun Yang Kassandra Pagan Heather Stout-Delgado |
| author_sort | Soo Jung Cho |
| collection | DOAJ |
| description | Alveolar macrophages (AM) are long-lived tissue-resident innate immune cells of the airways. AM are key effectors of recognition, initiation, and resolution of the host defense against microbes and play an essential role in mediating host responses to <i>Streptococcus pneumoniae</i> infection. Lipid metabolism in AM can significantly impact cellular function and biology. Dysregulated metabolism contributes to an accumulation of lipids, unfolded protein response induction, and inflammatory cytokine production. Our study was designed to investigate the impact of Ch25h on mediating innate immune responses by macrophages during <i>S. pneumoniae infection</i>. Using wild-type and <i>Ch25<sup>−/−</sup></i> mice, we examined the role of cholesterol metabolism on inflammatory cytokine production and bacterial clearance. Our results demonstrate that Ch25h plays an important role in the initiation and intensity of cytokine and chemokine production in the lung during <i>S. pneumoniae</i> infection. In the absence of Ch25h, there was enhanced phagocytosis and bacterial clearance. Taken together, our findings demonstrate the important role of Ch25h in modulating host responsiveness to <i>S. pneumoniae</i> infection. |
| first_indexed | 2024-03-11T09:00:57Z |
| format | Article |
| id | doaj.art-78c13fbeb4b644afa2ee1b2c14f934c6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T09:00:57Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-78c13fbeb4b644afa2ee1b2c14f934c62023-11-16T19:44:10ZengMDPI AGCells2073-44092023-02-0112457010.3390/cells12040570Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> InfectionSoo Jung Cho0Alexander Pronko1Jianjun Yang2Kassandra Pagan3Heather Stout-Delgado4Weill Cornell Medicine, New York, NY 10021, USAWeill Cornell Medicine, New York, NY 10021, USAWeill Cornell Medicine, New York, NY 10021, USAWeill Cornell Medicine, New York, NY 10021, USAWeill Cornell Medicine, New York, NY 10021, USAAlveolar macrophages (AM) are long-lived tissue-resident innate immune cells of the airways. AM are key effectors of recognition, initiation, and resolution of the host defense against microbes and play an essential role in mediating host responses to <i>Streptococcus pneumoniae</i> infection. Lipid metabolism in AM can significantly impact cellular function and biology. Dysregulated metabolism contributes to an accumulation of lipids, unfolded protein response induction, and inflammatory cytokine production. Our study was designed to investigate the impact of Ch25h on mediating innate immune responses by macrophages during <i>S. pneumoniae infection</i>. Using wild-type and <i>Ch25<sup>−/−</sup></i> mice, we examined the role of cholesterol metabolism on inflammatory cytokine production and bacterial clearance. Our results demonstrate that Ch25h plays an important role in the initiation and intensity of cytokine and chemokine production in the lung during <i>S. pneumoniae</i> infection. In the absence of Ch25h, there was enhanced phagocytosis and bacterial clearance. Taken together, our findings demonstrate the important role of Ch25h in modulating host responsiveness to <i>S. pneumoniae</i> infection.https://www.mdpi.com/2073-4409/12/4/570<i>Streptococcus pneumoniae</i>Ch25halveolar macrophage |
| spellingShingle | Soo Jung Cho Alexander Pronko Jianjun Yang Kassandra Pagan Heather Stout-Delgado Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection Cells <i>Streptococcus pneumoniae</i> Ch25h alveolar macrophage |
| title | Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection |
| title_full | Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection |
| title_fullStr | Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection |
| title_full_unstemmed | Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection |
| title_short | Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to <i>Streptococcus pneumoniae</i> Infection |
| title_sort | role of cholesterol 25 hydroxylase ch25h in mediating innate immune responses to i streptococcus pneumoniae i infection |
| topic | <i>Streptococcus pneumoniae</i> Ch25h alveolar macrophage |
| url | https://www.mdpi.com/2073-4409/12/4/570 |
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