The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study
Background/purpose: MTA is used to induce hard tissue regeneration in various procedures. This study evaluated the biocompatibility and mineralization potential of mineral trioxide aggregate (MTA) containing calcium fluoride (CaF2). To verify if the change of components affected physical properties,...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Journal of Dental Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790221000994 |
| _version_ | 1818690324391788544 |
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| author | Miyoung Lim Minju Song Chan-Ui Hong Yong-bum Cho |
| author_facet | Miyoung Lim Minju Song Chan-Ui Hong Yong-bum Cho |
| author_sort | Miyoung Lim |
| collection | DOAJ |
| description | Background/purpose: MTA is used to induce hard tissue regeneration in various procedures. This study evaluated the biocompatibility and mineralization potential of mineral trioxide aggregate (MTA) containing calcium fluoride (CaF2). To verify if the change of components affected physical properties, the setting time, solubility, and surface roughness were measured. Materials and methods: Human dental pulp cells (HDPCs) were treated with powder and set MTA containing CaF2 (0, 1, 5, and 10 wt %). The proliferation of HDPCs was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The mineralization potential of HDPCs was investigated with the relative gene expression of alkaline phosphatase (ALP), collagen type I (ColI), osteocalcin (OCN), and runt-related transcription factor 2 (Runx2) using real-time reverse transcription polymerase chain reaction (RT-PCR). For investigating the physical properties, setting time and solubility were tested. Surface profiles of material were analyzed by a non-contact surface profiler and a scanning electron microscope (SEM). Results: MTA-5% CaF2 mixtures increased the proliferation and the mineralization-related gene expression of HDPCs to a greater degree than pure MTA. The addition of CaF2 to MTA delayed the setting, but the difference was only significant in the MTA-10% CaF2. Solubility and surface roughness was not altered. Conclusion: The addition of more than 5% CaF2 can be considered to increase the regeneration potential of pulp cells without adverse effects on physical property. |
| first_indexed | 2024-12-17T12:24:11Z |
| format | Article |
| id | doaj.art-78c37bf1e9d241c79b063bd56da63cb9 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-17T12:24:11Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-78c37bf1e9d241c79b063bd56da63cb92022-12-21T21:48:51ZengElsevierJournal of Dental Sciences1991-79022021-10-0116410801086The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro studyMiyoung Lim0Minju Song1Chan-Ui Hong2Yong-bum Cho3Department of Conservative Dentistry, Dankook University College of Dentistry Jukjeon Hospital, Yongin, South KoreaDepartment of Conservative Dentistry, College of Dentistry, Dankook University, Cheonan, South KoreaDepartment of Conservative Dentistry, College of Dentistry, Dankook University, Cheonan, South KoreaDepartment of Conservative Dentistry, College of Dentistry, Dankook University, Cheonan, South Korea; Corresponding author. Department of Conservative Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, 31116, South Korea.Background/purpose: MTA is used to induce hard tissue regeneration in various procedures. This study evaluated the biocompatibility and mineralization potential of mineral trioxide aggregate (MTA) containing calcium fluoride (CaF2). To verify if the change of components affected physical properties, the setting time, solubility, and surface roughness were measured. Materials and methods: Human dental pulp cells (HDPCs) were treated with powder and set MTA containing CaF2 (0, 1, 5, and 10 wt %). The proliferation of HDPCs was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The mineralization potential of HDPCs was investigated with the relative gene expression of alkaline phosphatase (ALP), collagen type I (ColI), osteocalcin (OCN), and runt-related transcription factor 2 (Runx2) using real-time reverse transcription polymerase chain reaction (RT-PCR). For investigating the physical properties, setting time and solubility were tested. Surface profiles of material were analyzed by a non-contact surface profiler and a scanning electron microscope (SEM). Results: MTA-5% CaF2 mixtures increased the proliferation and the mineralization-related gene expression of HDPCs to a greater degree than pure MTA. The addition of CaF2 to MTA delayed the setting, but the difference was only significant in the MTA-10% CaF2. Solubility and surface roughness was not altered. Conclusion: The addition of more than 5% CaF2 can be considered to increase the regeneration potential of pulp cells without adverse effects on physical property.http://www.sciencedirect.com/science/article/pii/S1991790221000994BiocompatibilityCalcium fluorideMineralizationMTAPhysical property |
| spellingShingle | Miyoung Lim Minju Song Chan-Ui Hong Yong-bum Cho The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study Journal of Dental Sciences Biocompatibility Calcium fluoride Mineralization MTA Physical property |
| title | The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study |
| title_full | The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study |
| title_fullStr | The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study |
| title_full_unstemmed | The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study |
| title_short | The biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride–An in vitro study |
| title_sort | biocompatibility and mineralization potential of mineral trioxide aggregate containing calcium fluoride an in vitro study |
| topic | Biocompatibility Calcium fluoride Mineralization MTA Physical property |
| url | http://www.sciencedirect.com/science/article/pii/S1991790221000994 |
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