Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions
The zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, <i>Lactobacillus paracasei</i>, <i>Lactococcus lactis,</i> and yeast <i>Saccharomyces cerevisiae,</i> isolated from food products, were examined. Moreover...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/8/463 |
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| author | Agnieszka Rogowska Paweł Pomastowski Justyna Walczak Viorica Railean-Plugaru Joanna Rudnicka Bogusław Buszewski |
| author_facet | Agnieszka Rogowska Paweł Pomastowski Justyna Walczak Viorica Railean-Plugaru Joanna Rudnicka Bogusław Buszewski |
| author_sort | Agnieszka Rogowska |
| collection | DOAJ |
| description | The zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, <i>Lactobacillus paracasei</i>, <i>Lactococcus lactis,</i> and yeast <i>Saccharomyces cerevisiae,</i> isolated from food products, were examined. Moreover, the influence of cellular stress (induced by silver nanoparticles) and lyophilization on the effectiveness of tested microorganisms was also investigated. The concentration of zearalenone after a certain time of incubation with microorganisms was determined using high-performance liquid chromatography. The maximum sorption effectiveness for <i>L. paracasei</i>, <i>L. lactis,</i> and <i>S. cerevisiae</i> cultured in non-stress conditions was 53.3, 41.0, and 36.5%, respectively. At the same time for the same microorganisms cultured at cellular stress conditions, the maximum sorption effectiveness was improved to 55.3, 47.4, and 57.0%, respectively. Also, the effect of culture conditions on the morphology of the cells and its metabolism was examined using microscopic technique and matrix-assisted laser desorption ionization-time of flight mass spectrometry, respectively. |
| first_indexed | 2024-04-14T00:29:17Z |
| format | Article |
| id | doaj.art-78c40ca121ff43c9a452f7f554abe6c9 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T00:29:17Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-78c40ca121ff43c9a452f7f554abe6c92022-12-22T02:22:35ZengMDPI AGToxins2072-66512019-08-0111846310.3390/toxins11080463toxins11080463Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress ConditionsAgnieszka Rogowska0Paweł Pomastowski1Justyna Walczak2Viorica Railean-Plugaru3Joanna Rudnicka4Bogusław Buszewski5Centre for Modern Interdisciplinary Technologies Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Torun, PolandThe zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, <i>Lactobacillus paracasei</i>, <i>Lactococcus lactis,</i> and yeast <i>Saccharomyces cerevisiae,</i> isolated from food products, were examined. Moreover, the influence of cellular stress (induced by silver nanoparticles) and lyophilization on the effectiveness of tested microorganisms was also investigated. The concentration of zearalenone after a certain time of incubation with microorganisms was determined using high-performance liquid chromatography. The maximum sorption effectiveness for <i>L. paracasei</i>, <i>L. lactis,</i> and <i>S. cerevisiae</i> cultured in non-stress conditions was 53.3, 41.0, and 36.5%, respectively. At the same time for the same microorganisms cultured at cellular stress conditions, the maximum sorption effectiveness was improved to 55.3, 47.4, and 57.0%, respectively. Also, the effect of culture conditions on the morphology of the cells and its metabolism was examined using microscopic technique and matrix-assisted laser desorption ionization-time of flight mass spectrometry, respectively.https://www.mdpi.com/2072-6651/11/8/463mycotoxinsprobiotic microorganismssilver nanoparticlesMALDI-TOF MSmetabolism |
| spellingShingle | Agnieszka Rogowska Paweł Pomastowski Justyna Walczak Viorica Railean-Plugaru Joanna Rudnicka Bogusław Buszewski Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions Toxins mycotoxins probiotic microorganisms silver nanoparticles MALDI-TOF MS metabolism |
| title | Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions |
| title_full | Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions |
| title_fullStr | Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions |
| title_full_unstemmed | Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions |
| title_short | Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions |
| title_sort | investigation of zearalenone adsorption and biotransformation by microorganisms cultured under cellular stress conditions |
| topic | mycotoxins probiotic microorganisms silver nanoparticles MALDI-TOF MS metabolism |
| url | https://www.mdpi.com/2072-6651/11/8/463 |
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