Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage
Background: Resistance to contemporary broad-spectrum b-lactam antibiotics mediated by extended-spectrum b-lactamases (ESBLs) is increasing worldwide. Klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common ESBL produ...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2012-04-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20509.pdf&manuscript_id=20509 |
| _version_ | 1818608348259418112 |
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| author | N MansourSamaei F Eftekhar M Golalipoor M Rastegar |
| author_facet | N MansourSamaei F Eftekhar M Golalipoor M Rastegar |
| author_sort | N MansourSamaei |
| collection | DOAJ |
| description | Background: Resistance to contemporary broad-spectrum b-lactam antibiotics mediated by extended-spectrum b-lactamases (ESBLs) is increasing worldwide. Klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common ESBL producing organism. We examined ESBL production in urinary isolates of K. pneumoniae in relation to the presence of blaSHV, blaTEM and blaCTX-M genes.Methods: Antibiotic resistance of 51 clinical isolates of K. pneumoniae was determined to amoxicillin, amikacin, ceftazidime, cefotaxime, cefteriaxon, ceftizoxime, gentamicin, ciprofloxacin and nitrofurantoin by disc diffusion. Minimum inhibitory concentrations were also measured for ceftazidime, cefotaxime, cefteriaxon, ceftizoxime and ciprofloxacin. ESBL production was detected by the double disc synergy test and finally, presence of the blaSHV, blaTEM and blaCTX-M genes were shown using specific primers and PCR.Results: Disc diffusion results showed that 96.08 % of the isolates were resistant to amoxicillin followed by 78.43 % resistance to nitrofurantoin, 49.02 % to amikacin and ceftazidime, 41.17 % to ceftriaxone, 37.25% resistance to cefotaxime and ceftizoxime, and 29.42 % to gentamicin and ciprofloxacin. Both resistant and intermediately resistant organisms were resistant in MIC determinations. Twenty two isolates (43.14%) carried blaSHV, 18 (35.29%) had blaTEM and 16 (31.37%) harbored blaCTX-M genes. ESBL production was present in 14 isolates (27.45 %) of which, 3 did not harbor any of the 3 genes. Among the non- ESBL producers, 9 lacked all 3 genes and 2 carried them all.Conclusion: No relation was found between gene presence and ESBL expression. |
| first_indexed | 2024-12-16T14:41:13Z |
| format | Article |
| id | doaj.art-78c46a33b97e4d9baed930384d4f4238 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-16T14:41:13Z |
| publishDate | 2012-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-78c46a33b97e4d9baed930384d4f42382022-12-21T22:27:55ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852012-04-01413127132Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene CarriageN MansourSamaeiF EftekharM GolalipoorM RastegarBackground: Resistance to contemporary broad-spectrum b-lactam antibiotics mediated by extended-spectrum b-lactamases (ESBLs) is increasing worldwide. Klebsiella pneumoniae, an important cause of nosocomial and community acquired urinary tract infections has rapidly become the most common ESBL producing organism. We examined ESBL production in urinary isolates of K. pneumoniae in relation to the presence of blaSHV, blaTEM and blaCTX-M genes.Methods: Antibiotic resistance of 51 clinical isolates of K. pneumoniae was determined to amoxicillin, amikacin, ceftazidime, cefotaxime, cefteriaxon, ceftizoxime, gentamicin, ciprofloxacin and nitrofurantoin by disc diffusion. Minimum inhibitory concentrations were also measured for ceftazidime, cefotaxime, cefteriaxon, ceftizoxime and ciprofloxacin. ESBL production was detected by the double disc synergy test and finally, presence of the blaSHV, blaTEM and blaCTX-M genes were shown using specific primers and PCR.Results: Disc diffusion results showed that 96.08 % of the isolates were resistant to amoxicillin followed by 78.43 % resistance to nitrofurantoin, 49.02 % to amikacin and ceftazidime, 41.17 % to ceftriaxone, 37.25% resistance to cefotaxime and ceftizoxime, and 29.42 % to gentamicin and ciprofloxacin. Both resistant and intermediately resistant organisms were resistant in MIC determinations. Twenty two isolates (43.14%) carried blaSHV, 18 (35.29%) had blaTEM and 16 (31.37%) harbored blaCTX-M genes. ESBL production was present in 14 isolates (27.45 %) of which, 3 did not harbor any of the 3 genes. Among the non- ESBL producers, 9 lacked all 3 genes and 2 carried them all.Conclusion: No relation was found between gene presence and ESBL expression.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20509.pdf&manuscript_id=20509Klebsiella PneumoniaeUrinary InfectionExtended Spectrum -lactamasesBlaSHVBlaTEMBlaCTX-M |
| spellingShingle | N MansourSamaei F Eftekhar M Golalipoor M Rastegar Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage Iranian Journal of Public Health Klebsiella Pneumoniae Urinary Infection Extended Spectrum -lactamases BlaSHV BlaTEM BlaCTX-M |
| title | Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage |
| title_full | Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage |
| title_fullStr | Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage |
| title_full_unstemmed | Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage |
| title_short | Detection of Extended Spectrum Β-Lactamases in Urinary Isolates of Klebsiella Pneumoniae in Relation to BlaSHV, BlaTEM and BlaCTX-M Gene Carriage |
| title_sort | detection of extended spectrum β lactamases in urinary isolates of klebsiella pneumoniae in relation to blashv blatem and blactx m gene carriage |
| topic | Klebsiella Pneumoniae Urinary Infection Extended Spectrum -lactamases BlaSHV BlaTEM BlaCTX-M |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/20509.pdf&manuscript_id=20509 |
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