Nicotiana glauca whole-genome investigation for cT-DNA study
Abstract Objective Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description We report a high-depth sequencing...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3127-x |
| _version_ | 1818107808895205376 |
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| author | Galina Khafizova Pavel Dobrynin Dmitrii Polev Tatiana Matveeva |
| author_facet | Galina Khafizova Pavel Dobrynin Dmitrii Polev Tatiana Matveeva |
| author_sort | Galina Khafizova |
| collection | DOAJ |
| description | Abstract Objective Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields. |
| first_indexed | 2024-12-11T02:05:21Z |
| format | Article |
| id | doaj.art-78c4a614205a4a559da8049049e69a85 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-11T02:05:21Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-78c4a614205a4a559da8049049e69a852022-12-22T01:24:24ZengBMCBMC Research Notes1756-05002018-01-011111310.1186/s13104-018-3127-xNicotiana glauca whole-genome investigation for cT-DNA studyGalina Khafizova0Pavel Dobrynin1Dmitrii Polev2Tatiana Matveeva3Department of Genetics and Biotechnology, Saint Petersburg State UniversityTheodosius Dobzhansky Center for Genome Bioinformatics, Saint Petersburg State UniversityResearch Park, Saint Petersburg State UniversityDepartment of Genetics and Biotechnology, Saint Petersburg State UniversityAbstract Objective Nicotiana glauca (tree tobacco) is a naturally transgenic plant, containing sequences acquired from Agrobacterium rhizogenes by horizontal gene transfer. Besides, N. glauca contains a wide profile of alkaloids of medical interest. Data description We report a high-depth sequencing and de novo assembly of N. glauca full genome and analysis of genome elements with bacterial origin. The draft genome assembly is 3.2 Gb, with N50 size of 31.1 kbp. Comparative analysis confirmed the presence of single, previously described gT insertion. No evidence was acquired to support idea of multiple T-DNA insertions in the N. glauca genome. Our data is the first comprehensive de novo assembly of tree tobacco and provide valuable information for researches in pharmacological and in phylogenetic fields.http://link.springer.com/article/10.1186/s13104-018-3127-xNicotiana glaucaCellular T-DNAWhole genome sequencingGenome assembly |
| spellingShingle | Galina Khafizova Pavel Dobrynin Dmitrii Polev Tatiana Matveeva Nicotiana glauca whole-genome investigation for cT-DNA study BMC Research Notes Nicotiana glauca Cellular T-DNA Whole genome sequencing Genome assembly |
| title | Nicotiana glauca whole-genome investigation for cT-DNA study |
| title_full | Nicotiana glauca whole-genome investigation for cT-DNA study |
| title_fullStr | Nicotiana glauca whole-genome investigation for cT-DNA study |
| title_full_unstemmed | Nicotiana glauca whole-genome investigation for cT-DNA study |
| title_short | Nicotiana glauca whole-genome investigation for cT-DNA study |
| title_sort | nicotiana glauca whole genome investigation for ct dna study |
| topic | Nicotiana glauca Cellular T-DNA Whole genome sequencing Genome assembly |
| url | http://link.springer.com/article/10.1186/s13104-018-3127-x |
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