Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial
Background Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal...
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| Format: | Article |
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Royan Institute (ACECR), Tehran
2020-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45565_fbfe6a9f727ec0e5d9c970e89b9c1eae.pdf |
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| author | Abdul Razzaq S. Alsalman Lamia A. Almashhedy Mahmoud H. Hadwan Abdulsamie H. Alta'ee |
| author_facet | Abdul Razzaq S. Alsalman Lamia A. Almashhedy Mahmoud H. Hadwan Abdulsamie H. Alta'ee |
| author_sort | Abdul Razzaq S. Alsalman |
| collection | DOAJ |
| description | Background Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal fluid, in parallel with the UA level and urate pathway enzymes in the semen of patients with asthenozoospermia. Materials and Methods The study was designed as a randomized controlled trial of 60 asthenozoospermic subfertile men. The current study, which was conducted during one year, involved 60 fertile and 60 asthenozoospermic subfertile men belonging to Hilla City, Iraq. Semen samples were obtained from the participants before and after treatment with zinc supplements. The levels of UA, xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NU) activities were determined in spermatozoa and seminal plasma of both groups. Results UA levels (P=0.034) and 5'-NU activity (P=0.046) were significantly lower but ADA (P=0.05) and XO (P=0.015) activities were significantly higher in infertile men than in healthy men. Treatment with zinc sulfate induced an increase in UA (P=0.001) level and 5'-NU activity (P=0.001), but a decrease in ADA (P=0.016) and XO (P=0.05) activities. Conclusion Zinc supplementation restores UA levels and the activities of enzymes involved in the urate pathway (XO and ADA) in the seminal plasma and spermatozoa of patients with asthenozoospermia, to reference values. Sup- plementation of Zn compounds enhances the qualitative and quantitative properties of semen (Registration number: NCT03361618). |
| first_indexed | 2024-12-13T05:18:19Z |
| format | Article |
| id | doaj.art-78cd53b789ea424aaaa9c7ef338d47ab |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-13T05:18:19Z |
| publishDate | 2020-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-78cd53b789ea424aaaa9c7ef338d47ab2022-12-21T23:58:23ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782020-01-0113431532310.22074/ijfs.2020.576045565Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled TrialAbdul Razzaq S. Alsalman0Lamia A. Almashhedy1Mahmoud H. Hadwan2Abdulsamie H. Alta'ee3College of Medicine, University of Babylon, Hilla, IraqChemistry Department, College of Science, University of Babylon, Hilla, IraqChemistry Department, College of Science, University of Babylon, Hilla, IraqCollege of Medicine, University of Babylon, Hilla, Iraq;College of Pharmacy, University of Babylon, Hilla, IraqBackground Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal fluid, in parallel with the UA level and urate pathway enzymes in the semen of patients with asthenozoospermia. Materials and Methods The study was designed as a randomized controlled trial of 60 asthenozoospermic subfertile men. The current study, which was conducted during one year, involved 60 fertile and 60 asthenozoospermic subfertile men belonging to Hilla City, Iraq. Semen samples were obtained from the participants before and after treatment with zinc supplements. The levels of UA, xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NU) activities were determined in spermatozoa and seminal plasma of both groups. Results UA levels (P=0.034) and 5'-NU activity (P=0.046) were significantly lower but ADA (P=0.05) and XO (P=0.015) activities were significantly higher in infertile men than in healthy men. Treatment with zinc sulfate induced an increase in UA (P=0.001) level and 5'-NU activity (P=0.001), but a decrease in ADA (P=0.016) and XO (P=0.05) activities. Conclusion Zinc supplementation restores UA levels and the activities of enzymes involved in the urate pathway (XO and ADA) in the seminal plasma and spermatozoa of patients with asthenozoospermia, to reference values. Sup- plementation of Zn compounds enhances the qualitative and quantitative properties of semen (Registration number: NCT03361618).http://www.ijfs.ir/article_45565_fbfe6a9f727ec0e5d9c970e89b9c1eae.pdfadenosine deaminase5'nucleotidaseuric acidxanthine oxidasezinc supplementation |
| spellingShingle | Abdul Razzaq S. Alsalman Lamia A. Almashhedy Mahmoud H. Hadwan Abdulsamie H. Alta'ee Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial International Journal of Fertility and Sterility adenosine deaminase 5' nucleotidase uric acid xanthine oxidase zinc supplementation |
| title | Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial |
| title_full | Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial |
| title_fullStr | Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial |
| title_full_unstemmed | Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial |
| title_short | Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial |
| title_sort | effect of zinc supplementation on urate pathway enzymes in spermatozoa and seminal plasma of iraqi asthenozoospermic patients a randomized controlled trial |
| topic | adenosine deaminase 5' nucleotidase uric acid xanthine oxidase zinc supplementation |
| url | http://www.ijfs.ir/article_45565_fbfe6a9f727ec0e5d9c970e89b9c1eae.pdf |
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