Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
<p>Abstract</p> <p>Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results dem...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/42/1/8 |
| _version_ | 1811244513004355584 |
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| author | Løvoll Marie Austbø Lars Jørgensen Jorunn B Rimstad Espen Frost Petter |
| author_facet | Løvoll Marie Austbø Lars Jørgensen Jorunn B Rimstad Espen Frost Petter |
| author_sort | Løvoll Marie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon pancreas disease virus (SPDV). Transcript levels 0 to 6 weeks after challenge revealed statistically significant changes between time-points that corresponded with a peak in viral load 3 weeks after challenge. The results emphasize the need for thorough method validation prior to transcriptional studies during viral infections.</p> |
| first_indexed | 2024-04-12T14:26:23Z |
| format | Article |
| id | doaj.art-78d326449d25490b8c7be4faf1a7f15e |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-04-12T14:26:23Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-78d326449d25490b8c7be4faf1a7f15e2022-12-22T03:29:25ZengBMCVeterinary Research0928-42491297-97162011-01-01421810.1186/1297-9716-42-8Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infectionLøvoll MarieAustbø LarsJørgensen Jorunn BRimstad EspenFrost Petter<p>Abstract</p> <p>Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon pancreas disease virus (SPDV). Transcript levels 0 to 6 weeks after challenge revealed statistically significant changes between time-points that corresponded with a peak in viral load 3 weeks after challenge. The results emphasize the need for thorough method validation prior to transcriptional studies during viral infections.</p>http://www.veterinaryresearch.org/content/42/1/8 |
| spellingShingle | Løvoll Marie Austbø Lars Jørgensen Jorunn B Rimstad Espen Frost Petter Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection Veterinary Research |
| title | Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection |
| title_full | Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection |
| title_fullStr | Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection |
| title_full_unstemmed | Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection |
| title_short | Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection |
| title_sort | transcription of reference genes used for quantitative rt pcr in atlantic salmon is affected by viral infection |
| url | http://www.veterinaryresearch.org/content/42/1/8 |
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