Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density
Multiple methodologies have been reported to facilitate skin-derived precursor (SKP) growth, but the impact of plating density on SKP growth has not been studied. To determine the optimal plating density, we used six plating densities and two types of flasks for mouse SKP (mSKP) culture. On the 14th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-12-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2021-0128 |
| _version_ | 1811203773528276992 |
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| author | Li Yiming Xiong Lidan Tang Jie Dai Ru Li Shiyi Li Li |
| author_facet | Li Yiming Xiong Lidan Tang Jie Dai Ru Li Shiyi Li Li |
| author_sort | Li Yiming |
| collection | DOAJ |
| description | Multiple methodologies have been reported to facilitate skin-derived precursor (SKP) growth, but the impact of plating density on SKP growth has not been studied. To determine the optimal plating density, we used six plating densities and two types of flasks for mouse SKP (mSKP) culture. On the 14th day, the number, diameter, and viability of mSKP spheres were compared by morphological assessment and cell counting kit 8, and we found the optimal plating density was 2.5 × 105–5 × 105 cells/mL. In addition, we investigated the correlation between the SKP spheres and the adherent cell colonies in the serum-free culture system. We treated the adherent cell colonies with two culture conditions and characterized the cells generated from two conditions by immunocytochemistry and induced differentiation, respectively. The results elucidated that the adherent cell colonies differentiated into either mSKPs or dermal mesenchymal stem cells under appropriate culture conditions. In conclusion, mSKP spheres differentiated from the adherent cell colonies. The optimal plating density significantly promoted and advanced the proliferation of adherent cell colonies, which optimized mSKP growth and yield. The adherent cell colonies possessed the capacity of differentiating into different types of cells under appropriate culture conditions. |
| first_indexed | 2024-04-12T03:00:47Z |
| format | Article |
| id | doaj.art-78d438c08b8241fab9f773346b3d8f21 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-04-12T03:00:47Z |
| publishDate | 2021-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-78d438c08b8241fab9f773346b3d8f212022-12-22T03:50:40ZengDe GruyterOpen Life Sciences2391-54122021-12-011611293130210.1515/biol-2021-0128Facilitation of mouse skin-derived precursor growth and yield by optimizing plating densityLi Yiming0Xiong Lidan1Tang Jie2Dai Ru3Li Shiyi4Li Li5 Department of Dermatology and Venerology, West China Hospital, Sichuan University, 37 Guoxue Alley, Chengdu, Sichuan Province 610041, China Department of Dermatology and Venerology, West China Hospital, Sichuan University, 37 Guoxue Alley, Chengdu, Sichuan Province 610041, China Department of Dermatology and Venerology, West China Hospital, Sichuan University, 37 Guoxue Alley, Chengdu, Sichuan Province 610041, China Department of Dermatology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310009, ChinaLaboratory of Ethnopharmacology, West China Hospital, Sichuan University, Gaopeng Avenue, Gaoxin District, Chengdu, Sichuan Province 610041, China Department of Dermatology and Venerology, West China Hospital, Sichuan University, 37 Guoxue Alley, Chengdu, Sichuan Province 610041, ChinaMultiple methodologies have been reported to facilitate skin-derived precursor (SKP) growth, but the impact of plating density on SKP growth has not been studied. To determine the optimal plating density, we used six plating densities and two types of flasks for mouse SKP (mSKP) culture. On the 14th day, the number, diameter, and viability of mSKP spheres were compared by morphological assessment and cell counting kit 8, and we found the optimal plating density was 2.5 × 105–5 × 105 cells/mL. In addition, we investigated the correlation between the SKP spheres and the adherent cell colonies in the serum-free culture system. We treated the adherent cell colonies with two culture conditions and characterized the cells generated from two conditions by immunocytochemistry and induced differentiation, respectively. The results elucidated that the adherent cell colonies differentiated into either mSKPs or dermal mesenchymal stem cells under appropriate culture conditions. In conclusion, mSKP spheres differentiated from the adherent cell colonies. The optimal plating density significantly promoted and advanced the proliferation of adherent cell colonies, which optimized mSKP growth and yield. The adherent cell colonies possessed the capacity of differentiating into different types of cells under appropriate culture conditions.https://doi.org/10.1515/biol-2021-0128skin-derived precursorsdermal mesenchymal stem cellsdensitycell cultureinduced differentiation |
| spellingShingle | Li Yiming Xiong Lidan Tang Jie Dai Ru Li Shiyi Li Li Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density Open Life Sciences skin-derived precursors dermal mesenchymal stem cells density cell culture induced differentiation |
| title | Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density |
| title_full | Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density |
| title_fullStr | Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density |
| title_full_unstemmed | Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density |
| title_short | Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density |
| title_sort | facilitation of mouse skin derived precursor growth and yield by optimizing plating density |
| topic | skin-derived precursors dermal mesenchymal stem cells density cell culture induced differentiation |
| url | https://doi.org/10.1515/biol-2021-0128 |
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