Porphyrin-modified antimicrobial peptide indicators for detection of bacteria
This study demonstrates the potential of porphyrin modified antimicrobial peptides for indication of bacterial targets on the basis of changes in the spectrophotometric characteristics of the construct. Detection is a result of changes in the structure of the antimicrobial peptide upon target bindin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-05-01
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| Series: | Sensing and Bio-Sensing Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180416300174 |
| _version_ | 1818130252415631360 |
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| author | Brandy J. Johnson Chris R. Taitt Apre Gleaves Stella H. North Anthony P. Malanoski Iwona A. Leska Edikan Archibong Stormie M. Monk |
| author_facet | Brandy J. Johnson Chris R. Taitt Apre Gleaves Stella H. North Anthony P. Malanoski Iwona A. Leska Edikan Archibong Stormie M. Monk |
| author_sort | Brandy J. Johnson |
| collection | DOAJ |
| description | This study demonstrates the potential of porphyrin modified antimicrobial peptides for indication of bacterial targets on the basis of changes in the spectrophotometric characteristics of the construct. Detection is a result of changes in the structure of the antimicrobial peptide upon target binding. Those constructs comprised of peptides that offer little or no change in conformation upon interaction with bacterial cells demonstrated negligible changes in absorbance and fluorescence when challenged using Escherichia coli or Bacillus cereus. CD analysis confirms the presence/absence of conformational changes in the porphyrin-peptide constructs. Differing spectrophotometric responses were observed for constructs utilizing different peptides. The incorporation of metals into the porphyrin component of the constructs was shown to alter their spectrophotometric characteristics as well as the resulting absorbance and fluorescence changes noted upon interaction with a target. The described constructs offer the potential to enable a new type of biosensing approach in which the porphyrin-peptide indicators offer both target recognition and optical transduction, requiring no additional reagents. Keywords: Antimicrobial peptide, Porphyrin, Bacterial detection, Absorbance, Fluorescence |
| first_indexed | 2024-12-11T08:02:05Z |
| format | Article |
| id | doaj.art-78d954552d67402ca67cf19571b437a8 |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-12-11T08:02:05Z |
| publishDate | 2016-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-78d954552d67402ca67cf19571b437a82022-12-22T01:15:05ZengElsevierSensing and Bio-Sensing Research2214-18042016-05-01817Porphyrin-modified antimicrobial peptide indicators for detection of bacteriaBrandy J. Johnson0Chris R. Taitt1Apre Gleaves2Stella H. North3Anthony P. Malanoski4Iwona A. Leska5Edikan Archibong6Stormie M. Monk7Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA; Corresponding author.Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USAChemistry Department, Howard University, Washington, DC 20059, USACenter for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USACenter for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USANOVA Research Incorporated, Alexandria, VA 22308, USADepartment of Chemical and Biomedical Engineering, University of South Florida, Tampa, FL 33620, USAFayetteville State University, Fayetteville, NC 28301, USAThis study demonstrates the potential of porphyrin modified antimicrobial peptides for indication of bacterial targets on the basis of changes in the spectrophotometric characteristics of the construct. Detection is a result of changes in the structure of the antimicrobial peptide upon target binding. Those constructs comprised of peptides that offer little or no change in conformation upon interaction with bacterial cells demonstrated negligible changes in absorbance and fluorescence when challenged using Escherichia coli or Bacillus cereus. CD analysis confirms the presence/absence of conformational changes in the porphyrin-peptide constructs. Differing spectrophotometric responses were observed for constructs utilizing different peptides. The incorporation of metals into the porphyrin component of the constructs was shown to alter their spectrophotometric characteristics as well as the resulting absorbance and fluorescence changes noted upon interaction with a target. The described constructs offer the potential to enable a new type of biosensing approach in which the porphyrin-peptide indicators offer both target recognition and optical transduction, requiring no additional reagents. Keywords: Antimicrobial peptide, Porphyrin, Bacterial detection, Absorbance, Fluorescencehttp://www.sciencedirect.com/science/article/pii/S2214180416300174 |
| spellingShingle | Brandy J. Johnson Chris R. Taitt Apre Gleaves Stella H. North Anthony P. Malanoski Iwona A. Leska Edikan Archibong Stormie M. Monk Porphyrin-modified antimicrobial peptide indicators for detection of bacteria Sensing and Bio-Sensing Research |
| title | Porphyrin-modified antimicrobial peptide indicators for detection of bacteria |
| title_full | Porphyrin-modified antimicrobial peptide indicators for detection of bacteria |
| title_fullStr | Porphyrin-modified antimicrobial peptide indicators for detection of bacteria |
| title_full_unstemmed | Porphyrin-modified antimicrobial peptide indicators for detection of bacteria |
| title_short | Porphyrin-modified antimicrobial peptide indicators for detection of bacteria |
| title_sort | porphyrin modified antimicrobial peptide indicators for detection of bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S2214180416300174 |
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