β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients
Aim. To develop a test system measuring β1-adrenoreceptor antibody (anti- β1-AR) level. To study the effects of autoantibody removal on left ventricular (LV) contractility. Material and methods. Peptides, according to second human extracellular β1-AR loop fragments (197-222 amino acid fragments), we...
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| Format: | Article |
| Language: | Russian |
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«SILICEA-POLIGRAF» LLC
2007-06-01
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| Series: | Кардиоваскулярная терапия и профилактика |
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| Online Access: | https://cardiovascular.elpub.ru/jour/article/view/1239 |
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| author | E. A. Tabakyan A. G. Tonevitsky D. M. Ataullakhanova A. Yu. Zaruba V. V. Kukharchuk |
| author_facet | E. A. Tabakyan A. G. Tonevitsky D. M. Ataullakhanova A. Yu. Zaruba V. V. Kukharchuk |
| author_sort | E. A. Tabakyan |
| collection | DOAJ |
| description | Aim. To develop a test system measuring β1-adrenoreceptor antibody (anti- β1-AR) level. To study the effects of autoantibody removal on left ventricular (LV) contractility. Material and methods. Peptides, according to second human extracellular β1-AR loop fragments (197-222 amino acid fragments), were synthesized by modified hard-phase method and then lyophilized. Molecular mass control was performed by laser desorption mass spectrometry. In total, 47 patients were examined, with the aim of anti- β1-AR detection. Dilated cardiomyopathy (DCMP) was diagnosed in 22 patients, ischemic CMP – in 8, post-infarction cardiosclerosis – in 6, myocarditis – in 3, alcohol CMP – in 1, and post-transplantation CMP – in 7 participants. Anti- β1-AR were removed in 4 DCMP patients, by plasmapheresis (PF; n=3) or immunoadsorption (IA; n=1). Results. A new immune-enzyme test system for autoantibody detection has been developed, using the second extracellular β1-AR loop 26 amino acid peptide as the antigen. Anti- β1-AR removal by IA or PF methods resulted in improved LV contractility among DCMP patients. Conclusion. It is important to determine whether LV contractility improvement is explained by anti-β1-AR removal exclusively. More advanced methods for anti- β1-AR and other anti-myocardial antibody detection should be developed, and auto-antibodies’ role in impaired myocardial contractility should be studied. |
| first_indexed | 2024-04-10T03:40:35Z |
| format | Article |
| id | doaj.art-78da11982ec04537b89009ff65efaede |
| institution | Directory Open Access Journal |
| issn | 1728-8800 2619-0125 |
| language | Russian |
| last_indexed | 2024-04-10T03:40:35Z |
| publishDate | 2007-06-01 |
| publisher | «SILICEA-POLIGRAF» LLC |
| record_format | Article |
| series | Кардиоваскулярная терапия и профилактика |
| spelling | doaj.art-78da11982ec04537b89009ff65efaede2023-03-13T07:23:13Zrus«SILICEA-POLIGRAF» LLCКардиоваскулярная терапия и профилактика1728-88002619-01252007-06-01632532950β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patientsE. A. Tabakyan0A. G. Tonevitsky1D. M. Ataullakhanova2A. Yu. Zaruba3V. V. Kukharchuk4НИИ клинической кардиологии им. А.Л.Мясникова Российского кардиологического научно)производственного комплекса Росздрава, МоскваНИИ трансплантологии и искусственных органов РосздраваНИИ клинической кардиологии им. А.Л.Мясникова Российского кардиологического научно)производственного комплекса Росздрава, МоскваНИИ клинической кардиологии им. А.Л.Мясникова Российского кардиологического научно)производственного комплекса Росздрава, МоскваНИИ клинической кардиологии им. А.Л.Мясникова Российского кардиологического научно)производственного комплекса Росздрава, МоскваAim. To develop a test system measuring β1-adrenoreceptor antibody (anti- β1-AR) level. To study the effects of autoantibody removal on left ventricular (LV) contractility. Material and methods. Peptides, according to second human extracellular β1-AR loop fragments (197-222 amino acid fragments), were synthesized by modified hard-phase method and then lyophilized. Molecular mass control was performed by laser desorption mass spectrometry. In total, 47 patients were examined, with the aim of anti- β1-AR detection. Dilated cardiomyopathy (DCMP) was diagnosed in 22 patients, ischemic CMP – in 8, post-infarction cardiosclerosis – in 6, myocarditis – in 3, alcohol CMP – in 1, and post-transplantation CMP – in 7 participants. Anti- β1-AR were removed in 4 DCMP patients, by plasmapheresis (PF; n=3) or immunoadsorption (IA; n=1). Results. A new immune-enzyme test system for autoantibody detection has been developed, using the second extracellular β1-AR loop 26 amino acid peptide as the antigen. Anti- β1-AR removal by IA or PF methods resulted in improved LV contractility among DCMP patients. Conclusion. It is important to determine whether LV contractility improvement is explained by anti-β1-AR removal exclusively. More advanced methods for anti- β1-AR and other anti-myocardial antibody detection should be developed, and auto-antibodies’ role in impaired myocardial contractility should be studied.https://cardiovascular.elpub.ru/jour/article/view/1239аутоантитела против β1-адренорецептораиммуноадсорбцияплазмаферезсократимость миокарда левого желудочка |
| spellingShingle | E. A. Tabakyan A. G. Tonevitsky D. M. Ataullakhanova A. Yu. Zaruba V. V. Kukharchuk β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients Кардиоваскулярная терапия и профилактика аутоантитела против β1-адренорецептора иммуноадсорбция плазмаферез сократимость миокарда левого желудочка |
| title | β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients |
| title_full | β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients |
| title_fullStr | β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients |
| title_full_unstemmed | β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients |
| title_short | β1-adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients |
| title_sort | β1 adrenoreceptor antibody measurement and antibody removal effects on left ventricular contractility in dilated cardiomyopathy patients |
| topic | аутоантитела против β1-адренорецептора иммуноадсорбция плазмаферез сократимость миокарда левого желудочка |
| url | https://cardiovascular.elpub.ru/jour/article/view/1239 |
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