NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis
Excess ammonia (NH3) in the circulation of dairy animals can reduce animal health and the quality of products for human consumption. To develop effective prevention and treatment methods, it is essential to examine the molecular mechanisms through which excess NH3 may affect the mammary gland. The p...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921638121 |
| _version_ | 1818157807488204800 |
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| author | Zhi CHEN Yu-sheng LIANG Wei-cheng ZONG Jia-he GUO Jing-peng ZHOU Yong-jiang MAO De-jun JI Pei-xin JIAO LOOR Juan J Zhang-ping YANG |
| author_facet | Zhi CHEN Yu-sheng LIANG Wei-cheng ZONG Jia-he GUO Jing-peng ZHOU Yong-jiang MAO De-jun JI Pei-xin JIAO LOOR Juan J Zhang-ping YANG |
| author_sort | Zhi CHEN |
| collection | DOAJ |
| description | Excess ammonia (NH3) in the circulation of dairy animals can reduce animal health and the quality of products for human consumption. To develop effective prevention and treatment methods, it is essential to examine the molecular mechanisms through which excess NH3 may affect the mammary gland. The present study used bovine mammary epithelial cells (BMECs) to evaluate the effects of exogenous NH4Cl on the abundance of circular RNAs (circRNAs) using high-throughput sequencing. Among the identified circRNAs, circ02771 was the most significantly upregulated by exogenous NH4Cl (P<0.05), with a fold change of 4.12. The results of the apoptosis and proliferation assays, transmission electron microscopy, H&E staining, and immunohistochemistry revealed that circ02771 increased apoptosis and inflammation. A double luciferase reporter assay revealed that circ02771 targeted miR-194b, and the overexpression of circ02771 (pcDNA-circ02771) reduced (P<0.05) the expression of miR-194b and led to apoptosis and inflammation. Circ02771 also enhanced the expression of transforming growth factor beta-induced factor homeobox 1 (TGIF1), which is a target gene of miR-194b. Overall, this study suggests that the circ02771/miR-194b/TGIF1 axis plays a role in mediating the effects of NH4Cl on BMECs. Therefore, this axis provides a novel target to help control hazards within the mammary gland from high circulating NH4Cl levels. |
| first_indexed | 2024-12-11T15:20:04Z |
| format | Article |
| id | doaj.art-78dbc32bc81b4c248ac3f4a3103d1deb |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-11T15:20:04Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-78dbc32bc81b4c248ac3f4a3103d1deb2022-12-22T01:00:24ZengElsevierJournal of Integrative Agriculture2095-31192022-04-0121411611176NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axisZhi CHEN0Yu-sheng LIANG1Wei-cheng ZONG2Jia-he GUO3Jing-peng ZHOU4Yong-jiang MAO5De-jun JI6Pei-xin JIAO7LOOR Juan J8Zhang-ping YANG9College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.ChinaMammalian Nutrition Physiology Genomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USACollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.ChinaMammalian Nutrition Physiology Genomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USACollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence YANG Zhang-ping, Tel: +86-514-87979269Excess ammonia (NH3) in the circulation of dairy animals can reduce animal health and the quality of products for human consumption. To develop effective prevention and treatment methods, it is essential to examine the molecular mechanisms through which excess NH3 may affect the mammary gland. The present study used bovine mammary epithelial cells (BMECs) to evaluate the effects of exogenous NH4Cl on the abundance of circular RNAs (circRNAs) using high-throughput sequencing. Among the identified circRNAs, circ02771 was the most significantly upregulated by exogenous NH4Cl (P<0.05), with a fold change of 4.12. The results of the apoptosis and proliferation assays, transmission electron microscopy, H&E staining, and immunohistochemistry revealed that circ02771 increased apoptosis and inflammation. A double luciferase reporter assay revealed that circ02771 targeted miR-194b, and the overexpression of circ02771 (pcDNA-circ02771) reduced (P<0.05) the expression of miR-194b and led to apoptosis and inflammation. Circ02771 also enhanced the expression of transforming growth factor beta-induced factor homeobox 1 (TGIF1), which is a target gene of miR-194b. Overall, this study suggests that the circ02771/miR-194b/TGIF1 axis plays a role in mediating the effects of NH4Cl on BMECs. Therefore, this axis provides a novel target to help control hazards within the mammary gland from high circulating NH4Cl levels.http://www.sciencedirect.com/science/article/pii/S2095311921638121NH4Clcirc02771miR-194bTGIF1bovine mammary epithelial cells |
| spellingShingle | Zhi CHEN Yu-sheng LIANG Wei-cheng ZONG Jia-he GUO Jing-peng ZHOU Yong-jiang MAO De-jun JI Pei-xin JIAO LOOR Juan J Zhang-ping YANG NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis Journal of Integrative Agriculture NH4Cl circ02771 miR-194b TGIF1 bovine mammary epithelial cells |
| title | NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis |
| title_full | NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis |
| title_fullStr | NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis |
| title_full_unstemmed | NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis |
| title_short | NH4Cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771/miR-194b/TGIF1 axis |
| title_sort | nh4cl promotes apoptosis and inflammation in bovine mammary epithelial cells via the circ02771 mir 194b tgif1 axis |
| topic | NH4Cl circ02771 miR-194b TGIF1 bovine mammary epithelial cells |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921638121 |
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