Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis
The distribution of As, Cd, Cu and Se among biomolecules of different molecular weight (MW) in the heat-treated cytosolic fraction of livers and gonads of white suckers (WS; Catostomus commersonii) collected in a reference lake and in a lake subject to multi-metal contamination was investigated. Dis...
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MDPI AG
2018-09-01
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| Series: | Environments |
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| Online Access: | http://www.mdpi.com/2076-3298/5/9/102 |
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| author | Nastassia Urien Sabrina Jacob Patrice Couture Peter G. C. Campbell |
| author_facet | Nastassia Urien Sabrina Jacob Patrice Couture Peter G. C. Campbell |
| author_sort | Nastassia Urien |
| collection | DOAJ |
| description | The distribution of As, Cd, Cu and Se among biomolecules of different molecular weight (MW) in the heat-treated cytosolic fraction of livers and gonads of white suckers (WS; Catostomus commersonii) collected in a reference lake and in a lake subject to multi-metal contamination was investigated. Distribution profiles were obtained by separation of the heat-stable protein and peptide (HSP) fractions using size-exclusion high performance-liquid chromatography, coupled online to an inductively coupled plasma mass spectrometer, to quantify the associated metals. Metal-handling strategies did not vary between the reference and exposed fish, with the exception of As. Cadmium and Cu appeared associated with the heat-stable peptides metallothioneins (MTs), indicating their reasonable detoxification and regulation in WS. In contrast, Se and As were not bound to MTs, but rather, to biomolecules of lower MW (<2 kDa). Arsenic was found associated with the same biomolecules in fish from both lakes, but their proportions changed between reference and exposed fish. For future work, the identification of the Se and As binding biomolecules would be of great interest to determine if these metalloids are detoxified or if, conversely, the biomolecules are metal-sensitive and their binding to Se or As represents a threat for the health of these fish. |
| first_indexed | 2024-04-11T11:05:15Z |
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| id | doaj.art-78de457c49c6497398b3fb9a6dde6a86 |
| institution | Directory Open Access Journal |
| issn | 2076-3298 |
| language | English |
| last_indexed | 2024-04-11T11:05:15Z |
| publishDate | 2018-09-01 |
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| record_format | Article |
| series | Environments |
| spelling | doaj.art-78de457c49c6497398b3fb9a6dde6a862022-12-22T04:28:24ZengMDPI AGEnvironments2076-32982018-09-015910210.3390/environments5090102environments5090102Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS AnalysisNastassia Urien0Sabrina Jacob1Patrice Couture2Peter G. C. Campbell3Institut National de la Recherche Scientifique, Centre Eau Terre Environnement (INRS–ETE), 490 rue de la Couronne, Québec, QC G1K 9A9, CanadaInstitut National de la Recherche Scientifique, Centre Eau Terre Environnement (INRS–ETE), 490 rue de la Couronne, Québec, QC G1K 9A9, CanadaInstitut National de la Recherche Scientifique, Centre Eau Terre Environnement (INRS–ETE), 490 rue de la Couronne, Québec, QC G1K 9A9, CanadaInstitut National de la Recherche Scientifique, Centre Eau Terre Environnement (INRS–ETE), 490 rue de la Couronne, Québec, QC G1K 9A9, CanadaThe distribution of As, Cd, Cu and Se among biomolecules of different molecular weight (MW) in the heat-treated cytosolic fraction of livers and gonads of white suckers (WS; Catostomus commersonii) collected in a reference lake and in a lake subject to multi-metal contamination was investigated. Distribution profiles were obtained by separation of the heat-stable protein and peptide (HSP) fractions using size-exclusion high performance-liquid chromatography, coupled online to an inductively coupled plasma mass spectrometer, to quantify the associated metals. Metal-handling strategies did not vary between the reference and exposed fish, with the exception of As. Cadmium and Cu appeared associated with the heat-stable peptides metallothioneins (MTs), indicating their reasonable detoxification and regulation in WS. In contrast, Se and As were not bound to MTs, but rather, to biomolecules of lower MW (<2 kDa). Arsenic was found associated with the same biomolecules in fish from both lakes, but their proportions changed between reference and exposed fish. For future work, the identification of the Se and As binding biomolecules would be of great interest to determine if these metalloids are detoxified or if, conversely, the biomolecules are metal-sensitive and their binding to Se or As represents a threat for the health of these fish.http://www.mdpi.com/2076-3298/5/9/102subcellular distributionmetal traffickingmetal detoxificationmetal regulationwild fishcontamination gradientcytosolic biomoleculessize-exclusion high-performance liquid chromatography (HPLC)metallothioneinmetallomics |
| spellingShingle | Nastassia Urien Sabrina Jacob Patrice Couture Peter G. C. Campbell Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis Environments subcellular distribution metal trafficking metal detoxification metal regulation wild fish contamination gradient cytosolic biomolecules size-exclusion high-performance liquid chromatography (HPLC) metallothionein metallomics |
| title | Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis |
| title_full | Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis |
| title_fullStr | Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis |
| title_full_unstemmed | Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis |
| title_short | Cytosolic Distribution of Metals (Cd, Cu) and Metalloids (As, Se) in Livers and Gonads of Field-Collected Fish Exposed to an Environmental Contamination Gradient: An SEC-ICP-MS Analysis |
| title_sort | cytosolic distribution of metals cd cu and metalloids as se in livers and gonads of field collected fish exposed to an environmental contamination gradient an sec icp ms analysis |
| topic | subcellular distribution metal trafficking metal detoxification metal regulation wild fish contamination gradient cytosolic biomolecules size-exclusion high-performance liquid chromatography (HPLC) metallothionein metallomics |
| url | http://www.mdpi.com/2076-3298/5/9/102 |
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