| Summary: | Mycotoxin contamination in agro-food systems has been a serious concern globally during the last few decades. Mycotoxins are toxic secondary metabolites produced by fungi when they grow in agro-food products and feedstuff. Several detection techniques have been developed in recent years to detect mycotoxins in the food and feed effectively. HPLC based techniques are very common in usage in the laboratories for the testing of mycotoxins. In recent years, immuno-based assays is widely used and have been reported at large due to its sensitivity and limited detection time. Immuno assay-based kits were developed effectively to be used in the fields and in storage systems to detect the mycotoxin levels. Microarray-based immunoassays developed in the recent years could simultaneously detect aflatoxin, ochratoxin, and zearalenone with the higher sensitivity. Aptamer-based assays could target the detection of ochratoxin and aflatoxins and fumonisins at high specificity in food products. In recent years, several assays reported for the simultaneous multiple detection of different mycotoxin was based on HPLC and LC-MS/MS. There is a need for the use of these advanced technologies in the commercial scale.
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