Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control
ABSTRACTThe widespread prevalence of Helicobacter pylori infection, particularly in China, contributes to the development of gastrointestinal diseases. Antibiotics have limitations, including adverse reactions and increased antibiotic resistance. Therefore, identification of novel gastrogenic probio...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2313770 |
| _version_ | 1797319218774933504 |
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| author | Zhenhui Chen Ziyu Tang Wendan Li Xiaoshi Deng Lu Yu Jixiang Yang Jiaxin Liu Yunshui Cheng Wanwen Huang Xiaotong Guo Jiamin Shan Daixuan Zhou Weisen Zeng Yang Bai Hongying Fan |
| author_facet | Zhenhui Chen Ziyu Tang Wendan Li Xiaoshi Deng Lu Yu Jixiang Yang Jiaxin Liu Yunshui Cheng Wanwen Huang Xiaotong Guo Jiamin Shan Daixuan Zhou Weisen Zeng Yang Bai Hongying Fan |
| author_sort | Zhenhui Chen |
| collection | DOAJ |
| description | ABSTRACTThe widespread prevalence of Helicobacter pylori infection, particularly in China, contributes to the development of gastrointestinal diseases. Antibiotics have limitations, including adverse reactions and increased antibiotic resistance. Therefore, identification of novel gastrogenic probiotics capable of surviving the acidic gastric environment and effectively combating H. pylori infection has potential in restoring gastric microbiota homeostasis. Five novel strains of human gastrogenic Weizmannia coagulans (BCF-01–05) were isolated from healthy gastric mucosa and characterized using 16S rDNA identification. Acid resistance, H. pylori inhibition, and adherence to gastric epithelial cells were evaluated in in-vitro experiments and the molecular mechanism explored in in-vivo experiments. Among the gastric-derived W. coagulans strains, BCF-01 exhibited the strongest adhesion and H. pylori inhibition, warranting further in-vivo safety evaluation. Through 16S rRNA sequencing of a mouse model, BCF-01 was determined to significantly restore H. pylori-associated gastric dysbiosis and increase the abundance of potential probiotic bacteria. Furthermore, BCF-01 enhanced mucosal tight junction protein expression and inhibited the TLR4-NFκB-pyroptosis signaling pathway in macrophages, as demonstrated by qRT-PCR and western blotting.These findings highlight the potential of BCF-01 in the prevention and control of H. pylori infection. Specifically, treatment with BCF-01 effectively restored gastric microecology and improved H. pylori-mediated mucosal barrier destruction while reducing inflammation through inhibition of the TLR4-NFκB-pyroptosis signaling pathway in macrophages. BCF-01 is a promising alternative to traditional triple therapy for H. pylori infections, offering minimal side effects with high suitability for high-risk individuals. |
| first_indexed | 2024-03-08T04:02:39Z |
| format | Article |
| id | doaj.art-78e279e707bb4b46a6187edf96e4d10b |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-03-08T04:02:39Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-78e279e707bb4b46a6187edf96e4d10b2024-02-09T11:00:58ZengTaylor & Francis GroupGut Microbes1949-09761949-09842024-12-0116110.1080/19490976.2024.2313770Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection controlZhenhui Chen0Ziyu Tang1Wendan Li2Xiaoshi Deng3Lu Yu4Jixiang Yang5Jiaxin Liu6Yunshui Cheng7Wanwen Huang8Xiaotong Guo9Jiamin Shan10Daixuan Zhou11Weisen Zeng12Yang Bai13Hongying Fan14Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Inst. of Gastroenterology of Guangdong Province, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Cell Biology, School of Basic Medicine, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Inst. of Gastroenterology of Guangdong Province, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Microbiology, Guangdong Provincial Key Laboratory of Tropical, Disease Research, School of Public Health, Southern Medical University, Guangzhou, ChinaABSTRACTThe widespread prevalence of Helicobacter pylori infection, particularly in China, contributes to the development of gastrointestinal diseases. Antibiotics have limitations, including adverse reactions and increased antibiotic resistance. Therefore, identification of novel gastrogenic probiotics capable of surviving the acidic gastric environment and effectively combating H. pylori infection has potential in restoring gastric microbiota homeostasis. Five novel strains of human gastrogenic Weizmannia coagulans (BCF-01–05) were isolated from healthy gastric mucosa and characterized using 16S rDNA identification. Acid resistance, H. pylori inhibition, and adherence to gastric epithelial cells were evaluated in in-vitro experiments and the molecular mechanism explored in in-vivo experiments. Among the gastric-derived W. coagulans strains, BCF-01 exhibited the strongest adhesion and H. pylori inhibition, warranting further in-vivo safety evaluation. Through 16S rRNA sequencing of a mouse model, BCF-01 was determined to significantly restore H. pylori-associated gastric dysbiosis and increase the abundance of potential probiotic bacteria. Furthermore, BCF-01 enhanced mucosal tight junction protein expression and inhibited the TLR4-NFκB-pyroptosis signaling pathway in macrophages, as demonstrated by qRT-PCR and western blotting.These findings highlight the potential of BCF-01 in the prevention and control of H. pylori infection. Specifically, treatment with BCF-01 effectively restored gastric microecology and improved H. pylori-mediated mucosal barrier destruction while reducing inflammation through inhibition of the TLR4-NFκB-pyroptosis signaling pathway in macrophages. BCF-01 is a promising alternative to traditional triple therapy for H. pylori infections, offering minimal side effects with high suitability for high-risk individuals.https://www.tandfonline.com/doi/10.1080/19490976.2024.2313770Human gastrogenic probioticweizmannia coagulans BCF-01helicobacter pylori infectiongastric microbiota homeostasismucosal immunity |
| spellingShingle | Zhenhui Chen Ziyu Tang Wendan Li Xiaoshi Deng Lu Yu Jixiang Yang Jiaxin Liu Yunshui Cheng Wanwen Huang Xiaotong Guo Jiamin Shan Daixuan Zhou Weisen Zeng Yang Bai Hongying Fan Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control Gut Microbes Human gastrogenic probiotic weizmannia coagulans BCF-01 helicobacter pylori infection gastric microbiota homeostasis mucosal immunity |
| title | Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control |
| title_full | Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control |
| title_fullStr | Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control |
| title_full_unstemmed | Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control |
| title_short | Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control |
| title_sort | weizmannia coagulans bcf 01 a novel gastrogenic probiotic for helicobacter pylori infection control |
| topic | Human gastrogenic probiotic weizmannia coagulans BCF-01 helicobacter pylori infection gastric microbiota homeostasis mucosal immunity |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2313770 |
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