Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis)
This study was conducted to evaluate the effect of cholesterol (CHO) on ovarian development of Eriocheir sinensis through a feeding trial. Crabs (average weight 43.35 ± 0.05 g) were randomly fed with one of three diets: 0% CHO, 0.4% CHO and 1.6% CHO for 16 weeks. Results indicated that the hepatosom...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1070829/full |
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| author | Huixing Guo Mangmang Wang Xi Wang Kang Xiao Yangyang Huang Haokun Hua Wei Xiong Wenbin Liu Kenneth Prudence Abasubong Wei Qiang Cheng Chi Guangzhen Jiang |
| author_facet | Huixing Guo Mangmang Wang Xi Wang Kang Xiao Yangyang Huang Haokun Hua Wei Xiong Wenbin Liu Kenneth Prudence Abasubong Wei Qiang Cheng Chi Guangzhen Jiang |
| author_sort | Huixing Guo |
| collection | DOAJ |
| description | This study was conducted to evaluate the effect of cholesterol (CHO) on ovarian development of Eriocheir sinensis through a feeding trial. Crabs (average weight 43.35 ± 0.05 g) were randomly fed with one of three diets: 0% CHO, 0.4% CHO and 1.6% CHO for 16 weeks. Results indicated that the hepatosomatic index (HSI) and gonad index (GSI) of crabs fed with 0.4% CHO diet were significantly higher than those in the control group. (P < 0.05). Meanwhile, 0.4% CHO can significantly increase the gene expression of vtg and vgr in ovary (P < 0.05), thus promoting the accumulation of Vtg. The protein expression of MEK1/2, ERK, p-ERK1/2, Cyclin B and CDC2 were significantly increased in the 0.4% CHO group, therefore dietary CHO might promote oocyte maturation by activating MAPK signal pathway and cooperating with maturation promoting factor (MPF). Further exploration results showed that 0.4% CHO was able to significantly up-regulate the protein expression of STAR and SR-B1 to promote the transport of CHO to ovary, thereby providing sufficient substrates for estradiol (E2) synthesis. In addition, the results in vivo and in vitro shown that CHO could regulate the activities of enzymes such as CYP11A1 and CYP19A1 through the cAMP-PKA-CREB/SF-1 signal pathway and then affects the level of E2 in the organism. In summary, supplementing the appropriate amount of CHO in the diet can improve the E2 level of the organism, thus increasing the accumulation of nutrients in the ovary, promoting the completion of meiosis, and finally achieving the purpose of promoting ovarian maturation. |
| first_indexed | 2024-04-13T06:10:49Z |
| format | Article |
| id | doaj.art-78e2fa1bb1524985bff244df4e7360cf |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-13T06:10:49Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-78e2fa1bb1524985bff244df4e7360cf2022-12-22T02:59:03ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-12-01910.3389/fmars.2022.10708291070829Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis)Huixing GuoMangmang WangXi WangKang XiaoYangyang HuangHaokun HuaWei XiongWenbin LiuKenneth Prudence AbasubongWei QiangCheng ChiGuangzhen JiangThis study was conducted to evaluate the effect of cholesterol (CHO) on ovarian development of Eriocheir sinensis through a feeding trial. Crabs (average weight 43.35 ± 0.05 g) were randomly fed with one of three diets: 0% CHO, 0.4% CHO and 1.6% CHO for 16 weeks. Results indicated that the hepatosomatic index (HSI) and gonad index (GSI) of crabs fed with 0.4% CHO diet were significantly higher than those in the control group. (P < 0.05). Meanwhile, 0.4% CHO can significantly increase the gene expression of vtg and vgr in ovary (P < 0.05), thus promoting the accumulation of Vtg. The protein expression of MEK1/2, ERK, p-ERK1/2, Cyclin B and CDC2 were significantly increased in the 0.4% CHO group, therefore dietary CHO might promote oocyte maturation by activating MAPK signal pathway and cooperating with maturation promoting factor (MPF). Further exploration results showed that 0.4% CHO was able to significantly up-regulate the protein expression of STAR and SR-B1 to promote the transport of CHO to ovary, thereby providing sufficient substrates for estradiol (E2) synthesis. In addition, the results in vivo and in vitro shown that CHO could regulate the activities of enzymes such as CYP11A1 and CYP19A1 through the cAMP-PKA-CREB/SF-1 signal pathway and then affects the level of E2 in the organism. In summary, supplementing the appropriate amount of CHO in the diet can improve the E2 level of the organism, thus increasing the accumulation of nutrients in the ovary, promoting the completion of meiosis, and finally achieving the purpose of promoting ovarian maturation.https://www.frontiersin.org/articles/10.3389/fmars.2022.1070829/fullEriocheir sinensischolesterolovarian developmentestradiol synthesisvitellogenesis |
| spellingShingle | Huixing Guo Mangmang Wang Xi Wang Kang Xiao Yangyang Huang Haokun Hua Wei Xiong Wenbin Liu Kenneth Prudence Abasubong Wei Qiang Cheng Chi Guangzhen Jiang Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis) Frontiers in Marine Science Eriocheir sinensis cholesterol ovarian development estradiol synthesis vitellogenesis |
| title | Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis) |
| title_full | Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis) |
| title_fullStr | Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis) |
| title_full_unstemmed | Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis) |
| title_short | Effect of dietary cholesterol on ovarian development of Chinese mitten crabs (Eriocheir sinensis) |
| title_sort | effect of dietary cholesterol on ovarian development of chinese mitten crabs eriocheir sinensis |
| topic | Eriocheir sinensis cholesterol ovarian development estradiol synthesis vitellogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1070829/full |
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