The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K
Subgroup K avian leukosis virus (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first defined in 2012 and has been become prevalent in Chinese native chickens in recent years. An in-depth analysis of the genetic diversity of ALV-K was performed in the study. By Blast analysis, the e...
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| Format: | Article |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1228109/full |
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| author | Jinhan Guo Qiaomu Deng Qiaomu Deng Weiyu Zhu Fumei Fu Linmin Liu Tianchao Wei Ping Wei |
| author_facet | Jinhan Guo Qiaomu Deng Qiaomu Deng Weiyu Zhu Fumei Fu Linmin Liu Tianchao Wei Ping Wei |
| author_sort | Jinhan Guo |
| collection | DOAJ |
| description | Subgroup K avian leukosis virus (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first defined in 2012 and has been become prevalent in Chinese native chickens in recent years. An in-depth analysis of the genetic diversity of ALV-K was performed in the study. By Blast analysis, the env gene and the sequences of the 25 ALV-K isolates we isolated were found to be closely related to the isolates from Guangdong, Hebei, Jiangsu, and Hubei provinces, China. Further eighty-nine sequences of the gp85 gene of ALV-K strains available were used in the phylogenetic and genetic distance analyses for the classification. ALV-K was divided into two second-order clades (Clades 1.1 and 1.2) and three third-order clades (Clades 1.2.1, 1.2.2, and 1.2.3), indicating that not only 1.1 and 1.2.3, the two old clades which are prevalent in Japan, but also two new clades (1.2.1, 1.2.2), are co-prevalent in China. The representative strains of each clade were defined for the first time. Notably, Clade 1.2.2 was found to have a deletion of an amino acid residue in the gp85 gene, which was obviously different from Clades 1.1, 1.2.1, and 1.2.3. The proposed classification method will facilitate future studies of ALV-K epidemiology and the comparison of sequences obtained across the world. The first global comprehensive molecular epidemiological analysis was accomplished on the emerging ALV-K. |
| first_indexed | 2024-03-12T21:22:47Z |
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| id | doaj.art-78e35f437b9640e49f28f2a607a41bb4 |
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| language | English |
| last_indexed | 2024-03-12T21:22:47Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-78e35f437b9640e49f28f2a607a41bb42023-07-28T13:53:25ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-07-011010.3389/fvets.2023.12281091228109The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-KJinhan Guo0Qiaomu Deng1Qiaomu Deng2Weiyu Zhu3Fumei Fu4Linmin Liu5Tianchao Wei6Ping Wei7Institute for Poultry Science and Health, Guangxi University, Nanning, ChinaInstitute for Poultry Science and Health, Guangxi University, Nanning, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaInstitute for Poultry Science and Health, Guangxi University, Nanning, ChinaInstitute for Poultry Science and Health, Guangxi University, Nanning, ChinaInstitute for Poultry Science and Health, Guangxi University, Nanning, ChinaInstitute for Poultry Science and Health, Guangxi University, Nanning, ChinaInstitute for Poultry Science and Health, Guangxi University, Nanning, ChinaSubgroup K avian leukosis virus (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first defined in 2012 and has been become prevalent in Chinese native chickens in recent years. An in-depth analysis of the genetic diversity of ALV-K was performed in the study. By Blast analysis, the env gene and the sequences of the 25 ALV-K isolates we isolated were found to be closely related to the isolates from Guangdong, Hebei, Jiangsu, and Hubei provinces, China. Further eighty-nine sequences of the gp85 gene of ALV-K strains available were used in the phylogenetic and genetic distance analyses for the classification. ALV-K was divided into two second-order clades (Clades 1.1 and 1.2) and three third-order clades (Clades 1.2.1, 1.2.2, and 1.2.3), indicating that not only 1.1 and 1.2.3, the two old clades which are prevalent in Japan, but also two new clades (1.2.1, 1.2.2), are co-prevalent in China. The representative strains of each clade were defined for the first time. Notably, Clade 1.2.2 was found to have a deletion of an amino acid residue in the gp85 gene, which was obviously different from Clades 1.1, 1.2.1, and 1.2.3. The proposed classification method will facilitate future studies of ALV-K epidemiology and the comparison of sequences obtained across the world. The first global comprehensive molecular epidemiological analysis was accomplished on the emerging ALV-K.https://www.frontiersin.org/articles/10.3389/fvets.2023.1228109/fullsubgroup K avian leukosis virusmolecular epidemiologyphylogenetic analysisclassificationgenetic distanceclades |
| spellingShingle | Jinhan Guo Qiaomu Deng Qiaomu Deng Weiyu Zhu Fumei Fu Linmin Liu Tianchao Wei Ping Wei The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K Frontiers in Veterinary Science subgroup K avian leukosis virus molecular epidemiology phylogenetic analysis classification genetic distance clades |
| title | The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K |
| title_full | The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K |
| title_fullStr | The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K |
| title_full_unstemmed | The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K |
| title_short | The phylogenetic analysis of the new emerging ALV-K revealing the co-prevailing of multiple clades in chickens and a proposal for the classification of ALV-K |
| title_sort | phylogenetic analysis of the new emerging alv k revealing the co prevailing of multiple clades in chickens and a proposal for the classification of alv k |
| topic | subgroup K avian leukosis virus molecular epidemiology phylogenetic analysis classification genetic distance clades |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1228109/full |
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