Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus
Methicillin-resistant Staphylococcus aureus is a problem in both healthcare institutions and community settings. This is due to its multi-drug resistant challenges. Hence, this study assessed the prevalence of methicillin resistant gene (mecA), exfoliative toxin (eta and etb) and toxic shock syndrom...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-03-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853517301889 |
| _version_ | 1818957102409842688 |
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| author | Cajethan Ezeamagu Irene Imanatue Margaret Dosunmu Adebola Odeseye Glory Baysah Daniel Aina Foluke Odutayo Grace Mensah-Agyei |
| author_facet | Cajethan Ezeamagu Irene Imanatue Margaret Dosunmu Adebola Odeseye Glory Baysah Daniel Aina Foluke Odutayo Grace Mensah-Agyei |
| author_sort | Cajethan Ezeamagu |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus is a problem in both healthcare institutions and community settings. This is due to its multi-drug resistant challenges. Hence, this study assessed the prevalence of methicillin resistant gene (mecA), exfoliative toxin (eta and etb) and toxic shock syndrome (tsst-1) genes in S. aureus isolated from clinical samples. A total of 120 clinical samples of patients (urine, high vagina swab (HVS), semen, wound swab, sputum and urethral swab) from a hospital laboratory were obtained. S. aureus was isolated and then identified with API-staph kit. Antibiotic susceptibility of the isolates was determined by agar diffusion while PCR was used to detect the presence of mecA and toxin-associated genes. Fifty S. aureus isolates were obtained at frequencies of 26(52%), 12(24%), 4(8%), 3(6%), 3(6%) and 2(4%) from the HVS, urine, semen, wound, sputum and urethral swab samples respectively. All the isolates of S. aureus were resistant to the antibiotics used in this study. MecA, tsst-1, eta and etb were detected in 19(38%), 7(14%), 3(6%) and 2(4%) of the isolates respectively. The prevalence of MRSA and its resistance pattern observed in this study was a signal that the health-care workers and the general public are at risk. |
| first_indexed | 2024-12-20T11:04:31Z |
| format | Article |
| id | doaj.art-78e4a91f550944c68919738f34f2e63b |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-20T11:04:31Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-78e4a91f550944c68919738f34f2e63b2022-12-21T19:42:55ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352018-03-0171929710.1016/j.bjbas.2017.07.010Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureusCajethan Ezeamagu0Irene Imanatue1Margaret Dosunmu2Adebola Odeseye3Glory Baysah4Daniel Aina5Foluke Odutayo6Grace Mensah-Agyei7Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, NigeriaDepartment of Microbiology, Babcock University, Ilishan-Remo, Ogun State, NigeriaDepartment of Microbiology, Babcock University, Ilishan-Remo, Ogun State, NigeriaNigerian Institute of Science Laboratory Technology Ibadan, Oyo State, NigeriaDepartment of Microbiology, Babcock University, Ilishan-Remo, Ogun State, NigeriaDepartment of Microbiology, Babcock University, Ilishan-Remo, Ogun State, NigeriaDepartment of Basic Sciences, Babcock University, Ilishan-Remo, Ogun State, NigeriaDepartment of Microbiology, Babcock University, Ilishan-Remo, Ogun State, NigeriaMethicillin-resistant Staphylococcus aureus is a problem in both healthcare institutions and community settings. This is due to its multi-drug resistant challenges. Hence, this study assessed the prevalence of methicillin resistant gene (mecA), exfoliative toxin (eta and etb) and toxic shock syndrome (tsst-1) genes in S. aureus isolated from clinical samples. A total of 120 clinical samples of patients (urine, high vagina swab (HVS), semen, wound swab, sputum and urethral swab) from a hospital laboratory were obtained. S. aureus was isolated and then identified with API-staph kit. Antibiotic susceptibility of the isolates was determined by agar diffusion while PCR was used to detect the presence of mecA and toxin-associated genes. Fifty S. aureus isolates were obtained at frequencies of 26(52%), 12(24%), 4(8%), 3(6%), 3(6%) and 2(4%) from the HVS, urine, semen, wound, sputum and urethral swab samples respectively. All the isolates of S. aureus were resistant to the antibiotics used in this study. MecA, tsst-1, eta and etb were detected in 19(38%), 7(14%), 3(6%) and 2(4%) of the isolates respectively. The prevalence of MRSA and its resistance pattern observed in this study was a signal that the health-care workers and the general public are at risk.http://www.sciencedirect.com/science/article/pii/S2314853517301889S. aureusMethicillin resistanceStaphylococcal toxins |
| spellingShingle | Cajethan Ezeamagu Irene Imanatue Margaret Dosunmu Adebola Odeseye Glory Baysah Daniel Aina Foluke Odutayo Grace Mensah-Agyei Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus Beni-Suef University Journal of Basic and Applied Sciences S. aureus Methicillin resistance Staphylococcal toxins |
| title | Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus |
| title_full | Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus |
| title_fullStr | Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus |
| title_full_unstemmed | Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus |
| title_short | Detection of methicillin resistant and toxin-associated genes in Staphylococcus aureus |
| title_sort | detection of methicillin resistant and toxin associated genes in staphylococcus aureus |
| topic | S. aureus Methicillin resistance Staphylococcal toxins |
| url | http://www.sciencedirect.com/science/article/pii/S2314853517301889 |
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