Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins
The ability to fertilise an egg is acquired by the mammalian sperm during the complex biochemical process called capacitation. Capacitation is accompanied by the production of reactive oxygen species (ROS), but the mechanism of redox regulation during capacitation has not been elucidated. This study...
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/15/7903 |
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| author | Agnieszka Mostek Anna Janta Anna Majewska Andrzej Ciereszko |
| author_facet | Agnieszka Mostek Anna Janta Anna Majewska Andrzej Ciereszko |
| author_sort | Agnieszka Mostek |
| collection | DOAJ |
| description | The ability to fertilise an egg is acquired by the mammalian sperm during the complex biochemical process called capacitation. Capacitation is accompanied by the production of reactive oxygen species (ROS), but the mechanism of redox regulation during capacitation has not been elucidated. This study aimed to verify whether capacitation coincides with reversible oxidative post-translational modifications of proteins (oxPTMs). Flow cytometry, fluorescence microscopy and Western blot analyses were used to verify the sperm capacitation process. A fluorescent gel-based redox proteomic approach allowed us to observe changes in the level of reversible oxPTMs manifested by the reduction or oxidation of susceptible cysteines in sperm proteins. Sperm capacitation was accompanied with redox modifications of 48 protein spots corresponding to 22 proteins involved in the production of ROS (SOD, DLD), playing a role in downstream redox signal transfer (GAPDHS and GST) related to the cAMP/PKA pathway (ROPN1L, SPA17), acrosome exocytosis (ACRB, sperm acrosome associated protein 9, IZUMO4), actin polymerisation (CAPZB) and hyperactivation (TUBB4B, TUB1A). The results demonstrated that sperm capacitation is accompanied by altered levels of oxPTMs of a group of redox responsive proteins, filling gaps in our knowledge concerning sperm capacitation. |
| first_indexed | 2024-03-10T09:15:01Z |
| format | Article |
| id | doaj.art-78e4ff9c365441c6b4d9aa8403d58e70 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:15:01Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-78e4ff9c365441c6b4d9aa8403d58e702023-11-22T05:40:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012215790310.3390/ijms22157903Bull Sperm Capacitation Is Accompanied by Redox Modifications of ProteinsAgnieszka Mostek0Anna Janta1Anna Majewska2Andrzej Ciereszko3Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-748 Olsztyn, PolandThe ability to fertilise an egg is acquired by the mammalian sperm during the complex biochemical process called capacitation. Capacitation is accompanied by the production of reactive oxygen species (ROS), but the mechanism of redox regulation during capacitation has not been elucidated. This study aimed to verify whether capacitation coincides with reversible oxidative post-translational modifications of proteins (oxPTMs). Flow cytometry, fluorescence microscopy and Western blot analyses were used to verify the sperm capacitation process. A fluorescent gel-based redox proteomic approach allowed us to observe changes in the level of reversible oxPTMs manifested by the reduction or oxidation of susceptible cysteines in sperm proteins. Sperm capacitation was accompanied with redox modifications of 48 protein spots corresponding to 22 proteins involved in the production of ROS (SOD, DLD), playing a role in downstream redox signal transfer (GAPDHS and GST) related to the cAMP/PKA pathway (ROPN1L, SPA17), acrosome exocytosis (ACRB, sperm acrosome associated protein 9, IZUMO4), actin polymerisation (CAPZB) and hyperactivation (TUBB4B, TUB1A). The results demonstrated that sperm capacitation is accompanied by altered levels of oxPTMs of a group of redox responsive proteins, filling gaps in our knowledge concerning sperm capacitation.https://www.mdpi.com/1422-0067/22/15/7903sperm capacitation<i>Bos taurus</i>redox proteomicsreversible oxPTMs |
| spellingShingle | Agnieszka Mostek Anna Janta Anna Majewska Andrzej Ciereszko Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins International Journal of Molecular Sciences sperm capacitation <i>Bos taurus</i> redox proteomics reversible oxPTMs |
| title | Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins |
| title_full | Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins |
| title_fullStr | Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins |
| title_full_unstemmed | Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins |
| title_short | Bull Sperm Capacitation Is Accompanied by Redox Modifications of Proteins |
| title_sort | bull sperm capacitation is accompanied by redox modifications of proteins |
| topic | sperm capacitation <i>Bos taurus</i> redox proteomics reversible oxPTMs |
| url | https://www.mdpi.com/1422-0067/22/15/7903 |
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