Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica
The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/11/1495 |
| _version_ | 1818501218161393664 |
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| author | Jiří Pazourek Karel Šmejkal |
| author_facet | Jiří Pazourek Karel Šmejkal |
| author_sort | Jiří Pazourek |
| collection | DOAJ |
| description | The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-d-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed. |
| first_indexed | 2024-12-10T20:53:10Z |
| format | Article |
| id | doaj.art-78e60597cdcc4d3f98d740c413afaaed |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T20:53:10Z |
| publishDate | 2016-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-78e60597cdcc4d3f98d740c413afaaed2022-12-22T01:34:03ZengMDPI AGMolecules1420-30492016-11-012111149510.3390/molecules21111495molecules21111495Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnificaJiří Pazourek0Karel Šmejkal1Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tr. 1946/1, Brno 61242, Czech RepublicDepartment of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého tr. 1946/1, Brno 61242, Czech RepublicThe use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-d-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.http://www.mdpi.com/1420-3049/21/11/1495fraction collectionevaporative light scattering detectorhydrophilic interaction liquid chromatographymixed-mode chromatographyPectinatella magnifica |
| spellingShingle | Jiří Pazourek Karel Šmejkal Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica Molecules fraction collection evaporative light scattering detector hydrophilic interaction liquid chromatography mixed-mode chromatography Pectinatella magnifica |
| title | Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica |
| title_full | Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica |
| title_fullStr | Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica |
| title_full_unstemmed | Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica |
| title_short | Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica |
| title_sort | feasibility of fraction collection in hplc systems with evaporative light scattering detector analysis of pectinatella magnifica |
| topic | fraction collection evaporative light scattering detector hydrophilic interaction liquid chromatography mixed-mode chromatography Pectinatella magnifica |
| url | http://www.mdpi.com/1420-3049/21/11/1495 |
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